欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2023, Vol. 49 ›› Issue (6): 1690-1698.doi: 10.3724/SP.J.1006.2023.22027

• 耕作栽培·生理生化 • 上一篇    下一篇

生长素调控水稻颖花开放的效应研究

何永明(), 张芳*()   

  1. 江西农业大学/作物生理生态与遗传育种教育部重点实验室/江西省作物生理生态与遗传育种重点实验室, 江西南昌 330045
  • 收稿日期:2022-05-07 接受日期:2022-10-10 出版日期:2023-06-12 网络出版日期:2022-10-26
  • 通讯作者: *张芳, E-mail: zhangf0124@126.com
  • 作者简介:E-mail: hymcom@126.com
  • 基金资助:
    国家自然科学基金项目(31360295);国家自然科学基金项目(31801272);江西省自然科学基金项目(20212BAB205006);江西省自然科学基金项目(20202BABL215001)

Study of regulating effect of auxin on floret opening in rice

HE Yong-Ming(), ZHANG Fang*()   

  1. Jiangxi Agricultural University/Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education/Jiangxi Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Nanchang 330045, Jiangxi, China
  • Received:2022-05-07 Accepted:2022-10-10 Published:2023-06-12 Published online:2022-10-26
  • Contact: *E-mail: zhangf0124@126.com
  • Supported by:
    National Natural Science Foundation of China(31360295);National Natural Science Foundation of China(31801272);Jiangxi Provincial Natural Science Foundation(20212BAB205006);Jiangxi Provincial Natural Science Foundation(20202BABL215001)

RichHTML

23

PDF

376

摘要：

颖花开放由浆片膨大所启动, 对水稻授粉受精具有直接影响。生长素是调节花药开裂、花粉育性和种子起始等生殖发育过程的重要激素。为阐明生长素在水稻颖花开放中的调控作用, 本研究以粳稻品种中花11为试验材料, 调查了外源生长素及其抑制剂对颖花开放的影响, 以及内源生长素水平和生长素信号通路基因表达的动态变化。结果表明, IAA (10~20 mmol L-1)、NAA (0.05~0.50 mmol L-1)浸穗处理将推迟水稻颖花开放, 其中高浓度(0.5 mmol L-1) NAA能使颖花开放推迟3 d, 并表现出颖花张开时间延长和结实率下降的现象。IAA极性运输抑制剂TIBA及其作用抑制剂PCIB也抑制颖花开放。NAA预处理后, 增施茉莉酸甲酯(MeJA)能有效恢复颖花开放。水稻颖花中IAA含量在自然开放前2 h迅速下降, 比开颖前1 d、2 d分别降低了65.85%、74.27%。与IAA水平变化相对应, 颖花开放时浆片IAA生物合成基因(OsTAR2、OsYUCCA3/4/8)表达下调, 而催化IAA结合失活的酶基因(OsGH3.2、OsGH3.8)、IAA输出载体基因(OsPIN1、OsPIN1a)以及IAA极性运输正向调节因子BG1基因均显著上调表达。此外, 我们也鉴定到13个差异表达的IAA早期响应基因(OsAUX/IAAs、OsARF15、OsSAURs), 其中10个上调表达, 3个下调表达。综上表明, 水稻颖花开放受内源生长素调控, 但提高浆片生长素水平则抑制其开放。

关键词: 水稻, 颖花开放, 生长素, 抑制效应

Abstract:

