不同形态硼对油菜幼苗铝毒的缓解效应及其FTIR特征分析

doi:10.3724/SP.J.1006.2017.01817

摘要/Abstract

摘要：

采用水培法，以油菜品种Cao 221167为试验材料，设置无机态硼酸(BA)和有机态山梨醇硼(SB)及不同Al3+ (0、100、200和500 μmol L–1)处理，研究不同形态硼(B)对油菜幼苗铝(Al)毒的缓解作用及不同形态B之间的缓解效应差异，以及利用FTIR (傅里叶红外光谱)技术分析叶片各物质含量的变化。结果表明，Al毒胁迫下，不同形态B (BA和SB)处理，显著提高植株生物量和根系长度(0、100、200和500 μmol L–1 Al毒胁迫下BA处理根长分别增加了52.15%、101.45%、366.70%和18.73%；SB处理分别增加了46.80%、133.98%、261.36%和10.77%)，提高色素含量和SOD活性，而降低了Al含量、MDA含量和POD活性。不同形态B处理下，油菜幼苗在200 μmol L–1 Al3+处理下长势、生物量及色素含量最高。在500 μmol L–1 Al3+处理下，油菜幼苗株高、根长、总干鲜重及色素含量显著低于无Al处理；FTIR分析表明，Al毒胁迫下油菜叶片中蛋白质和低聚糖等含量明显上升，加B明显降低了蛋白质和低聚糖的含量，且BA处理降低幅度明显高于SB处理。说明不同形态B(BA和SB)的添加均明显缓解Al毒，且BA对Al毒缓解效果优于SB，这为农业生产中施用何种B肥来缓解Al毒起到一定的指导作用。

关键词: 油菜, 不同形态B, Al毒胁迫, FTIR, 缓解效应

Abstract:

An experiment was conducted in hydroponics using rape cultivar Cao 221167. Two treatment of B, inorganic borate (BA) and organic boron sorbitol (SB) along with different concentrations of Al3+ (0, 100, 200, and 500 μmol L–1). The effects of different forms of B on activities of antioxidant enzyme of leaves in rape seedlings were studied, and the changes of leaf component were analyzed by Fourier Transform Infrared Spectroscopy (FTIR) technique. Under Al toxicity, the addition of BA or SB could alleviate Al toxicity in plant, and significantly increase plant biomass and root length. The root length increased by 52.15%, 101.45%, 366.70%, and 18.73% in BA treatments and 46.80%, 133.98%, 261.36%, and 10.77% in SB treatments under 0, 100, 200, and 500 μmol L–1 Al3+ toxicity stress, respectively. B treatments increased pigment content and SOD activity and reduced contents of Al, MDA and POD activity. Under the addition of boron (BA or SB), the growth, biomass and pigment content of rape seedlings were higher in 200 μmol L–1 Al3+ treatment than in other treatments. In the 500 μmol L–1 Al3+ treatment, biomass and pigment content of rape seedling were significantly lower than those without Al application. The results from FTIR indicated that the contents of protein and oligosaccharide in leaves of rape seedling significantly increased under Al toxicity, however, significantly decreased when was added boron (BA or SB). In conclusion, the addition of different forms of B (BA or SB) could alleviate Al toxicity and the effect of BA is better than that of SB. The information provided by this study will facilitate alleviating Al toxicity in plant through applying a kind of B fertilizer in agricultural production.

Key words: Rape, Different forms of boron, Aluminum toxicity stress, FTIR, Mitigation effect

闫磊,姜存仓*,Muhammad Riaz,吴秀文,卢晓佩,杜晨晴,王宇函. 不同形态硼对油菜幼苗铝毒的缓解效应及其FTIR特征分析[J]. 作物学报, 2017, 43(12): 1817-1826.

