欢迎访问作物学报,今天是

作物学报 ›› 2014, Vol. 40 ›› Issue (11): 2046-2051.doi: 10.3724/SP.J.1006.2014.02046

• 研究简报 • 上一篇    下一篇

不同水氮处理对豫麦49-198籽粒抗氧化物含量的影响

孙德祥,马冬云*,王晨阳,李耀光,刘卫星,李秋霞,冯伟,郭天财*   

  1. 河南农业大学/国家小麦工程技术研究中心, 河南郑州450002
  • 收稿日期:2014-02-21 修回日期:2014-09-16 出版日期:2014-11-12 网络出版日期:2014-09-26
  • 通讯作者: 马冬云, E-mail: xmzxmdy@126.com; 郭天财, E-mail: tcguo888@sina.com
  • 基金资助:

    本研究由国家“十二五”科技支撑计划项目(2012BAD04B07), 国家现代农业产业体系建设专项(CARS-03), 国家公益性行业(农业)专项(201203031)资助。

Effects of Irrigation and Nitrogen on Antioxidant Contents in Yumai 49-198 Grains

SUN De-Xiang,MA Dong-Yun*,WANG Chen-Yang,LI Yao-Guang,LIU Wei-Xing,LI Qiu-Xia,FENG Wei,GUO Tian-Cai*   

  1. National Engineering Research Center for Wheat / Henan Agricultural University, Zhengzhou 450002, China
  • Received:2014-02-21 Revised:2014-09-16 Published:2014-11-12 Published online:2014-09-26

摘要:

2012-2013年度, 在温县和郑州大田条件下, 研究不同水氮处理对冬小麦品种豫麦49-198籽粒总酚、类黄酮、类胡萝卜素含量及抗氧化活性的影响。结果表明, 在施纯氮0~300 kg hm–2范围内, 所有观测指标均随施氮量的增加而增加, 以施氮300 kg hm–2处理最高。随灌水次数(0~2)的增加, 总酚、类黄酮含量和抗氧化活性呈先增加后降低趋势, 以灌拔节水处理最高;类胡萝卜素含量在不同试点间表现不一致。水氮耦合, 以灌拔节水+施氮240~300 kg hm–2处理的抗氧化物含量及抗氧化活性较高, 而总酚、类黄酮及类胡萝卜素的积累量则以灌拔节和开花水+施氮240~300 kg hm–2处理较高。相关分析表明, 籽粒总酚、类黄酮含量与抗氧化活性均呈显著正相关, 表明总酚、类黄酮含量增加可以提高小麦籽粒抗氧化活性;不同深度土层土壤水分含量及硝态氮含量与籽粒抗氧化物质含量的相关性存在差异, 总体而言, 氮含量有助于总酚及类胡萝卜素含量的积累, 而水分含量可能有助于类黄酮含量的提高。

关键词: 灌水, 氮肥, 冬小麦, 抗氧化物质, 籽粒

Abstract:

Two field experiments were carried out in Wenxian and Zhengzhou of Henan Province in 2012–2013 wheat season to study the effects of nitrogen (N) application rate and irrigation on total phenol, flavonoids, and carotenoid contents and antioxidant activity in winter wheat cultivar Yumai 49-198. In the N application range of 0–300 kg ha-1, all indices tested increased with increasing N rate and reached peak values under 300 kg ha-1. With the increase of irrigation frequency (0–2 irrigations), total phenol and flavonoids contents and antioxidant activity had the maximum values under one irrigation condition at jointing, whereas the carotenoid content varied across locations. The interaction between N application rate and irrigation had a significant effect on antioxidants in wheat grain. Treatments of irrigation at jointing stage plus N application of 240300 kg ha–1 resulted in the high antioxidant content and antioxidant activity, whereas twice irrigation at jointing and anthesis stages plus N application of 240300 kg ha–1 resulted in antioxidant accumulation. Total phenol and flavonoids contents were positively correlated with antioxidant activity, indicating that high contents of total phenol and flavonoids were favorable for high antioxidation. The correlations of grain antioxidant content with soil moisture and NO3--N content varied in different soil layers. Generally, N fertilizer had the effect on increasing total phenol and carotenoid contents and soil water had the effect on accumulating flavonoids.