Floret opening which is driven by swelling of lodicules has a direct effect on rice pollination and fertilization. Auxin is an essential phytohormone in regulating reproductive development processes such as anther dehiscence, pollen fertility, and seed initiation. To elucidate the role of auxin in floret opening, the effects of exogenous auxin and its inhibitors on floret opening, and dynamic changes of endogenous auxin levels, and gene relative expression levels of auxin pathway in florets and lodicules were investigated in japonica cultivar Zhonghua 11. The results showed that the panicles soaked with IAA (10-20 mmol L-1) or NAA (0.05-0.50 mmol L-1) delayed significantly floret opening. Under high concentration of NAA (0.5 mmol L-1), compared with the water-treated panicles, the floret opening was postponed by three days. The prolonged opening duration of single floret and decreased seed-setting rate were also observed after IAA and NAA treatments. Treatments with IAA polar transport inhibitor TIBA and function inhibitor PCIB delayed florets opening as well. Furthermore, the application of methyl jamonate (MeJA) could restore effectively floret opening which was retared by NAA pretreatments. A sharp decline of IAA levels was detected two hours before floret opening in natural condition. Compared to that at 1 d and 2 d before opening, IAA level in florets at 2 hours before opening was decreased by 65.85% and 74.27%, respectively. Corresponding to the changes of IAA levels in florets, the relative expression levels of IAA biosynthetic genes (OsTAR2, OsYUCCA3/4/8) in the lodicules were down-regulated during floret opening, while the expressions of catabolic genes (OsGH3.2/OsGH3.8) in formation of inactive IAA conjugates, IAA efflux transport genes (OsPIN1, OsPIN1a), and its positive regulator BG1 gene were significantly up-regulated. 13 differentially expressed early auxin response genes (OsAUX/IAAs, OsARF15, OsSAURs) were also identified, among which, 10 were up-regulated and 3 down-regulated. In conclusion, rice floret opening was regulated by endogenous auxin, but was inhibited by elevating auxin level in lodicules.

Key words: rice, floret opening, auxin, inhibitory effect

图1

不同生长素处理下的水稻颖花开放动态 A: 不同生长素处理后4 d内水稻颖花开放情况。B: 不同生长素处理后水稻颖花开放最多的当天逐时开花情况。C: 不同生长素处理后单朵颖花开闭历时。不同小写字母代表在0.05概率水平差异显著(Duncan’s检验)。"

图2

不同生长素处理下的单穗自交结实率 不同小写字母代表在0.05概率水平差异显著(Duncan’s检验)。"

图3

TIBA (A)和PCIB (B)处理下水稻颖花的开放动态"

图4

MeJA对生长素抑制水稻颖花开放的影响"

图5

水稻颖花开放前不同时间的IAA含量 BF2d、BF1d、BF2h和BF0h分别表示开花前2 d、1 d、2 h和颖花开放时。不同小写字母代表在0.05概率水平差异显著(Duncan’s检验)。"

图6

浆片中IAA信号通路相关基因的表达分析 A: 水稻颖花开放前1 d和颖花开放时浆片转录组分析。B: OsYUCAA3/4/5/7/8, OsGH3.2/3.8, OsPIN1/1a和BG1等10个选择基因的表达变化值qRT-PCR检测与RNA-Seq的相关性分析。横坐标为qRT-PCR的相对表达水平, 纵坐标为RNA-Seq的差异表达水平, 二者均取log2的对数。BF1d和BF0h分别表示开花前1 d和颖花开放时。"

