作物学报 ›› 2016, Vol. 42 ›› Issue (05): 641-647.doi: 10.3724/SP.J.1006.2016.00641
石璇1,王茹媛1,唐君2,李宗芸1,*,罗永海1,*
SHI Xuan1,WANG Ru-Yuan1,TANG Jun2,LI Zong-Yun1,*,LUO Yong-Hai1,*
摘要:
选用Xushu18 (6x)和Nancy Hall (6x)、2个二倍体I. trifida (2x)品系(4597-10和4597-21)、四倍体I. trifida (4x)、六倍体I. trifida (6x)、二倍体I. temussima (2x)和I. littorallis (2x)以简化基因组测序技术SLAF-seq测序,获得724 589个SLAF标签,其中多态性SLAF标签35 310个。通过序列分析,获得40 765个有效单核苷酸多态(SNP),并用这些SNP分析了8个种质的群体结构和系统发生树。结果表明,利用简化基因组测序技术SLAF-seq能高效、低成本地开发出大量可用于群体遗传分析的SNP标记;通过构建进化树发现甘薯栽培种和野生种I. trifida的亲缘关系比较近。这些分析结果为进一步研究甘薯栽培种的起源提供了基础数据。
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