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作物学报 ›› 2008, Vol. 34 ›› Issue (12): 2160-2167.doi: 10.3724/SP.J.1006.2008.02160

• 耕作栽培·生理生化 • 上一篇    下一篇

不同土壤条件下山农12小麦籽粒HMW-GS积累及GMP粒度分布特征

梁太波1,2;尹燕枰1;蔡瑞国1;闫素辉1;李文阳1;耿庆辉1;王平1;邬云海1;李勇1;王振林1,*   

  1. 1 山东农业大学农学院 / 作物生物学国家重点实验室, 山东泰安271018；2 中国烟草总公司郑州烟草研究院，河南郑州450001
  • 收稿日期:2008-04-11 修回日期:2008-07-14 出版日期:2008-12-12 网络出版日期:2008-10-10
  • 通讯作者: 王振林
  • 作者简介:梁太波(1981-)男,山东潍坊人,博士,从事作物高产优质生理生化与栽培学研究
  • 基金资助:

    国家自然科学基金项目(30571099);教育部高等学校博士学科点专项科研基金项目(20060434006);山东省自然科学基金会项目(Y2005D13)

HMW-GS Accumulation and GMP Size Distribution in Grains of SN12 Grown in Different Soil Conditions

LIANG Tai-Bo12,YIN Yan-Ping1,CAI Rui-Guo1,YAN Su-Hui1,LI Wen-Yang1,GENG Qing-Hui1,WANG Ping1,WU Yun-Hai1,LI Yong1,WANG Zhen-Lin1*   

  1. 1 Agronomy College, Shandong Agricultural University / National Key Laboratory of Crop Biology, Tai’an 271018, Shandong; 2 Zhengzhou Tobacco Research Institute of China National Tobacco Corporation, Zhengzhou 450001, Henan, China
  • Received:2008-04-11 Revised:2008-07-14 Published:2008-12-12 Published online:2008-10-10
  • Contact: WANG Zhen-Lin

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1858

被引次数

44

摘要：

在沙壤、中壤和黏壤3种质地土壤条件下，以优质强筋小麦品种山农12为材料研究了小麦强势与弱势籽粒高分子量谷蛋白亚基(HMW-GS)积累特征及其与谷蛋白大聚合体(GMP)粒度分布的关系。结果表明，3种质地土壤上，小麦强、弱势籽粒HMW-GS花后14 d均已形成，强势粒HMW-GS含量明显高于弱势粒，说明强势粒具有较强的HMW-GS积累能力。小麦籽粒HMW-GS含量和GMP含量均表现为黏壤土>中壤土>沙壤土，说明黏壤土有利于HMW-GS的积累。小麦GMP粒径分布范围在0.37~245 μm之间；数目分布呈单峰曲线，体积和表面积分布呈双峰曲线。小麦强势粒GMP>100 μm颗粒数目百分比和体积百分比均显著高于弱势粒，强势粒具有更多的大粒径GMP颗粒。小麦HMW-GS含量和GMP含量与<10 μm和<100 μm GMP 颗粒体积百分比均呈显著或极显著负相关，与>100 μm GMP颗粒体积百分比呈显著或极显著正相关，说明大粒径GMP颗粒具有较高的HMW-GS含量。

关键词: 小麦, 高分子量谷蛋白亚基(HMW-GS), 谷蛋白大聚合体(GMP), 土壤质地, 强势粒, 弱势粒

Abstract:

Glutenin macropolymer (GMP), the important component of wheat (Triticum aestivum L.) glutenin polymer, consists of high molecular weight gultenin subunits (HMW-GS) and low molecular weight gultenin subunits (LMW-GS). Accumulations of HMW-GS and GMP play key roles in grain quality of wheat. According to recent studies, GMP is a particle network in endosperm of wheat. The content of GMP is affected by HMW-GS accumulation and environment factors. But to date, there are few reports about the relationship between HMW-GS accumulation and GMP particle distribution. To study the HMW-GS accumulation and its relation to GMP particle distribution, a pool experiment was carried out with wheat cultivar Shannong 12 grown under three soil textures. Forty spikes was sampled at 7, 14, 21, 28, and 35 d after anthesis, and partitioned into two groups, superior grain (the 2nd grain from the basal part of a spikelet) and inferior grain (the 4th grain from the basal part of a spikelet). HMW-GS was separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The result showed that all HMW-GS already formed at 14 d after anthesis both in superior grain and inferior grain. Higher HMW-GS content showed that superior grain had strong HMW-GS accumulating ability than inferior grain. HMW-GS accumulation and GMP content of wheat grown in clay loam soil were higher than those in sandy and loam soil, which indicated that clay loam soil was suitable for accumulating of HMW-GS. The diameter of GMP particle was in 0.37–245 μm, it changed in the pattern of two-peak curve in volume and surface area distribution, while single-peak curve in number distribution of GMP particle. The percentages of number and volume of >100 μm GMP particle were higher in superior grain than in inferior grain. The contents of HMW-GS and GMP were negatively correlated with GMP particle volume of <10 μm and <100 μm, but positively correlated with that of >100 μm. It is suggested that larger GMP particle has more HMW-GS content.

Key words: Wheat (Triticum aestivum L.), High molecular weight glutenin subunits (HMW-GS), Glutenin macropolymer (GMP), Size distribution, Soil texture, Superior grain, Inferior grain

