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作物学报 ›› 2015, Vol. 41 ›› Issue (06): 845-860.doi: 10.3724/SP.J.1006.2015.00845

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

花生种子休眠解除过程中相关基因的表达分析

陈静1,4,江玲1,王春明1,胡晓辉4,翟虎渠3,万建民1,2,*   

  1. 1 南京农业大学作物遗传与种质创新国家重点实验室 / 江苏省植物基因工程技术研究中心, 江苏南京 210095; 2 中国农业科学院作物科学研究所, 北京 100081; 3 中国农业科学院, 北京 100081; 4 山东省花生研究所, 山东青岛 266100
  • 收稿日期:2014-12-15 修回日期:2015-03-09 出版日期:2015-06-12 网络出版日期:2015-04-21
  • 基金资助:

    本研究由山东省农业科学院青年英才培养计划,山东省良种工程(高产优质花生新品种培育),青岛民生计划(13-1-3-77-nsh), 青岛民生计划(14-2-3-34-nsh)资助。

Expression Analysis of Genes Involved in Peanut Seed Dormancy Release (Arachis hypogaea L.)

CHEN Jing1,4,JIANG Ling1,WANG Chun-Ming1,HU Xiao-Hui4,ZHAI Hu-Qu3,WAN Jian-Min1,2,*   

  1. 1 State Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Provincial Center of Plant Gene Engineering, Nanjing Agricultural University, Nanjing 210095, China; 2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 Chinese Academy of Agricultural Sciences, Beijing 100081, China; 4 Shandong Peanut Research Institute, Qingdao 266100, China
  • Received:2014-12-15 Revised:2015-03-09 Published:2015-06-12 Published online:2015-04-21

摘要:

种子休眠性是花生重要的农艺性状,外源乙烯利能诱导花生种子休眠的解除,为了阐明乙烯利作用下花生种子休眠解除的分子机制,设置吸胀的休眠种子为对照,100 mg L–1乙烯利处理吸胀休眠种子后不同时间的样品(AE1AE2AE3)进行转录组分析,比较了花生种子休眠解除过程中ABAGAETHauxin相关基因的表达。结果表明,15个与GA40个与ABA60个与ETH56个与auxin相关的unigenes在花生种子休眠解除过程中表现显著差异表达。荧光定量PCR结果显示,ABA合成关键基因AhNCED2和代谢关键基因AhCYP707A1在种子休眠解除过程中均受外源乙烯利诱导,表达差异显著;在休眠和无休眠种子吸胀萌发过程中,AhNCED2AhCYP707A1的表达趋势不同,AhNCED2对于种子休眠的维持发挥积极作用,而AhCYP707A1对于种子休眠解除发挥积极作用。

关键词: 花生, 休眠性, ABA, AhNCED2, AhCYP707A1

Abstract:

Seed dormancy is one of important agronomic traits in peanut (Arachis hypogaea L.). Seed dormancy can be released with exogenous ethephon. To understand the molecular mechanisms of switches from dormancy to germination in peanut seeds underlying the role of ethephon, we preformed transcriptome analyses among imbibed dormant seeds as control and dormancy-released seeds (AE1, AE2, AE3) treated by 100 mg L–1 exogenous ethephon, and compared the expression of unigenes related to ABA, GA, ETH and auxin. The results showed that there were 15, 40, 60, and 56 unigenes associated with GA, ABA, ETH, and auxin respectively, which were significantly differentially expressed unigenes during the process from dormancy to germination. The results of Real-time RT-PCR showed that the expressions of AhNCED2 and AhCYP707A1 were induced distinctly by exogenous ethephon in seed dormancy released process. In dormant and non-dormant seed imbibition and germination processes, there were different roles between expresses of AhNCED2 and AhCYP707A1. AhNCED2 played a positive role in maintaining seed dormancy, while AhCYP707A1 played a positive role for seed dormancy breaking.

Key words: Peanut, Seed, Dormancy, ABA, AhNCED2, AhCYP707A1

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