YAN Lei, JIANG Cun-Cang*, Muhammad Riaz, WU Xiu-Wen, LU Xiao-Pei, DU Chen-Qing,WANG Yu-Han. Mitigative Effect of Different Forms of Boron on Aluminum Toxicity of Rape Seedlings and Its FTIR Characteristics[J]. Acta Agron Sin, 2017, 43(12): 1817-1826.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2017.01817

https://zwxb.chinacrops.org/CN/Y2017/V43/I12/1817

参考文献

[1] Delhaize E, Ryan P R. Aluminum toxicity and tolerance in plants. Plant Physiol, 1995, 107: 315–321 [2] Kochian L V. Cellular mechanisms of aluminum toxicity and resistance in plants. Annu Rev Plant Biol, 2003, 46: 237–260 [3] 沈仁芳. Al在土壤-植物中的行为及植物的适应机制. 北京: 科学出版社, 2008. pp 75–79 Shen R F. The Behavior of Aluminum in Soil Plants and the Adaptation Mechanism of Plants. Beijing: Science Press: 2008. 75–79 (in Chinese) [4] 肖厚军, 王正银, 何桂芳, 苟久兰. 贵州黄壤Al形态及其影响因素研究. 土壤通报, 2009, 40: 1044–1048 Xiao H J, Wang Z Y, He G F, Gou J L. Aluminum forms and their effect factors in yellow soils in Guizhou. Chin J Soil Sci, 2009, 40: 1044–1048 (in Chinese with English abstract) [5] 应小芳, 刘鹏, 徐根娣. 土壤中的Al及其植物效应的研究进展. 生态环境学报, 2003, 12: 237–239 Ying X F, Liu P, Xu G D. The advance in the research of aluminum in soil and its influence on plant. Eco Environ, 2003, 12: 237–239 (in Chinese with English abstract) [6] 刘鹏, 徐根娣, 姜雪梅, 应小芳. Al对大豆幼苗膜脂过氧化和体内保护系统的影响. 农业环境科学学报, 2004, 23: 51–54 Liu P, Xu G D, Jiang X M, Ying X F. Effects of aluminum on membrane lipid peroxidation and endogenous protective systems of soybean seedling. J Agro-Environ Sci, 2004, 23: 51–54 (in Chinese with English abstract) [7] 蔡妙珍, 刘鹏, 徐根娣, 吕庭君. 钙、硅对Al胁迫下荞麦光合生理的影响. 水土保持学报, 2008, 22(2): 206–208 Cai M Z, Liu P, Xu G D, Lyu T J. Effect of calcium and silicon on photosynthetic physiology of buckwheat under aluminum stress. J Soil Water Conserv, 2008, 22(2): 206–208 (in Chinese with English abstract) [8] 韩配配, 秦璐, 李银水, 廖祥生, 徐子先, 余常兵, 胡小加, 谢立华, 廖红. 不同营养元素缺乏对甘蓝型油菜苗期生长和根系形态的影响. 中国油料作物学报, 2016, 38: 88–97 Han P P, Qin L, Li Y S, Liao X S, Xu Z X, Yu C B, Hu X J, Xie L H, Liao H. Effects of different nutrient deficiencies on growth and root morphological changes of rapeseed seedlings (Brassica napus L.). Chin J Oil Crop Sci, 2016, 38: 88–97 (in Chinese with English abstract) [9] 杨国会, 王莉莹, 刘伟, 王一. 铝毒对大豆根系生长的影响. 安徽农业科学, 2009, 37: 1439–1440 Yang G H, Wang L Y, Liu W, Wang Y. Effects of aluminum toxicity on growth of soybean root. J Anhui Agric Sci, 2009, 37: 1439–1440 [10] 许苗苗, 喻敏, 王昌全, 徐彩娟, 萧洪东, 冯英明. 硼对豌豆根尖铝毒的影响. 土壤, 2007, 39(6): 154–157 Xu M M, Yu M, Wang C Q, Xu C J, Xiao H D, Feng Y M. Influence of boron on aluminum toxicity in root tip of pea (Pisum sativum). Soils, 2007, 39(6): 154–157 (in Chinese with English abstract) [11] Blevins D G, Lukaszewski K M. Boron in plant structure and function. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49: 481–500 [12] Lenoble M E, Blevins D G, Sharp R E, Cumbie B G. Prevention of aluminum toxicity with supplemental boron. I. Maintenance of root elongation and cellular structure. Plant Cell Environ, 1996, 19: 1132–1142 [13] 王志颖, 刘鹏, 李锦山, 吴慧芳, 刘莹, 芦伯鑫. 