[1] 宋昕蔚, 林建荣, 吴明国. 水稻籼粳亚种间杂种优势利用研究进展与展望. 科学通报, 2016, 61: 3778-3786.
Song X W, Lin J R, Wu M G. Review and prospect on utilization of heterosis between indica-japonica rice subspecies. Chin Sci Bull, 2016, 61: 3778-3786. (in Chinese with English abstract)
doi: 10.1360/N972016-01039
[2] 徐伟东, 蔡金洋, 杨尧城. 水稻籼粳亚种间杂种优势利用研究现状与展望. 中国稻米, 2016, 22(2): 1-7.
doi: 10.3969/j.issn.1006-8082.2016.02.001
Xu W D, Cai J Y, Yang Y C. Research progress and prospect on utilization of heterosis between indica-japonica rice subspecies. China Rice, 2016, 22(2): 1-7. (in Chinese with English abstract)
[3] 王胜军, 王广峰, 范俊山, 张春和, 闫双勇, 童继平, 马忠友, 苏京平, 孙林静, 刘学军. 粳型三系杂交稻亲本开花习性研究. 贵州农业科学, 2009, 37(12): 12-14.
Wang S J, Wang G F, Fan J S, Zhang C H, Yan S Y, Tong J P, Ma Z Y, Su J P, Sun L J, Liu X J. Study on flowering habit of sterile and restorer lines of hybrid japonica rice. Guizhou Agric Sci, 2009, 37(12): 12-14. (in Chinese with English abstract)
[4] 林建荣, 宋昕蔚, 吴明国, 程式华. 籼粳超级杂交稻育种技术创新与品种培育. 中国农业科学, 2016, 49: 207-218.
doi: 10.3864/j.issn.0578-1752.2016.02.002
Lin J R, Song X W, Wu M G, Cheng S H. Breeding technology innovation of indica-japonica super hybrid rice and varietal breeding. Sci Agric Sin, 2016, 49: 207-218. (in Chinese with English abstract)
[5] 何永明, 曾晓春, 向妙莲, 黄俊宝, 付永琦. 水稻花时调控研究进展. 湖北农业科学, 2014, 53: 1489-1492.
He Y M, Zeng X C, Xiang M L, Huang J B, Fu Y Q. Advances on floret opening time of rice. Hubei Agric Sci, 2014, 53: 1489-1492. (in Chinese with English abstract)
[6] 张萌, 戴冬青, 李西明, 张华丽, 马良勇. 水稻花时性状研究进展. 核农学报, 2016, 30: 267-274.
doi: 10.11869/j.issn.100-8551.2016.02.0267
Zhang M, Dai D Q, Li X M, Zhang H L, Ma L Y. Advances on the study of flowering time trait in hybrid rice. J Nuclear Agric Sci, 2016, 30: 267-274. (in Chinese with English abstract)
[7] 黄友明, 曾晓春. 多功能调节剂对杂交水稻制种母本颖花开闭和种子生产的影响. 杂交水稻, 2022, 37(3): 87-94.
Huang Y M, Zeng X C. Effects of multifunctional regulators on spikelet opening and closing and seed production of the female parent in hybrid rice seed production. Hybrid Rice, 2022, 37(3): 87-94. (in Chinese with English abstract)
[8] 曾晓春, 周燮. 茉莉酸甲酯(MeJA)诱导水稻颖花开放. 植物学报, 1999, 41: 560-562.
Zeng X C, Zhou X. Methyl jasmonate induces the opening of spikelets in rice. Acta Bot Sin, 1999, 41: 560-562. (in Chinese with English abstract)
[9] 何永明, 林拥军, 曾晓春. 水稻颖花自然开放过程中茉莉酸(JA)生物合成的变化. 作物学报, 2012, 38: 1891-1899.
doi: 10.3724/SP.J.1006.2012.01891
He Y M, Lin Y J, Zeng X C. Dynamic changes of jasmonic acid biosynthesis in rice florets during natural anthesis. Acta Agron Sin, 2012, 38: 1891-1899. (in Chinese with English abstract)
doi: 10.3724/SP.J.1006.2012.01891
[10] 宋平, 夏凯, 吴传万, 包冬萍, 陈丽莉, 周燮, 曹显祖. 雄性不育和可育水稻开颖对茉莉酸甲酯响应的差异. 植物学报, 2001, 43: 480-485.
Song P, Xia K, Wu C W, Bao D P, Chen L L, Zhou X, Cao X Z. Differential response of floret opening in male-sterile and male-fertile rices to methyl jasmonate. Acta Bot Sin, 2001, 43: 480-485. (in Chinese with English abstract)
[11] 黄俊宝, 何永明, 曾晓春, 向妙莲, 付永琦. 水稻颖花开放前花器官茉莉酸水平变化及浆片茉莉酸信号基因表达分析. 中国农业科学, 2015, 48: 1219-1227.
doi: 10.3864/j.issn.0578-1752.2015.06.17
