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作物学报 ›› 2009, Vol. 35 ›› Issue (4): 679-684.doi: 10.3724/SP.J.1006.2009.00679

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

中国与东南亚三国（越、老、柬）普通野生稻遗传多样性的比较研究

孙希平12，杨庆文2*   

  1. 1山西农业大学生物工程中心，山西太谷030801；2中国农业科学院作物科学研究所，北京100081
  • 收稿日期:2008-08-17 修回日期:2008-10-18 出版日期:2009-04-12 网络出版日期:2009-02-16
  • 通讯作者: 杨庆文
  • 基金资助:

    本研究由热带亚热带优势农作物品种繁育技术研究与示范项目（2007BAD68B01）资助。

Comparative Study on Genetic Diversity of Wild Rice(Oryza rufipogon Griff.)in China and Three Countries in Southeast Asia

SUN Xi-Ping12,YANG Qing-Wen2*   

  1. 1Shanxi Agricultural University,Taigu 030801,China;2Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2008-08-17 Revised:2008-10-18 Published:2009-04-12 Published online:2009-02-16
  • Contact: YANG Qing-Wen

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被引次数

12

摘要：

利用24对SSR引物，对来自中国10个代表性居群和越南、老挝、柬埔寨的5个居群共计282份普通野生稻材料进行遗传多样性比较研究。结果表明，(1)24个微卫星位点共检测到等位基因289个，平均等位基因数(A)为12，有效等位基因数(Ae)为7；期望杂合度(He)平均为0.812，观察杂合度(Ho)平均为0.543，香农指数(I)平均为1.99；(2)4个国家中，中国普通野生稻的遗传多样性最丰富，老挝次之，越南和柬埔寨最低，香农指数(I)分别为1.92、1.69、1.47和1.45；(3)采用UPGMA方法对供试15个居群进行聚类分析。老挝和柬埔寨2个居群的亲缘关系最近，二者与越南居群有着较近的亲缘关系；(4)在中国所有参试居群中，广西武宣和贺州居群与东南亚3国亲缘关系最近。

关键词: 普通野生稻, SSR, 遗传多样性

Abstract:

Simple sequence repeat (SSR) analysis was performed to assess the genetic diversity of Oryza rufipogon Griff. using 10 representative populations from China, 2 populations from Vietnam, 1 population from Laos and 2 populations from Cambodia. A total of 282 individuals were screened using 24 different SSR primers. Relationship among populations was analyzed based on the UPGMA cluster method. The results were as follows: (i) Two hundred and eighty nine alleles were detected at 24 microsatellites loci; the average number of alleles (A) was 12; the average effective number of alleles (Ae) was 7; the expected heterozygosity (He) was 0.812; the observed heterozygosity (Ho) was 0.543; the Shannon’ index (I) was 1.99, which meant that the detected populations from 4 countries were rich of genetic diversity. (ii) The populations from China had the most abundant genetic diversity with I of 1.92, while those from Laos, Vietnam and Cambodia were with I of 1.69, 1.47, and 1.45 respectively. (iii) Populations from Laos and Cambodia had the closest relationship, while populations from Vietnam, Laos and Cambodia had the closer relationships to each other than to those from China. (iv) Compared with the other populations in China, these from Wuxuan and Hezhou had the closer relationships to these from Southeast countries.