Key words: Irrigation, Nitrogen fertilization, Winter wheat, Antioxidant, Grain

[1]石碧, 狄莹. 植物多酚. 北京: 科学出版社, 2000



Shi B, Di Y. Plant Polyphenol. Beijing: Science Press, 2000 (in Chinese)



[2]Onyeneho S N, Hettiarachchi N S. Antioxidant activity of durum wheat bran. J Agric Food Sci, 1992, 40: 1496–1500



[3]Liu S, Stampfer H J, Giovannucci E, Rimm E, Manson J E, Hennekens C H, Willett W C. Whole grain consumption and risk of coronary heart disease: Result from the nurses’  health study. Am Clin Nutr, 2000, 71: 412–419



[4]Liu S, Stampfer H J, Rexrode, K M, Hu F B, Willett W C. Whole grain consumption and risk of ischemic stroke in women: a prospective study. Am Med Assoc, 2000, 90: 1534–1540



[5]Liu S, Stampfer H J, Rexrode, K M, Hu F B, Giovannucci E, Colditz G A, Manson Je, Hennekens C H, Willett W C. A prospective study of whole grain intake and risk of type 2 diabetes mellitus in US women. Am Public Health, 2000, 90: 1409–1415



[6]Marquart L, Jacobs D R, Slavin J L. Whole grains and health: An overview. Am Coll Nutr, 2000, 19: 289S–301S



[7]Fester T, Schmidt D, Lohse S, Walter M H, Giuliano G, Bramley P M, Fraser P D, Hause B, Strack D. Stimulation of carotenoid metabolism in arbuscular mycorrhizal roots. Planta, 2002, 216: 148–154



[8]宗学凤, 张建奎, 李帮秀, 余国东, 石有明, 王三根. 小麦籽粒颜色与抗氧化作用. 作物学报, 2006, 62: 238–241



Zong X F, Zhang J K, Li B X, Yu G D, Shi Y M, Wang S G. Relationship between antioxidation and grain colors of wheat (Ttiticum aestivum L.). Acta Agron Sin, 2006, 32: 238–241 (in Chinese with English abstract)



[9]Engert N, John A, Henning W, Honermeier B. Effect of sprouting on the concentration of phenolic acids and antioxidative capacity in wheat cultivars (Triticum aestivum ssp. aestivum L.) in dependency of nitrogen fertilization. J Appl Bot Food Q, 2011, 84: 111–118



[10]Alizdeh A, Khoshkhui M, Javidnia K, Firuzi O, Tafazoli E, Khalighi. Effect of fertilizer on yield, essential oil composition, total phenolic content and antioxidant activity in Satureja hortensis L. (Lamiaceae) cultivated in Iran. J Med Plant Res, 2010, 4: 33–39



[11]Konopka I, Tanska M, Faron A, Stepien A, Wojtkowiak K. Comparison of the phenolic compounds, carotenoids and tocochromanols content in wheat grain under organic and mineral fertilization regimes. Molecules, 2012, 17: 12341–12355



[12]梁宗锁, 杨东风, 杨宗岐, 韩蕊莲, 刘晓蕾. 不同土壤水分对丹参叶片总酚酸类成分积累及相关酶活性的影响. 浙江理工大学学报, 2013, 30: 573–578



Liang Z S, Yang D F, Yang Z Q, Han R L, Liu X L. Effect of different soil moistures on total phenolic acid composition accumulation and relevant enzymatic activity of Salvia miltiorrhiza Lamina. J Zhejiang Sci-Tech Univ, 2013, 30: 573–578 (in Chinese with English abstract)



[13]王爱华, 王松峰, 宫长荣. 氮素用量对烤烟上部叶片多酚类物质动态的影响. 西北农林科技大学学报(自然科学版), 2005, 33(3): 57–60



Wang A H, Wang S F, Gong C R. The dynamic effects of nitrogen rate on polyphenols of flue-cured tobacco upper leaf. J Northwest Sci Tech Univ Agric For (Nat Sci Edn), 2005, 33(3): 57–60 (in Chinese with English abstract)