Huang J B, He Y M, Zeng X C, Xiang M L, Fu Y Q. Changes of JA levels in floral organs and expression analysis of JA signaling genes in lodicules before floret opening in rice. Sci Agric Sin, 2015, 48: 1219-1227. (in Chinese with English abstract)
[12] 付永琦, 向妙莲, 蒋海燕, 何永明, 曾晓春. 水稻颖花开放前浆片转录组变化. 中国农业科学, 2016, 49: 1017-1033.
doi: 10.3864/j.issn.0578-1752.2016.06.001
Fu Y Q, Xiang M L, Jiang H Y, He Y M, Zeng X C. Transcriptome profiling of lodicules before floret opening in Oryza sativa L. Sci Agric Sin, 2016, 49: 1017-1033. (in Chinese with English abstract)
[13] Biswas K K, Neumann R, Haga K, Yatoh O, Iino M. Photomorphogenesis of rice seedlings: a mutant impaired in phytochrome-mediated inhibition of coleoptile growth. Plant Cell Physiol, 2013, 44: 242-254.
doi: 10.1093/pcp/pcg040
[14] 潘孝武, 刘文强, 黎用朝, 熊海波, 盛新年, 段永红, 余亚莹, 赵文锦, 魏秀彩, 李小湘. 水稻裂颖突变体sh1的鉴定及基因定位. 中国水稻科学, 2019, 33: 323-330.
doi: 10.16819/j.1001-7216.2019.9027
Pan X W, Liu W Q, Li Y C, Xiong H B, Sheng X N, Duan Y H, Yu Y Y, Zhao W J, Wei X C, Li X X. Identification and genetic analysis of split husk mutant sh1 in rice. Chin J Rice Sci, 2019, 33: 323-330. (in Chinese with English abstract)
[15] Liao L, Shi C H, Zeng D D, Jin X L, Wu J G. Morphogenesis and molecular basis on the unclosed glumes, a novel mutation related to the floral organ of rice. Plant Mol Biol Rep, 2015, 33: 480-489.
doi: 10.1007/s11105-014-0764-7
[16] Li X H, Wang Y H, Duan E C, Qi Q, Zhou K N, Lin Q Y, Wang D, Wang Y L, Long W H, Zhao Z G, Cheng Z J, Lei C L, Zhang X, Guo X P, Wang J L, Wu C Y, Jiang L, Wang C M, Wan J M. OPEN GLUME1: a key enzyme reducing the precursor of JA, participates in carbohydrate transport of lodicules during anthesis in rice. Plant Cell Rep, 2018, 37: 329-346.
doi: 10.1007/s00299-017-2232-y pmid: 29177846
[17] Liu L, Zou Z S, Qian K, Xia C, He Y, Zeng H L, Zhou X, Riemann M, Yin C X. Jasmonic acid deficiency leads to scattered floret opening time in cytoplasmic male sterile rice Zhenshan 97A. J Exp Bot, 2017, 68: 4613-4625.
doi: 10.1093/jxb/erx251 pmid: 28981770
[18] 林俊城, 田小海, 殷桂香, 汤吉洪, 杨志刚. 人工调节籼型杂交水稻不育系花时的研究. 中国农业科学, 2008, 41: 2474-2479.
Lin J C, Tian X H, Yin G X, Tang J H, Yang Z G. Artificial regulation of the flowering time of CMS lines in indica hybrid rice seed production. Sci Agric Sin, 2008, 41: 2474-2479. (in Chinese with English abstract)
[19] 闫志强, 徐海, 马作斌, 高东昌, 徐正进. 籼稻与粳稻花时对茉莉酸甲酯(MeJA)响应的敏感性差异. 中国农业科学, 2014, 47: 2529-2540.
doi: 10.3864/j.issn.0578-1752.2014.13.005
Yan Z Q, Xu H, Ma Z B, Gao D C, Xu Z J. Differential response of floret opening to exo-methyl jasmonate between subsp. indica and subsp. japonica in rice. Sci Agric Sin, 2014, 47: 2529-2540. (in Chinese with English abstract)
[20] 王依明, 王冬翼, 顾春军, 吴雪源, 戴国忠. 茉莉酸甲酯喷施时间对粳型水稻不育系花时诱导效应研究. 上海农业学报, 2015, 31(6): 91-94.
Wang Y M, Wang D X, Gu C J, Wu X Y, Dai G Z. Effect of methyl jasmonate spraying time on keng rice sterile lines’ flowering habit. Acta Agric Shanghai, 2015, 31(6): 91-94. (in Chinese with English abstract)