铝胁迫下外源有机酸对油菜根系形态及叶绿素荧光特性的影响. 江苏农业学报, 2011, 27: 756–762 Wang Z Y, Liu P, Li J S, Wu H F, Liu Y, Lu B X. Effects of exogenous organic acids on root morphology and chlorophyll fluorescence characteristics of oilseed rape under aluminum stress. Jiangsu J Agric Sci, 2011, 27: 756–762 (in Chinese with English abstract) [14] Yang Y H, Zhang H Y. Boron amelioration of aluminum toxicity in mungbean seedlings. J Plant Nutr, 1998, 21: 1045–1054 [15] Yu M, Shen R F, Xiao H D, Xu M M, Wang H Z, Wang H Y, Zeng Q L, Bian J F. Boron alleviates aluminum toxicity in pea (Pisum sativum). Plant Soil, 2009, 314: 87–98 [16] 徐建明, 汪鑫, 罗玉明, 李师默, 孙国荣, 陈刚. 两种形态硼对小麦幼苗叶绿素荧光参数保护酶活性的影响. 华北农学报, 2010, 24(2): 149–155 Xu J M, Wang X, Luo Y M, Li S M, Sun G R, Chen G. Effects of two forms of boron on antioxidant enzymes and chlorophyll fluorescence parameters of wheat seedlings. Acta Agric Boreali-Sin, 2010, 24(2): 149–155 (in Chinese with English abstract) [17] 段蔚. 多元醇络合硼对油菜幼苗生长及营养元素吸收的功效. 南京农业大学硕士学位论文, 江苏南京, 2012 Duan W. Effects of Polyol-Chelated Boron Fertilizers on Seedling growth and Uptake of Mineral Nutrients in Rape. MS Thesis of Nanjing Agricultural University, Nanjing, China, 2012 (in Chinese with English abstract) [18] 薛生国, 朱锋, 叶晟, 王钧, 吴雪娥. 紫茉莉对铅胁迫生理响应的FTIR研究. 生态学报, 2011, 31: 6143–6148 Xue S G, Zhu F, Ye C, Wang J, Wu X E. Physiological response of Mirabilis jalapa Linn. to lead stress by FTIR spectroscopy. Acta Ecol Sin, 2011, 31: 143–6148 (in Chinese with English abstract) [19] 龚宁, 李荣华, 孟昭福, 杨公明. Cd对小白菜萌发生理影响的FTIR-ATR研究. 农业环境科学学报, 2010, 29(1): 9–14 Gong N, Li R H, Meng Z F, Yang G M. Physiological response of Brassica chinensis L. seeds in germination to cadmium toxicity by FTIR-ATR pectroscopy. J Agro-Environ Sci, 2010, 29(1): 9–14 (in Chinese with English abstract) [20] 金婷婷, 刘鹏, 张志祥, 徐根娣, 赵莉莉. 外源柠檬酸缓解大豆根系短期Al胁迫的FTIR特征分析光谱学与光谱分析. 光谱学与光谱分析, 2009, 29: 367–371 Jin T T, Liu P, Zhang Z X, Xu G D, Zhao L L. Analysis of roots of soybean (Glycine max Merrill) treated with exogenous citric acid plus short-time aluminum stress by direct determination of FTIR spectrum. Spectrosc Spectral Anal, 2009, 29: 367–371 (in Chinese with English abstract) [21] 张子龙, 李加纳, 唐章林, 谌利, 王瑞. 环境条件对油菜品质的调控研究. 中国农学通报, 2006, 22(2): 124–124 Zhang Z L, Li J N, Tang Z L, Chen L, Wang R. The research progress of the effect of environmental factors on quality characters of rapeseed. Chin Agric Sci Bull, 2006, 22(2): 124–124 (in Chinese with English abstract) [22] 余礼明, 吴谋成. 油菜籽脱壳与分离设备研究注. 中国粮油学报, 2002, 17(5): 40–43 Yu L M, Wu M C. Developing equipment for dehulling and separation of rapeseed. J Chin Cereals Oils Assoc, 2002, 17(5): 40–43 (in Chinese with English abstract) [23] 王学奎. 植物生理生化实验原理和技术(第2版). 北京: 高等教育出版社, 2006. pp 167–280 Wang X K. Principles and Techniques of Plant Physiological Biochemical Experiment, 2nd edn. Beijing: Higher Education Press, 2006. pp 167–280 (in Chinese) [24] 鲍士旦. 