基于转录组分析鉴定甘蓝型油菜开花候选基因以及BnaCOR27功能验证

doi:10.3724/SP.J.1006.2025.55008

摘要/Abstract

摘要：

开花是高等植物从营养生长到生殖生长的一个重要阶段，影响生物量和种子产量。为了揭示甘蓝型油菜春化的调控网络以及挖掘重要候选基因，本研究对7个甘蓝型油菜品种春化前后的叶片转录组数据进行分析，鉴定出1305个差异表达基因，其中上调基因有554个，下调基因有751个。GO富集分析发现,差异基因主要富集在春化途径、光周期途径、昼夜节律、花发育以及冷响应等生物学过程中，进一步分析发现96个开花基因存在差异表达，包括BnaVIN3、BnaFLC、BnaCOR27等基因。此外，全基因组关联分析鉴定BnaCOR27-C04与开花时间显著关联，结合加权基因共表达网络分析，发现BnaCOR27与BnaFLC、BnaFT、BnaVIN3等基因直接相连形成潜在的网络调控甘蓝型油菜开花时间。进一步利用CRISPR/CAS9基因编辑技术对BnaCOR27进行敲除，通过对T₃代转基因植株分析，发现敲除突变体株系表现出早花表型。这一结果能对已有的甘蓝型油菜开花研究进行补充和丰富，为进一步改良开花性状提供遗传基础。

关键词: 甘蓝型油菜, 开花, 转录组分析, GWAS, BnaCOR27

Abstract:

Flowering is a critical developmental transition in higher plants, marking the shift from vegetative to reproductive growth, and it directly influences biomass accumulation and seed yield. To elucidate the regulatory network underlying vernalization in Brassica napus and identify key candidate genes, we analyzed the transcriptomes of leaves from seven rapeseed varieties before and after vernalization. A total of 1305 differentially expressed genes (DEGs) were identified, including 554 upregulated and 751 downregulated genes. GO enrichment analysis showed that these DEGs were primarily involved in biological processes such as the vernalization pathway, photoperiod pathway, circadian rhythm, flower development, and response to cold. Further analysis revealed differential expression in 96 flowering-related genes, including BnaVIN3, BnaFLC, BnaCOR27, among others. Additionally, genome-wide association studies (GWAS) indicated that BnaCOR27-C04 is significantly associated with flowering time. Integration with weighted gene co-expression network analysis (WGCNA) suggested that BnaCOR27 may interact with genes such as BnaFLC, BnaFT, and BnaVIN3, forming a potential regulatory network controlling flowering time in rapeseed. Furthermore, CRISPR/Cas9-mediated knockout of BnaCOR27 resulted in an early-flowering phenotype in T₃ transgenic plants. These findings enhance our understanding of flowering regulation in B. napus and provide a genetic foundation for improving flowering-related traits in future breeding efforts.

Key words: Brassica napus, flowering, transcriptome analysis, GWAS, BnaCOR27

王晨, 贺丹, 姚敏, 邱萍, 何昕, 熊兴华, 康雷, 刘忠松, 钱论文. 基于转录组分析鉴定甘蓝型油菜开花候选基因以及BnaCOR27功能验证[J]. 作物学报, 0, (): 0-.

WANG Chen, HE Dan, YAO Min, QIU Ping, HE Xin, XIONG Xing-Hua, KANG Lei, LIU Zhong-Song, QIAN Lun-Wen. Transcriptome-based identification of flowering candidate genes and functional characterization of BnaCOR27 in Brassicaapus[J]. Acta Agronomica Sinica, 0, (): 0-.

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