[21] Huang Y M, Zeng X C, Cao H P. Hormonal regulation of floret closure of rice (Oryza sativa). PLoS One, 2018, 13: e0198828.
[22] Sazuka T, Kamiya N, Nishimura T, Ohmae K, Sato Y, Imamura K, Nagato Y, Koshiba T, Nagamura Y, Ashikari M. A rice tryptophan deficient dwarf mutant, tdd1, contains a reduced level of indole acetic acid and develops abnormal flowers and organless embryos. Plant J, 2009, 60: 227-241.
doi: 10.1111/tpj.2009.60.issue-2
[23] Zhao Z G, Zhang Y H, Liu X, Zhang X, Liu S C, Yu X W, Ren Y L, Zheng X M, Zhou K N, Jiang L, Guo X P, Gai Y, Wu C Y, Zhai H Q, Wang H Y, Wan J M. A role for a dioxygenase in auxin metabolism and reproductive development in rice. Dev Cell, 2013, 27: 113-122.
doi: 10.1016/j.devcel.2013.09.005 pmid: 24094741
[24] Wu H M, Xie D J, Tang Z S. PINOID regulates floral organ development by modulating auxin transport and interacts with MADS16 in rice. Plant Biotechnol J, 2020, 18: 1778-1795.
doi: 10.1111/pbi.v18.8
[25] Liu H B, Li X H, Xiao J H, Wang S P. A convenient method for simultaneous quantification of multiple phytohormones and metabolites: application in study of rice-bacterium interaction. Plant Methods, 2012, 8: 2.
doi: 10.1186/1746-4811-8-2 pmid: 22243810
[26] 张芳, 陆涵, 何永明. 水稻雄蕊发育晚期赤霉素生物合成特性分析. 江西农业大学学报, 2022, 44(1): 21-28.
Zhang F, Lu H, He Y M. Characteristics of gibberellin biosynthesis in late stamen development of rice. Acta Agric Univ Jiangxiensis, 2022, 44(1): 21-28. (in Chinese with English abstract)
[27] 李金涛, 樊海燕, 赵琦琦, 逯丹阳, 杨玉娜, 易庆平, 刘明艳, 贾玉芳, 彭波, 简清梅. 脱落酸对水稻根系生长素合成与运输的调控. 信阳师范学院学报(自然科学版), 2019, 32(1): 39-46.
Li J T, Fan H Y, Zhao Q Q, Lu D Y, Yang Y N, Yi Q P, Liu M Y, Jia Y F, Pen B, Jian Q M. Research of abscisic acid modulated auxin biosynthesis and transport in rice root. J Xinyang Norm Univ (Nat Sci Edn), 2019, 32(1): 39-46. (in Chinese with English abstract)
[28] Woo Y M, Park H J, Mukhamad S, Yang J L, Park J J, Back K, Park Y M, An G. Constitutively wilted 1, a member of the rice YUCCA gene family, is required for maintaining water homeostasis and an appropriate root to shoot ratio. Plant Mol Biol, 2007, 65: 126-136.
[29] Du H, Liu H B, Xiong L Z. Endogenous auxin and jasmonic acid levels are differentially modulated by abiotic stresses in rice. Front Plant Sci, 2013, 4: 389-397.
[30] Liu L C, Tong H N, Xiao Y H, Che R H, Xu F, Hu B, Liang C Z, Chu J F, Li J Y, Chu C C. Activation of big grain1 significantly improves grain size by regulating auxin transport in rice. Proc Natl Acad Sci USA, 2015, 112: 11102-11107.
doi: 10.1073/pnas.1512748112 pmid: 26283354
[31] Yin C X, Wu Q R, Zeng H L, Xia K, Xu J W, Li R W. Endogenous auxin is required but supraoptimal for rapid growth of rice (Oryza sativa L.) seminal roots, and auxin inhibition of rice seminal root growth is not caused by ethylene. J Plant Growth Regul, 2011, 30: 20-29.
doi: 10.1007/s00344-010-9162-z
[32] Wang Y D, Zhang T, Wang R C, Zhao Y D. Recent advances in auxin research in rice and their implications for crop improvement. J Exp Bot, 2018, 69: 255-263.
doi: 10.1093/jxb/erx228 pmid: 28992208
