作物学报 ›› 2016, Vol. 42 ›› Issue (02): 222-229.doi: 10.3724/SP.J.1006.2016.00222
马富磊,李德谋,李志,杨卫娟,周雪,游宇,罗小英*
MA Fu-Lei,LI De-Mou,LI Zhi,YANG Wei-Juan,ZHOU Xue,YOU Yu,LUO Xiao-Ying*
摘要:
采用植物基因工程技术, 选用CaMV35S组成型启动子驱动棉花3-羟基-3-甲基-戊二酰辅酶A还原酶基因GhHMGR在棉花中表达, 检测10 DPA(days post anthesis)棉花胚珠内HMGR (3-hydroxy-3- methylglutaryl coenzyme A reductase)酶及可溶性糖、脂质及蛋白质含量, 同时进行了胚珠离体培养。结果显示, GhHMGR在棉花光照部位(叶柄和铃壳)表达量相对较高, 非光照部位(根及胚珠)表达量低;超量表达GhHMGR可部分恢复拟南芥hmgr突变体性状;相较野生型, 超量表达株系10 DPA胚珠的HMGR含量升高, 反义株系则降低;且超量表达株系总脂质及蛋白含量升高, 可溶性总糖含量降低, 反义株系则出现相反结果;HMGR竞争性抑制剂洛伐他汀处理会导致胚珠发育畸形。以上结果表明, GhHMGR基因在棉花胚珠生长发育过程中扮演着重要的角色。
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