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作物学报 ›› 2016, Vol. 42 ›› Issue (02): 201-211.doi: 10.3724/SP.J.1006.2016.00201

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米SBP转录因子全基因组鉴定与功能分析

彭华1,**,何秀静2,**,高健4,罗茂3,潘光堂2,*,张志明2,*   

  1. 1 四川旅游学院,四川成都 610100;2 四川农业大学玉米研究所,四川温江 611130;3四川医科大学药物与功能性食品研究中心,四川泸州 646000;4第三军医大学西南医院病理学研究所西南癌症中心,重庆 400038
  • 收稿日期:2015-05-12 修回日期:2015-11-20 出版日期:2016-02-12 网络出版日期:2015-12-07
  • 通讯作者: 潘光堂, 张志明, E-mail: panlab605@gmail.com, Tel: 86-28-86290917
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2012AA10A307)和四川省科技厅青年基金项目(2015JQO021)资助。

Genome-wide Identification and Function Analysis of SBP Gene Family in Maize

PENG Hua1,**,HE Xiu-Jing2,**,GAO Jian4,LUO Mao3,PAN Guang-Tang2,*,ZHANG Zhi-Ming2,*   

  1. 1 SiChuan Tourism College, Chengdu 610100, China; Maize Research Institute of Sichuan Agricultural University, Wenjiang 611130, China; 3 Research Center for Drug Discovery of Luzhou Medical College, Luzhou 646000, China, 4 Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, and Key Laboratory of Tumor Immunopathology, Ministry of Education, Chongqing 400038, China
  • Received:2015-05-12 Revised:2015-11-20 Published:2016-02-12 Published online:2015-12-07
  • Contact: 潘光堂, 张志明, E-mail: panlab605@gmail.com, Tel: 86-28-86290917
  • Supported by:

    This study was supported by the National High Technology Research and Development Program of China (863 Program) (2012AA10A307) and Youth Science Fund Project from Technological Office of Sichuan Province (2015JQO021).

摘要:

SBP基因家族是一类植物基因组特有的转录因子,参与植物生长发育及多种生理生化过程。近来,大量研究已在多种植物中鉴定出SBP转录因子,但关于玉米(Zea mays L.) SBP转录因子家族的系统分析报道尚少。本研究通过对拟南芥、水稻等植物已知的转录因子与玉米基因组数据比对,并设置一系列严格的筛选标准从玉米基因组中挖掘SBP转录因子,系统发育分析显示单子叶植物玉米和水稻的SBP基因保守性更强、亲缘关系更近;共鉴定37个SBP基因,分布在9条染色体上;通过基因分析注释以及启动子功能预测,进一步发现SBP家族基因参与植物生长发育、形态建成、逆境胁迫响应、花器官发育以及植物光反应等过程。并且,玉米SBP转录因子可通过参与赤霉素、生长素、脱落酸、水杨酸等多条激素信号调控途径来调节植物的生长发育。

关键词: 玉米, SBP转录因子家族, 生物信息学

Abstract:

SBP gene family, as a plant special transcription factors is involved in plant growth and development, as well as many physiological and biochemical processes. Recently, SBP transcription factor family has been identified in model plants, such as Arabidopsis and Oryza sativa; however, systematic analysis of SBP transcription factor family in maize (Zea mays L.) is scarcely. In this study, based on homology alignment technology, we aligned all known SBP TFs from Arabidopsis and Oryza sativa with those from maize genome sequence to mine novel SBP TFs in maize. A total of 37 SBP TFs distributed in eight chromosomes were identified. Phylogenetic analysis indicated that SBP transcription factor genes have stronger homology, especially between Zea mays and Oryza sativa. Moreover, Promoters-cis Elements analysis of those SBP TFs demonstrated that they might be involved in plant growth and development, morphogenesis, adversity response, the development of flower organs and photosynthesis. It is probable that SBP TFs regulate plant growth and development through the multiple hormone of signaling transduction pathway, such as gibberellin, auxin, abscisic acid, and salicylic acid.

Key words: Maize, SBP TFs gene family, Bioinformatics

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