[33] 沈卫平, 蔡强, 周锋利, 张建中, 张大兵, 袁政. 植物激素调控水稻花器官发育分子机制的研究进展. 植物生理学报, 2015, 51: 593-600.
Shen W P, Cai Q, Zhou F L, Zhang J Z, Zhang D B, Yuan Z. Advances in the molecular mechanism underlying phytohormones function in regulating rice flower development. Plant Physiol J, 2015, 51: 593-600. (in Chinese with English abstract)
[34] van Doorn W G, Dole I, Celikel F G, Harkema H. Opening of Iris flowers is regulated by endogenous auxins. J Plant Physiol, 2013, 170: 161-164.
doi: 10.1016/j.jplph.2012.09.014
[35] Ke M, Gao Z, Chen J Q, Qiu Y T, Zhang L S, Chen X. Auxin controls circadian flower opening and closure in the waterlily. BMC Plant Biol, 2018, 18: 143.
doi: 10.1186/s12870-018-1357-7 pmid: 29996787
[36] Staswick P E. The tryptophan conjugates of jasmonic and indole-3-acetic acids are endogenous auxin inhibitors. Plant Physiol, 2009, 150: 1310-1321.
doi: 10.1104/pp.109.138529 pmid: 19458116
[37] Cecchetti V, Altamura M M, Brunetti P, Petrocelli V, Falasca G, Ljung K, Costantino P, Cardarelli M. Auxin controls Arabidopsis anther dehiscence by regulating endothecium lignification and jasmonic acid biosynthesis. Plant J, 2013, 74: 411-422.
doi: 10.1111/tpj.2013.74.issue-3
[38] Cai X T, Xu P, Zhao P X, Liu R, Yu L H, Xiang C B. Arabidopsis ERF109 mediates cross-talk between jasmonic acid and auxin biosynthesis during lateral root formation. Nat Commun, 2014, 5: 5833.
doi: 10.1038/ncomms6833
[39] Cao X, Yang H L, Shang C Q, Ma S, Liu L, Cheng J L. The roles of auxin biosynthesis in YUCCA gene family in plants. Int J Mol Sci, 2019, 20: 6343.
doi: 10.3390/ijms20246343
[40] Dhika A, Felix P F, Ulla N, Marine P, Jan Š, Zhang Y J, Chen Z L, Andrea G, Alisdair R F, Karin L, Iván F A. Auxin boosts energy generation pathways to fuel pollen maturation in barley. Curr Biol, 2022, 32: 1798-1811.
doi: 10.1016/j.cub.2022.02.073
[41] Zhang T, Li R N, Xing J L, Yan L, Wang R C, Zhao Y D. The YUCCA-Auxin-WOX11 module controls crown root development in rice. Front Plant Sci, 2018, 9: 523.
doi: 10.3389/fpls.2018.00523 pmid: 29740464
[42] Lee M, Jung J H, Han D Y, Seo P J, Park W J, Park C M. Activation of a flavin monooxygenase gene YUCCA7 enhances drought resistance in Arabidopsis. Planta, 2012, 235: 923-938.
doi: 10.1007/s00425-011-1552-3
[43] Staswick P E, Serban B, Rowe M, Tiryaki I, Maldonado M T, Maldonado M C, Suza W. Characterization of an arabidopsis enzyme family that conjugates amino acids toindole-3-acetic acid. Plant Cell, 2005, 17: 616-627.
doi: 10.1105/tpc.104.026690 pmid: 15659623
[44] 黄薇, 孙琦, 刘芳, 金玉环, 罗先梅, 黄先忠. 小拟南芥激素相关基因及GH3.6的克隆与表达. 石河子大学学报, 2019, 37: 323-331.
Huang W, Sun Q, Liu F, Jin Y H, Luo X M, Huang X Z. Analysis of hormone-related genes of Arabidopsis pumila and cloning and expression of GH3.6 gene. J Shihezi Univ, 2019, 37: 323-331. (in Chinese with English abstract)
[45] Ding X H, Cao Y L, Huang L L, Zhao J, Xu C G, Li X H, Wang S P. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate- and jasmonate-independent basal immunity in rice. Plant Cell, 2008, 20: 228-240.
doi: 10.1105/tpc.107.055657 pmid: 18192436
