作物学报 ›› 2017, Vol. 43 ›› Issue (05): 648-657.doi: 10.3724/SP.J.1006.2017.00648
韦荔全1,**,罗延敏1,**,王文强1,池长程1,黄福灯2,向珣1,程方民1,潘刚1,*
WEI Li-Quan1,**,LUO Yan-Min1,**,WANG Wen-Qiang1,CHI Chang-Cheng1,HUANG Fu-Deng2,XIANG Xun1,CHENG Fang-Min1,PAN Gang1,*
摘要:
通过EMS诱变籼稻恢复系珍97获得一个稳定遗传的褐色斑点叶突变体splZ97 (spotted leaf Z97, splZ97)。大田条件下,突变体splZ97的斑点叶性状始于分蘖期,此后由叶缘向叶中下部迅速扩散,直至整个叶片,严重时叶片部分或整体枯死,从而致使突变体株高、每穗粒数及结实率极显著低于野生型对照。生理分析表明,与野生型珍97相比,孕穗期突变体splZ97剑叶、倒二叶和倒三叶的叶绿素含量极显著降低,而POD (peroxidase, POD)活性、O2?含量及MDA (malondialdehyde, MDA)含量升高;突变体splZ97倒二叶和倒三叶的CAT (catalase, CAT)活性和可溶性蛋白含量极显著降低,而SOD (superoxide dismutase, SOD)活性则极显著增加。组织化学分析进一步证实,突变体splZ97的叶片明显累积O2?。此外,突变体splZ97苗期经盐胁迫处理后,其株高及根长明显受到抑制。遗传分析表明,突变体splZ97的斑点叶性状受一对隐性核基因控制,借助图位克隆技术将该基因定位于第12染色体长臂的RM28466与RM28485两个SSR标记之间,物理距离为189 kb,该结果为进一步克隆SPLZ97基因并研究其功能奠定了基础。
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