作物学报 ›› 2015, Vol. 41 ›› Issue (04): 539-547.doi: 10.3724/SP.J.1006.2015.00539
赵兰杰1,薛飞2,朱守鸿2,李艳军2,刘永昌2*,孙杰2
ZHAO Lan-Jie1,XUE Fei2,ZHU Shou-Hong2,LI Yan-Jun2,LIU Yong-Chang2,*,SUN Jie2
摘要:
Kinesin家族是一类马达蛋白,它们能利用ATP水解所释放的能量驱动自身携带物质分子沿着微管运动,在细胞形成、细胞伸长等方面起着关键作用。本研究以拟南芥Atkinesin13A蛋白序列作为探针序列,利用Blast比对从二倍体雷蒙德氏棉的基因组数据库中发现7个具有较高同源关系的基因。根据基因序列设计引物,利用RT-PCR技术从陆地棉纤维中分离出7个基因。依据7个基因与Atkinesin13A和Atkinesin13B的同源性高低,依次将其命名为GhKIS13A1、GhKIS13A2、GhKIS13A3、GhKIS13B1、GhKIS13B2、GhKIS13B3和GhKIS13B4。生物信息学分析表明,7个Ghkinesin13均含有典型的KISC马达区域、ATP结合位点和微管结合位点,其马达区域属于中央马达。多重序列比对和进化树分析发现,这7个基因可被分为2个(Kinesin13A和Kinesin13B)亚类。实时荧光定量PCR结果表明,7个Ghkinesin13亚家族基因在棉花各组织中均有表达,但表达模式各不相同,其中GhKIS13B4在纤维中优势表达,表明其在纤维发育过程中可能发挥着重要作用。
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