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作物学报 ›› 2015, Vol. 41 ›› Issue (02): 207-213.doi: 10.3724/SP.J.1006.2015.00207

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦–华山新麦草异代换系DH2322的分子细胞遗传学鉴定

王秀娟,陈新宏,庞玉辉,敬樊,张军,胡思远,昝凯,武军,杨群慧,赵继新*   

  1. 西北农林科技大学农学院,陕西杨凌 712100
  • 收稿日期:2014-06-09 修回日期:2014-12-19 出版日期:2015-02-12 网络出版日期:2014-12-29
  • 通讯作者: 赵继新, E-mail: zhjx881@163.com
  • 基金资助:

    本研究由引进国际先进农业科学技术计划(948计划)项目(2013-Z28), 陕西省自然科学基础研究计划重点项目(2013JZ007)和西北农林科技大学唐仲英育种基金资助。

Molecular Cytogenetic Characterization of Triticum–Psathyrostachys Substitution Line DH2322

WANG Xiu-Juan,CHEN Xin-Hong,PANG Yu-Hui,JING Fan,ZHANG Jun,HU Si-Yuan,ZAN Kai,WU Jun,YANG Qun-Hui,ZHAO Ji-Xin *   

  1. College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China
  • Received:2014-06-09 Revised:2014-12-19 Published:2015-02-12 Published online:2014-12-29
  • Contact: 赵继新, E-mail: zhjx881@163.com

摘要:

利用分子标记、细胞学、基因组原位杂交(GISH)等技术,结合田间农艺性状调察,对小麦–华山新麦草七倍体材料H8911与硬粒小麦D4286杂交F4代分离群体中杂交后代DH2322进行了综合鉴定。华山新麦草基因组特异SCAR标记鉴定表明,DH2322含有华山新麦草特异遗传物质;细胞遗传学观察显示,DH2322染色体构型为2n=42=21 II;有丝分裂和花粉母细胞减数分裂中期I基因组原位杂交(GISH)鉴定表明,DH2322的染色体由40条小麦染色体和2条华山新麦草Ns染色体构成,且两条Ns染色体能完全配对为一个二价体;SSR和STS分子标记分析表明,DH2322缺失了小麦D基因组的2D染色体,而含有华山新麦草的2Ns染色体;农艺性状调查分析表明,DH2322具有双亲的形态学特征,结实性好,穗长和穗粒数显著大于亲本。

关键词: 小麦, 华山新麦草, 异代换系, SCAR, 基因组原位杂交(GISH), SSR, STS

Abstract:

A wheat–P. huashanica alien substitution line DH2322 was isolated from the F4 progeny of heptaploid hybrid H8911 × Triticum durum cv. D4286 and identified by using SCAR markers, genomic in situ hybridization (GISH) , SSR markers, and STS markers. The rusults using SCAR marker RHS141 showeded that DH2322 carrys chromosome derived from P. huashanica. Cytogenetic observation indicated that DH2322 has a chromosome karyotype of 2n = 42 = 21 II. GISH showed that DH2322 is a line with 40 chromosomes from wheat and two Ns chromosomes from P. huashanica, which formed a ring bivalent in PMC at metaphase I. Analysis with SSR and STS primers showed that the wheat chrom osome 2D in DH2322 was substituted by the chromosome 2Ns from P. huashanica. Evaluation of the agronomic traits showed that DH2322 presented favorable agronomic traits in spike length and kernels per spike. Development of this new germplasm will be useful for wheat breeding programs in the future.

Key words: Triticum aestivum, Psathyrostachys huashanica, Alien substitution line, SCAR, GISH, SSR, STS

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