作物学报 ›› 2015, Vol. 41 ›› Issue (02): 207-213.doi: 10.3724/SP.J.1006.2015.00207
王秀娟,陈新宏,庞玉辉,敬樊,张军,胡思远,昝凯,武军,杨群慧,赵继新*
WANG Xiu-Juan,CHEN Xin-Hong,PANG Yu-Hui,JING Fan,ZHANG Jun,HU Si-Yuan,ZAN Kai,WU Jun,YANG Qun-Hui,ZHAO Ji-Xin *
摘要:
利用分子标记、细胞学、基因组原位杂交(GISH)等技术,结合田间农艺性状调察,对小麦–华山新麦草七倍体材料H8911与硬粒小麦D4286杂交F4代分离群体中杂交后代DH2322进行了综合鉴定。华山新麦草基因组特异SCAR标记鉴定表明,DH2322含有华山新麦草特异遗传物质;细胞遗传学观察显示,DH2322染色体构型为2n=42=21 II;有丝分裂和花粉母细胞减数分裂中期I基因组原位杂交(GISH)鉴定表明,DH2322的染色体由40条小麦染色体和2条华山新麦草Ns染色体构成,且两条Ns染色体能完全配对为一个二价体;SSR和STS分子标记分析表明,DH2322缺失了小麦D基因组的2D染色体,而含有华山新麦草的2Ns染色体;农艺性状调查分析表明,DH2322具有双亲的形态学特征,结实性好,穗长和穗粒数显著大于亲本。
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