土壤农化分析(第3版). 北京: 中国农业出版社, 2000. pp 276–278 Bao S D. Soil and Agricultural Chemistry Analysis, 3rd edn. Beijing: China Agriculture Press, 2000. pp 276–278 (in Chinese) [25] 攸玉仙. 植物样品中Al的测定. 物探与化探, 1997, 21:475–477 You Y X. Determining aluminum of plant specimen. Geophys Geochem Expl, 1997, 21: 475–477 (in Chinese with English abstract) [26] Delhaize E. Uptake on environmental stress: Aluminum toxicity and tolerance in plants. Plant Physiol, 1995, 107: 315–321 [27] 徐芬芬. 柠檬酸对Al胁迫下大豆根系生长和生理特性的影响. 生物加工过程, 2015, (4): 75–78 Xu F F. Effects of citric acid on root growth and physiological characteristics of soybean under aluminum stress. Chin J Bioprocess Eng, 2015, (4): 75–78 (in Chinese with English abstract) [28] 张芬琴, 徐新建. 外源硼对Al胁迫小麦幼苗的缓解效应. 植物生理学通讯, 2001, 37(1): 21–24 Zhang F Q, Xu X J. Mitigative effect of exogenous boron on the wheat seedlings under aluminum stress. Plant Physiol Communications, 2001, 37(1): 21–24 (in Chinese with English abstract) [29] Yang Y H, Gu H J, Fan W Y, Abdullahi B A. Effects of boron on aluminum toxicity on seedlings of two soybean cultivars. Water Air Soil Pollution, 2004, 154: 239–248 [30] Heidarabadi M D, Ghanati F, Fujiwara T. Interaction between boron and aluminum and their effects on phenolic metabolism of Linum usitatissimum L. roots. Plant Physiol Biochem, 2011, 49: 1377–1383 [31] 李梅, 喻敏. 硼对果胶Al吸附解吸特性的影响. 中国农业科学, 2013, 46: 1595–1602 Li M, Yu M. Characteristics of adsorption and desorption of aluminum in pectin as influenced by boron. Sci Agric Sin, 2013, 46: 1595–1602 (in Chinese with English abstract) [32] 王成, 蒋泽平, 李文青, 邵文静, 张婷, 季峻峰. FTIR-NIR研究镉胁迫对构树试管幼苗生长的影响与机理. 农业环境科学学报, 2014, 33: 673–679 Wang C, Jiang Z P, Li W Q, Shao E J, Zhang T, Ji J F. FTIR-NIR spectrum study on response and mechanism of Broussonetia papyrifera sprout under cadmium stresses. J Agro-Environ Sci, 2014, 33: 673–679 (in Chinese with English abstract) [33] 张亚楠, 王赢, 陈奇, 黄庶识, 李昆志. 不同品种荞麦耐Al性的FTIR鉴别. 浙江大学学报(农业与生命科学版), 2010, 36: 683–690 Zhang Y N. Wang Y, Chen Q, Huang S S, Li K Z. Endurance determination of different buckwheat cultivars to aluminum by FTIR spectrum. J Zhejiang Univ (Agric Life Sci Edn), 2010, 36: 683–690 (in Chinese with English abstract) [34] Snowden K C, Richards K D, Gardner R C. Aluminum induced genes induction by toxic metals, low-calcium, and wounding and pattern of expression in root tips. Plant Physiol, 1995, 107: 341–348 [35] Yang Q S, Wang Y Q, Zhang J J. Identification of aluminum-responsive proteins in rice roots by a proteomic approach: cysteine synthase as a key player in Al response. Proteomics, 2007, 7: 737 [36] 张争艳, 刘鹏, 陈微微, 陈传奇, 贾佳. 不同品种大豆耐Al性的FTIR分析研究. 光谱学与光谱分析, 2009, 29: 372–377 Zhang Z Y, Liu P, Chen W W, Chen C Q, Jia J. FTIR study of the endurance to al of different soybean cultivars. Spectrosc Spectral Anal, 2009, 29: 372–377 (in Chinese with English abstract)

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