作物学报 ›› 2016, Vol. 42 ›› Issue (02): 222-229.doi: 10.3724/SP.J.1006.2016.00222
马富磊,李德谋,李志,杨卫娟,周雪,游宇,罗小英*
MA Fu-Lei,LI De-Mou,LI Zhi,YANG Wei-Juan,ZHOU Xue,YOU Yu,LUO Xiao-Ying*
摘要:
采用植物基因工程技术, 选用CaMV35S组成型启动子驱动棉花3-羟基-3-甲基-戊二酰辅酶A还原酶基因GhHMGR在棉花中表达, 检测10 DPA(days post anthesis)棉花胚珠内HMGR (3-hydroxy-3- methylglutaryl coenzyme A reductase)酶及可溶性糖、脂质及蛋白质含量, 同时进行了胚珠离体培养。结果显示, GhHMGR在棉花光照部位(叶柄和铃壳)表达量相对较高, 非光照部位(根及胚珠)表达量低;超量表达GhHMGR可部分恢复拟南芥hmgr突变体性状;相较野生型, 超量表达株系10 DPA胚珠的HMGR含量升高, 反义株系则降低;且超量表达株系总脂质及蛋白含量升高, 可溶性总糖含量降低, 反义株系则出现相反结果;HMGR竞争性抑制剂洛伐他汀处理会导致胚珠发育畸形。以上结果表明, GhHMGR基因在棉花胚珠生长发育过程中扮演着重要的角色。
[1]杨红旗. 我国棉花生产现状与发展前景分析. 种子科技, 2010, 2: 5–6Yang H Q. The analysis of cotton production and development prospect in China. Seeds Sci Technol, 2010, 2: 5–6[2]Kim H J, Triplett B A. Cotton fiber growth in planta and in vitro Models for plant cell elongation and cell wall biogenesis. Plant Physiol, 2001, 127: 1361–1366[3]Bach T J. Some new aspects of isoprenoid biosynthesis in plants—a review. Lipids, 1995, 30: 191–202[4]Lombard J, Moreira D. Origins and early evolution of the mevalonate pathway of isoprenoid biosynthesis in the three domains of life. Mol Biol Evol, 2011, 28: 87–99[5]Wang Y, Darnay B G, Rodwell V W. Identification of the principal catalytically important acidic residues of 3-hydroxy-3-metIIylglutaryl coenzyme A reductase. J Biol Chem, 1990, 265: 21634–21641[6]陈大华, 叶和春, 李国凤, 刘彦. 植物类异戊二烯代谢途径的分子生物学研究进展. 植物学报, 2000, 42: 551–558Chen D H, Ye H C, Li G F. Cloning and sequencing of HMGR gene of Solanum tubersosum and its expression pattern. Acta Bot Sin, 2000, 42: 724–727 (in Chinese with English abstract)[7]Stermer B A, Bianchini G M, Korth K L. Regulation of HMG-CoA reductase activity in plants. J Lipid Res, 1994, 35: 1133–1140[8]Omura T, Watanabe S, Iijima Y, Aoki K, Shibata D, Ezura H. Molecular and genetic characterization of transgenic tomato expressing 3-hydroxy-3-methyl-glutaryl coenzyme A reductase. Plant Biotechnol, 2007, 24: 107–115[9]Luo M, Xiao Y H, Li X B, Lu X F, Deng W, Li D M, Hou L, Hu M Y, Li Y, Pei Y. GhDET2, a steroid 5α-reductase, play an important role in cotton fiber cell initiation and elongation. Plant J, 2007, 51: 419–430[10]Clough S J, Bent A F. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J, 1998, 16: 735–743[11]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem, 1976, 72: 248–254[12]Ge X, Wu J. Tanshinone production and isoprenoid pathways in Salvia miltiorrhiza hairy roots induced by Ag+ and yeast elicitor. Plant Sci, 2005, 168: 487–491 [13]Beasley C, Ting I P. The effects of plant growth substances on in vitro fiber development from fertilized cotton ovules. Am J Bot, 1973, 130–139[14]Beasley C, Ting I P. Effects of plant growth substances on in vitro fiber development from unfertilized cotton ovules. Am J Bot, 1974, 188–194[15]汤章城. 植物生理学指南. 上海: 中国科学出版社, 1999. p 1Tang Z C. The Guide of Modern Plant Physiology Experiment. Shanghai, Science Press, 1999. p 1[16]Xia R, Li C Q, Lu W J, Du J, Wang Z H, Li J G. 3-Hydroxy-3-methylglutaryl coenzyme A reductase 1 (HMG1 ) is highly associated with the cell division during the early stage of fruit development which determines the final fruit size in Litchi chinensis. Gene, 2012, 498: 28–35[17]Suzuki M, Kamide Y, Nagata N, Seki H, Ohyama K, Kato H, Masuda K, Sato S, Kato T, Tabata S, Yoshida S, Muranaka T. Loss of function of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (HMG1) in Arabidopsis leads to dwarfing, early senescence and male sterility, and reduced sterol levels. Plant J, 2004, 37: 750–761[18]Bach T J, Lichtenthaler H K. Mevinolin:a highly specific inhibitor of microsomal 3-hydroxy-3-methylglutaryl-coenzyme Areductase of radish plants. Z Naturforsch, 1982, 37: 46–50[19]Opitz S, Nes W D, Gershenzon J. Both methylerythritol phosphate and mevalonate pathways contribute to biosynthesis of each of the major isoprenoid classes in young cotton seedling. Phtochemistry, 2014, 98: 110–119 |
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