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作物学报 ›› 2015, Vol. 41 ›› Issue (02): 197-206.doi: 10.3724/SP.J.1006.2015.00197

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

河南省近年审定小麦品种基于系谱和SNP标记的遗传多样性分析

曹廷杰1,2,谢菁忠1,吴秋红1,陈永兴1,王振忠1,赵虹2,王西成2,詹克慧3,徐如强4,王际睿5,罗明成6,刘志勇1,*   

  1. 1中国农业大学植物遗传育种系, 北京 100193; 2河南省农业科学院小麦研究所, 河南郑州 450002; 3河南农业大学, 河南郑州 450002; 4郑州大学生命科学院, 河南郑州 450001; 5四川农业大学小麦研究所, 四川成都 611130; 6 University of California at Davis, Davis, CA 95616, USA
  • 收稿日期:2014-06-30 修回日期:2014-12-19 出版日期:2015-02-12 网络出版日期:2014-12-29
  • 通讯作者: 刘志勇, E-mail: zhiyongliu@cau.edu.cn
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2011CB100104)资助。

Genetic Diversity of Registered Wheat Varieties in Henan Province Based on Pedigree and Single-Nucleotide Polymorphism

CAO Ting-Jie1,2,XIE Jing-Zhong1,WU Qiu-Hong1,CHEN Yong-Xing1,WANG Zhen-Zhong1,ZHAO Hong2,WANG Xi-Cheng2,ZHAN Ke-Hui3,XU Ru-Qiang4,WANG Ji-Rui5,LUO Ming-Cheng6,LIU Zhi-Yong1,*   

  1. 1 Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China; 2 Wheat Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3 Henan Agricultural University, Zhengzhou 450002, China; 4 School of Life Science, Zhengzhou University, Zhengzhou 450001, China; 5 Wheat Research Institute, Sichuan Agricultural University, Chengdu 611130, China; 6 University of California at Davis, Davis, CA 95616, USA
  • Received:2014-06-30 Revised:2014-12-19 Published:2015-02-12 Published online:2014-12-29
  • Contact: 刘志勇, E-mail: zhiyongliu@cau.edu.cn

摘要:

为了解河南省近年小麦品种的遗传基础,利用Illumina 90k iSelect SNP标记技术对豫麦34及该省2000—2013年审定的小麦品种共96个进行全基因组扫描,分析了其遗传多样性和遗传基础。结果表明,在所有SNP位点中,多态性比率为47.39% (38 661/81 587),多态性标记在基因组间分布呈现B>A>D。96个品种亲缘关系较近,两两遗传相似系数的平均值为0.719,变幅为0.552~0.998,且94.3%的品种间遗传相似系数在0.652~0.812之间;按UPGMA法将96个品种划分为7个类群。综合SNP和系谱分析,近10年河南省审定的96个小麦品种遗传多样性不够丰富,多数品种亲缘关系较近,在育种中迫切需要引入新的种质资源,拓宽遗传背景。

关键词: 河南省, 小麦品种, 遗传多样性, SNP

Abstract:

To understand the genetic diversity of wheat varieties in Henan Province at whole genome level, we selected 96 registered wheat varieties in the past decade for SNP genotyping using the Illumina 90k iSelect SNP chip. The results showed that 47.39% of the SNPs (38 661 out of 81 587) were polymorphism between varieties. The genome distribution of polymorphic SNPs showed a tendency of B > A > D. The genetic similarity of the 96 varieties ranged from 0.552 to 0.998 with an average value of 0.719. However, genetic similarity of 0.652 to 0.812 was found for 94.3% of the tested varieties, indicating highly similarity for most of the varieties in Henan province. The 96 wheat varieties could be classified as seven groups by UPGMA analyses. Based on pedigree and SNP analyses, very narrow genetic background was observed for the registered varieties in the past decade in Henan province. It is an urgent task for wheat breeders to explore and introduce diversified germplasm resources into wheat breeding program to increase the yield potential and stress tolerance in the future.

Key words: Henan province, Wheat varieties, Genetic Diversity, SNPs

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