作物学报 ›› 2015, Vol. 41 ›› Issue (06): 861-871.doi: 10.3724/SP.J.1006.2015.00861
钟振泉**,罗文龙**,刘永柱,王慧,陈志强*,郭涛*
ZHONG Zhen-Quan**,LUO Wen-Long**,LIU Yong-Zhu,WANG Hui,CHEN Zhi-Qiang*,GUO Tao*
摘要:
从F2 (粤晶丝苗2号/H4) 群体中,鉴定出一份显性斑点叶突变体spl32 (spotted leaf 32)。其叶片褐色斑点受自然光诱导,在幼穗分化期从叶尖逐渐扩散至叶鞘,台盼蓝染色表明斑点并非由细胞死亡引起。以从F5杂合个体分离出的正常叶色植株为对照,斑点叶植株的穗粒数、结实率显著降低。斑点出现后,spl32的POD活性和MDA含量均显著高于对照;同时,spl32叶片光合色素含量降低,但荧光动力学参数并无显著变化。抽穗期人工接菌表明,spl32对水稻白叶枯病菌抗性较对照显著提高。遗传分析表明spl32斑点性状由一个显性基因Spl32(t)控制,利用F2 (02428/spl32)群体将其定位在第11染色体Ind-c和RM206之间,推测该基因为一个新的水稻斑点叶基因。
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