[14]American Association of Cereal Chemist: Approved Methods of the AACC, 9th Edn. The Association: St. Paul, MN, U.S.A. 1995



[15]Shen Y, Jin L, Xiao P, Lu Y, Bao J S. Total phenolics, ?avonoids, antioxidant capacity in rice grain and their relations to grain color, size and weight. J Cereal Sci, 2009, 49: 106–111



[16]Bao J S, Cai Y, Sun M, Wang G Y, Corke H. Anthocyanins, ?avonols, and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability. J Agric Food Chem, 2005, 53: 2327–2332



[17]Miller N J, Diplock A T, Rice E C, Davies M J, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci, 1993, 84: 407–412



[18]Mogren L M, Olsson M E, Gertsson U E. Quercetin content in field-cured onions (Allium cepa L.): effects of cultivar, lifting time, and nitrogen fertilizer level. J Agric Food Chem, 2006, 54: 6185–6191



[19]Nguyen P M, Niemeyer E D. Effects of nitrogen fertilization on the phenolic composition and antioxidant properties of Basil (Ocimum basilicum L.). J Agric Food Chem, 2008, 56: 8685–8691



[20]王祥军, 齐军仓, 贾力群, 王倩, 王琴, 马建峰, 王仙, 曹连莆. 氮素对大麦籽粒中酚酸及蛋白质含量的影响. 核农学报, 2011, 25: 162–168



Wang X J, Qi J C, Jia L Q, Wang Q, Wang Q, Ma J F, Wang X, Cao L P. Effect of nitrogen on phenolic acid and protein content in barley (Hordeum vulgare L.) grain. J Nucl Agric Sci, 2011, 25: 162–168 (in Chinese with English abstract)



[21]Margna U. Control at the level of substrate supply: an alternative in the regulation of phenylpropanoid accumulation in plant cells. Phytochemistry, 1977, 16: 419–426



[22]Shih C H, Chu H, Tang L K, Sakamoto W, Maekawa M, Chu I K, Wang M F, Lo C. Functional characterization of key structural genes in rice flavonoid biosynthesis. Planta, 2008, 228: 1043–1054



[23]Herms D A, Mattson W J. The dilemma of plants: to grow or defend. Q Rev Biol, 1992, 67: 283–335



[24]Bryant J P, Chapin III F S, Klein D R. Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory. J Ecol, 1983, 40: 357–368



[25]冯虎元, 安黎哲, 陈书燕, 王勋陵, 程国栋. 增强UV-B辐射与干旱复合处理对小麦幼苗生理特性的影响. 生态学报, 2002, 22: 1564–1567



Feng H Y, An L Z, Chen S Y, Wang X L, Cheng G D. The interactive effect of enhanced UV-B irradiation and water deficit on physiological properties of spring wheat seeding. Acta Ecol Sin, 2002, 22: 1564–1567 (in Chinese with English abstract)



[26]陈义强, 刘国顺, 凌爱芬, 张毅, 王芳, 习红昂. 氮、磷、钾肥和土壤水分对烟草烤后叶中类胡萝卜素含量的影响. 植物生理学通讯, 2008, 44: 279–282



Chen Y Q, Liu G S, Ling A F, Zhang Y, Wang F, Xi H A. Effects of nitrogen, phosphate, potassium fertilizers and soil water on cartenoids contents of flue-cured tobacco (Nicotiana tabacum L.) leaves. Plant Physiol Commun, 2008, 44: 279–282 (in Chinese with English abstract)



[27]Oliveira C, Silva Ferreira A C, Mendes Pinto M, Hogg T, Alves F, Guedes D E, Pinho P. Carotenoid compounds in grapes and their relationship to plant water status. J Agric Food Chem, 2003, 51: 5967–5971



[28]He X Y, He Z H, Ma W, Appels R, Xia X C. Allelic variants of phytoene synthase1 (Psy1) genes in Chinese and CIMMYT wheat cultivars and development of functional markers for ?our colour. Mol Breed, 2009, 23: 553–563

