欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2008, Vol. 34 ›› Issue (05): 777-782.doi: 10.3724/SP.J.1006.2008.00777

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

糜子SAMS基因的克隆及其在干旱复水中的表达模式分析

林凡云1;王士强1;胡银岗1,2,*;何蓓如1   

  1. 1 西北农林科技大学农学院, 陕西杨凌712100; 2 陕西省农业分子生物学重点实验室, 陕西杨凌712100
  • 收稿日期:2007-09-17 修回日期:1900-01-01 出版日期:2008-05-12 网络出版日期:2008-05-12
  • 通讯作者: 胡银岗

Cloning of A S-Adenosylmethionine Synthetase Gene from Broomcorn Millet (Panicum miliaceum L.) and Its Expression during Drought and Re-Watering

LIN Fan-Yun1,WANG Shi-Qiang1,HU Yin-Gang12*,HE Bei-Ru1   

  1. 1 College of Agriculture, Northwest A & F University, Yangling 712100, Shaanxi; 2 Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, Shaanxi, China
  • Received:2007-09-17 Revised:1900-01-01 Published:2008-05-12 Published online:2008-05-12
  • Contact: HU Yin-Gang

PDF

2243

被引次数

31

摘要： 以糜子抗旱节水研究中获得的一个与S-腺苷甲硫氨酸合成酶(SAMS)基因同源性较高的EST序列为基础, 采用RT-PCR技术从糜子中分离到一个SAMS基因的全长cDNA序列(命名为PmSAMS), 全长1 293 bp, 编码396个氨基酸, 具有SAMS典型的N端结构域、中间结构域及C端结构域。糜子、马铃薯、拟南芥、甜菜、大麦、荔枝、番茄和水稻8种植物SAMS的氨基酸序列多重比较分析表明, 不同植物的SAMS的氨基酸相似程度非常高(92%~97%), 其中糜子与水稻的相似性最高(97%), 说明SAMS基因在植物进化中非常保守。PmSAMS基因在糜子幼苗干旱及复水过程中的半定量RT-PCR表达模式分析表明, 在干旱早期(土壤含水量36%)诱导该基因大量表达, 而干旱程度更严重时(土壤含水量为24%)其表达受到严重抑制, 表达量比对照还低; 严重干旱后复水2 h, 其表达量增强至干旱早期的表达量, 而复水6 h后表达量降低至对照(干旱处理前)水平。可见, PmSAMS基因的表达涉及糜子响应干旱胁迫及干旱后复水过程, 可能是糜子抗旱节水的关键基因。该基因的克隆为进一步探讨其应用于农作物抗旱节水性的遗传改良奠定了基础。

关键词: 糜子, S-腺苷甲硫氨酸合成酶, 干旱复水, 表达模式, 进化分析

Abstract: To explore more genes for the improvement of drought-tolerance and water use efficiency in plants, broomcorn millet (Panicum miliaceum L.) was used to investigate the differential gene expression during drought stress and re-watering after serious drought stress by constructing SSH-cDNA library in previous study. A full-length cDNA of S-adenosylmethionine synthetase (SAMS) gene was amplified from broomcorn millet using PCR. The PCR primers were designed based on an EST sequence highly similar to SAMS gene in the SSH-cDNA library and the sequences of SAMS genes from rice and barley. The full-length cDNA sequence of SAMS gene amplified from broomcorn millet was 1 293 bp in length and designated as PmSAMS, encoding 396 amino acids with the 3 typical domains of SAMS (N terminal domain, inter domain and C terminal domain). Multiple alignment analysis based on the amino acids encoded by some SAMS genes from broomcorn millet (P. miliaceum L.), potato (S. tuberosum), Arabidopsis (A. thaliana), sugar beet (B. vulgaris), barley (H. vulgare), litchi (L. chinensis), tomato (L. esculentum), and rice (O. sativa) indicated that SAMS was very conserved among different species of plants with 92–97% of sequence similarity of amino acids, and PmSAMS had the highest similarity with that of rice (97%). The expression patterns of PmSAMS during drought and re-watering after serious drought were investigated by means of semi-quantitative RT-PCR. The results showed that the ex-pressions of PmSAMS were great at the beginning of drought treatment (soil relative moisture of 36%), then declined under seri-ous drought (soil relative moisture of 24%) to less than the normal level, and increased at 2 h after re-watering, declined to the normal level at 6 h after re-watering. The expression patterns of PmSAMS during drought and re-watering after serious drought indicated that it was involved in the response of broomcorn millet to drought and re-watering and might be one of the key genes for drought tolerance and water use efficiency of broomcorn millet. The cloning of this gene provides the potential for its utiliza-tion in the improvement of drought-tolerance and water use efficiency in other plants.