[1]Sun H(孙辉), Yao D-N(姚大年), Liu G-T(刘广田), Zhang S-Z(张树榛). Study on relationships of wheat protein to bak-ing quality: I. Relationships of protein and protein fraction contents to baking quality. J Chin Cereals Oils Assoc (中国粮油学报), 2001, 16(2): 28-30 (in Chinese with English ab-stract)
[2]Sapirstein H D, Fu B X. Intercultivar variation in the quantity of monomeric proteins, soluble and insoluble glutenin, and residue protein in wheat flour and relationships to bread making quality. Cereal Chem, 1998, 75: 500-507
[3]Sun H(孙辉), Yao D-N(姚大年), Li B-Y(李宝云), Liu G-T(刘广田), Zhang S-Z(张树榛). Correlation between content of glutenin macropolymer (GMP) in wheat and baking quality. J Chin Cereals Oils Assoc (中国粮油学报), 1998, 13(6): 13-16(in Chinese with English abstract)
[4]Zhao H-X(赵惠贤), Hu S-W(胡胜武), Ji W-Q(吉万全), Xue X-Z(薛秀庄), Guo A-G(郭蔼光), Mares D. Study on rela-tionship between the size distribution of glutenin polymeric protein and wheat flour mixing properties. Sci Agric Sin (中国农业科学), 2001, 34(5): 465-468(in Chinese with English abstract)
[5]Don C, Lichtendonk W, Plijter J J, Hamer R J. Glutenin macropolymer: A gel formed by glutenin particles. J Cereal Sci, 2003, 37: 1-7
[6]Don C, Lookhart G, Naeem H, MacRitchie F, Hamer R J. Heat stress and genotype affect the glutenin particles of the glu-tenin macropolymer-gel fraction. J Cereal Sci, 2005, 42: 69-80
[7]Deng Z-Y(邓志英), Tian J-C(田纪春), Zhang Y-X(张永祥), Wang Y-X(王延训). Formation time and accumulation inten-sity of HMW-GS and LMW-GS and the relationship with SDS-sedimentation volume in winter wheat. Acta Agron Sin (作物学报), 2005, 31(3): 308-315(in Chinese with English abstract)
[8]Gianibelli M C, Gupta R B, Lafiandra D, Margiotta B, Macritchie F. Polymorphism of high Mr glutenin subunits in Triticum tauschii: Characterization by chromatography and electrophoretic methods. J Cereal Sci, 2001, 33: 39-52
[9]Weegels P L, van de Pijpekamp A M, Graveland A, Hamer R J, Schofield J D. Depolymerisation and re-polymerisation of wheat glutenin during dough processing: I. Relationships be-tween glutenin macropolymer content and quality parameters. J Cereal Sci, 1996, 23: 103-111
[10]Don C, Mann G, Bekes F, Hamer R J. HMW-GS affects the properties of glutenin particles in GMP and thus flour quality. J Cereal Sci, 2006, 44: 127-130
[11]Yue H-W(岳鸿伟), Qin X-D(秦晓东), Dai T-B(戴廷波), Jing Q(荆奇), Cao W-X(曹卫星), Jiang D(姜东). Effects of nitro-gen rate on accumulations of HMW-GS and GMP in wheat grain. Acta Agron Sin (作物学报), 2006, 32(10): 1678-1683 (in Chinese with English abstract)
[12]Sun X(孙霞), Wu X-J(吴秀菊), Hu S-L(胡尚连), Li W-X(李文雄). Effects of different nitrogen sources on HMW glutenin aggregates in spring wheat with different HMW-GS composi-tion. Acta Agron Sin (作物学报), 2005, 31(3): 308-315(in Chinese with English abstract)
[13]Zhao J-Y(赵俊晔), Yu Z-W(于振文). Effects of nitrogen fer-tilizer rate on nitrogen metabolism and protein synthesis of superior and inferior wheat kernel. Sci Agric Sin (中国农业科学), 2005, 31(4): 463-468(in Chinese with English abstract)
[14]Wang R-Y(王瑞英), Yu Z-W(于振文), Pan Q-M(潘庆民), Xu Y-M(许玉敏). Changes of endogenous plant hormone con-tents during grain development in wheat. Acta Agron Sin (作物学报), 1999, 25(2): 227-231(in Chinese with English ab-stract)
[15]Liang R-Q(梁荣奇), Zhang Y-R(张义荣), You M-S(尤明山), Mao S-F(毛善锋), Song J-M(宋建民), Liu G-T(刘广田). Multistacking SDS-PAGE for wheat glutenin polymer and its relation to bread making quality. Acta Agron Sin (作物学报), 2002, 28(5): 609-614(in Chinese with English abstract)
[16]Don C, Lichtendonk W J, Plijter J J, Vliet T, Hamer R J. The effect of mixing on glutenin particle properties: aggregation factors that affect gluten function in dough. J Cereal Sci, 2005, 41: 69-83
[17]Ru Z-G(茹振钢), Li G(李淦), Hu T-Z(胡铁柱), Li L-B(栗利波). Analysis of grain weight and quality at different floret position of strong glutinin wheat. J Triticeae Crops (麦类作物学报), 2006, 26(5): 134-136(in Chinese with English ab-stract)
[18]Li J-C(李金才), Wei F-Z(魏凤珍), Ding X-P(丁显萍). Rela-tionship between vascular bundle system of rachis and ra-chilla and ear productivity in wheat. Acta Agron Sin (作物学报), 1999, 25(3): 315-319(in Chinese with English abstract)
[19]Li C-H(李潮海), Li S-L(李胜利), Wang Q(王群), Hou S(侯松), Jing J(荆棘). Effect of different textural soils on root dynamic growth in corn. Sci Agric Sin (中国农业科学), 2004, 37(9): 1334-1340(in Chinese with English abstract)
[20]Masoni A, Ercoli L, Mariotti M, Arduini I. Post-anthesis accumulation and remobilization of dry matter, nitrogen and phosphorus in durum wheat as affected by soil type. Eur J Agron, 2007, 26: 179-186
[21]Wang F-C(王凤成), Zhu J-B(朱金宝), Khan K, Brien L O, Chen W-Y(陈万义). The composition and quantity of wheat glutenin subunits in relation to high molecular weight glu-tenin polymers and their effects on bread making quality. J Chin Cereals Oils Assoc (中国粮油学报), 2004, 19(3): 13-17(in Chinese with English abstract)
[22]Gupta R B, Khan K, MacRitchie F. Biochemical basis of flour properties in bread wheats: I. Effects of variation in the quan-tity and size distribution of polymeric protein. J Cereal Sci, 1993, 18: 23-41
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