[1] 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[2] 张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535.
[3] 王壮壮, 武紫君, 张永新, 张芯源, 袁丽雪, 陈如雪, 刘世举, 段剑钊, 冯伟, 王同朝, 王永华. 豫东南黏壤潮土区水氮优化协同提高冬小麦产量和氮素利用效率[J]. 作物学报, 2026, 52(5): 1501-1521.
[4] 乔宇馨, 李程越, 康晓玉, 张鑫琪, 贾绍辉, 刘倩, 曹亚丽, 史鑫蕊, 郝兴宇, 李萍. 基于APSIM模型的长期免耕秸秆覆盖对旱地小麦增产效应研究[J]. 作物学报, 2026, 52(4): 1181-1192.
[5] 侯思宇, 王国璀, 韦金贵, 谢玮欣, 殷文, 樊志龙, 柴强, 胡发龙. 绿肥配施化学氮肥对西北干旱灌区小麦干物质积累及产量形成的影响[J]. 作物学报, 2026, 52(4): 1208-1219.
[6] 于天一, 王春晓, 肖丽, 钟召迪, 王宣仓, 赵勇, 路亚, 吴月, 吴正锋. 不同结瘤特性花生品种氮素累积、产量及品质特性对氮肥用量的响应[J]. 作物学报, 2026, 52(3): 881-894.
[7] 李晓龙, 严清彪, 李正鹏, 殷文, 樊志龙, 胡发龙, 韩梅, 柴强. 氮肥减施协同绿肥过腹还田可降低青海高原小麦农田温室气体排放[J]. 作物学报, 2026, 52(3): 908-921.
[8] 谢玮欣, 毛守发, 韦金贵, 侯思宇, 樊志龙, 殷文, 胡发龙, 南运有, 柴强. 干旱灌区绿肥还田对减量灌水春小麦水分利用的调控效应[J]. 作物学报, 2026, 52(2): 514-526.
[9] 许倍铭, 郝紫瑞, 冯健超, 马耕, 王丽芳, 谢迎新, 王晨阳, 马冬云. 氮磷减施对不同强筋小麦品种产量和品质及其土壤生物学特性的影响[J]. 作物学报, 2026, 52(2): 603-619.
[10] 石吕, 石晓旭, 韩笑, 单海勇, 刘旭杰, 张晋, 严旖旎, 李赢, 刘海翠, 魏亚凤, 杨美英, 薛亚光, 刘建, 张祖建. 氮肥减量与追施方式对小麦产量和氮肥利用效率及麦田N2O排放的影响[J]. 作物学报, 2026, 52(1): 202-220.
[11] 朱家宝, 王先领, 樊友众, 王宗铠, 蒯婕, 汪波, 王晶, 徐正华, 赵杰, 周广生. 秸秆还田耦合氮肥运筹对稻茬油菜茎秆质量和抗倒伏性能的影响[J]. 作物学报, 2026, 52(1): 233-248.
[12] 付江鹏, 柳发财, 闫宝琴, 王永栋, 李利利, 魏玮, 周英霞. 控释肥替代普通尿素对旱作高粱干物质积累分配、产量和品质的影响[J]. 作物学报, 2025, 51(9): 2501-2513.
[13] 杨颖聪, 张俊豪, 唐一哲, 乔唱唱, 王鹏博, 黄明, 徐国伟, 王贺正. 秸秆还田和施磷量对旱地小麦籽粒淀粉及其合成相关酶活性的影响[J]. 作物学报, 2025, 51(9): 2467-2484.
[14] 樊友众, 王先领, 王宗铠, 王春云, 王天尧, 谢捷, 蒯婕, 汪波, 王晶, 徐正华, 赵杰, 周广生. 秸秆还田耦合氮肥运筹对稻茬油菜光合性能及产量的影响[J]. 作物学报, 2025, 51(8): 2139-2151.
[15] 杨婷婷, 陈娟, ABDUL Rehman, 李婧, 闫素辉, 汪建来, 李文阳. 花后弱光对软质小麦干物质积累转运、籽粒产量和淀粉品质的影响[J]. 作物学报, 2025, 51(8): 2204-2219.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!