Key words: Broomcorn millet, S-adenosylmethionine synthetase, Drought and re-watering, Express profile, Phylogenetic analysis

[1] 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[2] 张颖星, 宋裕祯, 王跃, 曹越, 曹晓宁, 王瑞云. EMS诱导糜子优异性状突变体的筛选及表型分析[J]. 作物学报, 2026, 52(5): 1388-1400.
[3] 宋裕祯, Bheel Chander Kumar, 王跃, 张颖星, 郭娟, Khound Rituraj, Santra Dipak Kumar, 曹晓宁, 王瑞云. 糜子AP2亚家族全基因组鉴定及PmAP2-1和PmAP2-9耐盐功能分析[J]. 作物学报, 2026, 52(4): 1127-1139.
[4] 胡城祯, 高维东, 孔斌雪, 王建飞, 车卓, 杨德龙, 陈涛. 小麦TaAPC11基因家族鉴定及TaAPC11-5B参与干旱胁迫的生物学功能研究[J]. 作物学报, 2026, 52(1): 148-164.
[5] 景秀清, 蔡永朵, 邓宁, 赵晓东, 翟飞红, 曾群. 藜麦RopGEF家族基因的鉴定及表达模式分析[J]. 作物学报, 2026, 52(1): 28-43.
[6] 闫知兰, 赵芹, 常甜达, 王一鸣, 王碧辉, 王鹏, 黄春国, 张会, 王利祥, 郝晓鹏, 赵波. 豆科作物AOX基因鉴定及其在普通菜豆响应非生物胁迫中的表达模式研究[J]. 作物学报, 2025, 51(7): 1769-1783.
[7] 沈傲, 刘敏, 倪迪安, 刘炜. 谷子m6A甲基转移酶基因SiMTA1的启动子序列特征和基因表达模式分析[J]. 作物学报, 2025, 51(7): 1969-1978.
[8] 周恩强, 缪亚梅, 周瑶, 姚梦楠, 赵娜, 王永强, 朱宇翔, 薛冬, 李宗迪, 石宇欣, 李波, 汪凯华, 顾春燕, 王学军, 魏利斌. 基于种子发育转录组的豌豆bZIP基因家族分析及种子发育候选基因的鉴定[J]. 作物学报, 2025, 51(4): 914-931.
[9] 徐林珊, 郜耿东, 王宇, 王家星, 杨吉招, 武亚瑞, 张宵寒, 常影, 李真, 谢雄泽, 龚德平, 王晶, 葛贤宏. 甘蓝型油菜漆酶基因家族成员表达模式及与茎秆抗折力的关联分析[J]. 作物学报, 2025, 51(1): 134-148.
[10] 丁艺冰, 辛旭霞, 冯智尊, 郭娟, 曹越, 陈喜明, 王晓丹, 曹晓宁, SANTRA Dipak K, 陈凌, 乔治军, 王瑞云. 东北春播区糜子核心种质的荧光微卫星标记鉴定[J]. 作物学报, 2024, 50(7): 1728-1739.
[11] 丁艺冰, 辛旭霞, 冯智尊, 曹越, 郭娟, Dipak K SANTRA, 王瑞云, 陈喜明. 东北春播区糜子核心种质及其DNA分子身份证构建[J]. 作物学报, 2024, 50(5): 1181-1192.
[12] 王添宁, 冯雅岚, 琚吉浩, 吴毅, 张均, 马超. 小麦及其祖先物种GRF转录因子家族鉴定与表达分析[J]. 作物学报, 2024, 50(4): 897-813.
[13] 曹越, 张立媛, 辛旭霞, 冯智尊, 郭娟, 王晓丹, 曹晓宁, SANTRA Dipak K, 陈凌, 乔治军, 王瑞云. 基于荧光SSR的宁夏糜子DNA分子身份证的构建[J]. 作物学报, 2024, 50(11): 2699-2711.
[14] 郭娟, 辛旭霞, 冯智尊, 曹越, 王晓丹, 曹晓宁, 陈凌, 乔治军, 王瑞云. 基于SSR的陕西糜子种质资源的分子鉴定[J]. 作物学报, 2024, 50(10): 2643-2653.
[15] 左春阳, 李亚玮, 李焱龙, 金双侠, 朱龙付, 张献龙, 闵玲. 陆地棉漆酶基因家族成员表达模式分析[J]. 作物学报, 2023, 49(9): 2344-2361.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2