[46] Nathan L M, Ute V, Alexander W, George J, Duncan B, Anthony B, Malcolm J B, Markus G, Darren M W, Leah R B. Systems approaches reveal that ABCB and PIN proteins mediate co-dependent auxin efflux. Plant Cell, 2022, 34: 2309-2327.
doi: 10.1093/plcell/koac086
[47] Geisler M, Blakeslee J J, Bouchard R, Lee O R, Vincenzetti V, Bandyopadhyay A, Titapiwatanakun B, Peer W A, Bailly A, Richards E L. Cellular efflux of auxin catalyzed by the Arabidopsis MDR/PGP transporter AtPGP1. Plant J, 2005, 44: 179-194.
pmid: 16212599
[48] Bouchard R, Bailly A, Blakeslee J J, Oehring S C, Vincenzetti V, Lee O R, Paponov I, Palme K, Mancuso S, Murphy A S. Immunophilin-like TWISTED DWARF1 modulates auxin efflux activities of Arabidopsis P-glycoproteins. J Biol Chem, 2006, 281: 30603-30612.
doi: 10.1074/jbc.M604604200
[49] Cho M, Lee Z W, Cho H T. ATP-binding cassette B4, an auxin-efflux transporter, stably associates with the plasma membrane and shows distinctive intracellular trafficking from that of PIN-FORMED proteins. Plant Physiol, 2012, 159: 642-654.
doi: 10.1104/pp.112.196139 pmid: 22492845
[50] Kamimoto Y, Terasaka K, Hamamoto M, Takanashi K, Fukuda S, Shitan N, Sugiyama A, Suzuki H, Shibata D, Wang B. Arabidopsis ABCB21 is a facultative auxin importer/exporter regulated by cytoplasmic auxin concentration. Plant Cell Physiol, 2012, 53: 2090-2100.
doi: 10.1093/pcp/pcs149
[51] Xu Y X, Zhang S N, Guo H P, Wang S K, Xu L G, Li C Y, Qian Q, Chen F, Markus G, Qi Y H, Jiang D A. OsABCB14 functions in auxin transport and iron homeostasis in rice (Oryza sativa L.). Plant J, 2014, 79: 106-117.
doi: 10.1111/tpj.2014.79.issue-1
[1] 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[2] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[3] 张曦, 王广恩, 李邵琦, 刘祎, 李俊兰, 钱玉源. 基于转录组测序解析陆海杂交姊妹系马克隆值差异的形成机制[J]. 作物学报, 2026, 52(5): 1442-1458.
[4] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[5] 陈伟, 卫万娟, 赵其兵, 常东伟, 余凌波, 翟鹏飞, 冯志明, 陈宗祥, 任仰涛, 杨鹏, 刘海浪, 李珍富, 杨永乐, 金彦刚, 左示敏. 利用CRISPR/Cas9编辑Hd6基因创制优质早熟水稻新种质[J]. 作物学报, 2026, 52(4): 1046-1056.
[6] 石少阶, 刘凯, 陈姿夷, 王卉颖, 李三和, 周雷, 游艾青. 水稻矮化多分蘖基因DMT1的克隆与功能分析[J]. 作物学报, 2026, 52(4): 1022-1034.
[7] 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812.
[8] 叶凡, 李帅, 李思宇, 陈云, 窦超银, 刘立军. 不同节水灌溉方式对东北稻区水稻产量和群体质量的影响[J]. 作物学报, 2026, 52(3): 895-907.
[9] 王婵, 吴莹莹, 李文奇, 李霞, 王芳权, 周彤, 杨杰. 基于HRM技术开发水稻抗条纹叶枯病基因STV11功能标记[J]. 作物学报, 2025, 51(9): 2547-2556.
[10] 陈惠莹, 何嘉欣, 朱斌, 黄士轩, 周星佑, 伍君权, 杨美艳. 水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究[J]. 作物学报, 2025, 51(8): 2087-2099.
[11] 杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724.
[12] 雷松翰, 范骏扬, 车艳奕, 代永东, 郑雨萌, 田维江, 桑贤春, 王晓雯. 水稻内卷叶突变体acl3的鉴定及调控基因的功能分析[J]. 作物学报, 2025, 51(6): 1467-1479.
[13] 李福媛, 杨奕, 马继琼, 许明辉, 林良斌, 孙一丁. 水稻OsPUB4基因克隆、激素诱导表达分析与互作蛋白筛选[J]. 作物学报, 2025, 51(6): 1690-1700.
[14] 王梦宁, 谢可冉, 高逖, 王飞, 任孝俭, 熊栋梁, 黄见良, 彭少兵, 崔克辉. 水稻幼穗分化期至抽穗期高温对籽粒形态和充实的影响及其与粒重的关系[J]. 作物学报, 2025, 51(5): 1347-1362.
[15] 盛倩男, 方娅婷, 赵剑, 杜思垚, 胡行珍, 余秋华, 朱俊, 任涛, 鲁剑巍. 不同养分管理措施对稻田和旱地油菜产量的影响及其对冻害的响应[J]. 作物学报, 2025, 51(5): 1286-1298.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2