Key words: Oryza rufipogon Griff., Simple sequent repeat(SSR), Genetic diversity

[1]Barbier P. Genetic variation and ecotypic differentiation in the wild rice species Oryza fufipogon: I. Population differentiation in life-history traits and isozymic loci. Jpn J Genet, 1989, 64: 259–271
[2]Barbier P. Genetic variation and ecotypic differentiation in the wild rice species Oryza rufipogon: II. Influence of the mating system and life-history traits on the genetic structure of populations. Jpn J Genet, 1989, 64: 273–285
[3]Morishima H, Barbier P. Mating system and genetic structure of natural populations in wild rice Oryza rufipogon. Plant Species Biol, 1990, 5: 31–39
[4]Gao L-Z(高立志). Genetic Conservation of Three Wild Rice in China (中国三种野生稻的遗传基因与保护生物学研究). PhD Dissertation of Institute of Botany, Chinese Academy of Sciences, 1997 (in Chinese with English abstract)
[5]Gao L-Z(高立志), Zhou Y(周毅), Ge S(葛颂), Hong D-Y(洪德元), Liang Y-M(梁耀懋), Lin D-H(林登豪), Chen C-B(陈成斌), Wu M-L(吴妙燊), Dong D-A(董德爱). Current status of the genetic resources of Oryza rufipogon Griff. and its conservation strategies in Guangxi. Sci Agric Sin (中国农业科学), 1998, 31(1): 32–39 (in Chinese with English abstract)
[6]Gao L Z, Ge S, Hong D Y. Low levels of genetic diversity within populations and high differentiation among populations of a wild rice Oryza granulata from China. Int J Plant Sci, 2000, 161: 691–697
[7]Zhu Z-F(朱作峰), Sun C-Q(孙传情), Fu Y-C(付永彩), Zhang P-J(张培江), Wang X-K(王象坤). Genetic diversity of Oryza sativa and Oryza rufipogon by SSR. Sci Agric Sin(中国农业科学), 2002, 35(12):1437–1441 (in Chinese with English abstract)
[8]Wang Y-H(王艳红), Wang H(王辉), Gao L-Z(高立志). Genetic diversity of Oryza rufipogon by SSR. Acta Bot Boreali-Occident Sin (西北植物学报), 2003, 23(10): 1750–1754(in Chinese with English abstract)
[9]Xie J-K(谢建坤), Chen Q-L(陈庆隆), Xiao Y-Q(肖叶青), Chen D-Z(陈大洲), Qiu B-Y(邱兵余), Wan Y(万勇). SSLP analysis of nuclear genome in Dongxiang wild rice. Acta Agric Jiangxi (江西农业学报), 2002, 14(3): 1–6(in Chinese with English abstract)
[10]Yang Q-W(杨庆文), Yu L-Q(余丽琴), Zhang W-X(张万霞). Comparative studies on genetic diversities between in-situ and ex-situ conserved germplasm of Oryza rufipogon. Sci Agric Sin(中国农业科学), 2005, 38(6):1073–1079(in Chinese with English abstract)
[11]Ren M(任民), Chen C-B(陈成斌), Rong T-Z(荣廷昭), Zhang W-X(张万霞), Ge H-M(盖红梅), Yang Q-W(杨庆文). Genetic diversity of Oryza rufipogon Griff. in southeast region of Guangxi in China. Plant Genet Resour (植物遗传资源学报), 2005, 6(6): 1–8 (in Chinese with English abstract)
[12]Ge H-M(盖红梅), Chen C-B(陈成斌), Shen F-F(沈法富), Zhang W-X(张万霞), Ren M(任民), Wang Y-W(王玉微), Yang Q-W(杨庆文). Genetic diversity and conservation strategy of Oryza rufipogon along the Haojiang River in Guangxi Zhuang Autonomous Region. Plant Genet Resour (植物遗传资源学报), 2005, 6(2): 156–162 (in Chinese with English abstract)
[13]Condit R, Hubbell S P. Abundance and DNA sequence of two base repeat regions in tropical tree genomes. Genome, 1991, 34: 66–71
[14]Bowcock A, Tomfohrde J, Minch E, Kidd J, Cavallisforza L. High resolution of human evolutionary trees with polymorphic microsatellites. Nature, 1994, 368: 455–457
[15]Bruford J, Wayne R. Microsatellites and their application to population genetic studies. Curr Opin Genet Dev, 1993, 3: 939–943
[16]Hall P, Chase M, Bawa K. Low genetic variation but high population differentiation in a common tropical forest tree species. Conserv Biol, 1994, 8: 471–482
[17]Terauchi R, Konuma A. Microsatellite polymorphism in Dioscorea tokoro, a wild yam species. Genome, 1994, 37: 794–801
[18]Chase M, Kesseli R, Bawa K. Microsatellite markers for population and conservation genetics of tropical trees. Am J Bot, 1996, 83: 51–57
[19]Provan J, Russell R, Booth A, Powell W. Polymorphic chloroplast simple sequence repeat primers for systematic and population studies in the genus Hordeum. Mol Ecol, 1999, 8: 505–512
[20]Han D-F(韩东飞). Genetic Diversity and Conservation of in China. MS Dissertation of Graduate School Chinese Academy of Agricultural Science, 2006(in Chinese with English abstract)
[21]Sun X-P(孙希平), Yang Q-W(杨庆文), Li R-Z(李润植). Genetic diversity of three wild rice species in Hainan province. Acta Agron Sin (作物学报), 2007, 33(7): 1100–1107(in Chinese with English abstract)
[22]Nei M. Estimation of average heterozygosity genetic distance from a small number of individuals. Genetics, 1978, 89: 583–590
[23]Wright S. Surface of selective values revisited. Am Nat, 1978, 131: 115–123
[24]Kuroda Y, Yoichiro S, Chay B, Yasuyuki K, Koji T. Genetic structure of three Oryza AA genome species (O. rufipogon, O. nivara and O. sativa) as assessed by SSR analysis on the Vientiane plain of Laos. Conserv Genet, 2007, 8: 149–158
[25]Sun C-Q(孙传清), Wang X-K(王象坤), Ji C-C(吉村淳). A study of the genetic diversity of common wild rice (Oryza rufipogon Griff.) and cultivated Rice (Oryza sativa L.) by RFLP Analysis. Acta Genet Sin (遗传学报), 2000, 27(3): 227–234 (in Chinese with English abstract)
[26]Yang Q-W(杨庆文). Genetic Diversity and Conservation of Oryza rufipogon Griff. in China. PhD Dissertation of Chinese Agricultural University, 2004(in Chinese with English abstract)
[27]Yang Q-W(杨庆文), Dai L-Y(戴陆园), Shi J-X(时津霞), Zhang W-X(张万霞), Ren J-F(任军方), Miao H(苗晗). Study of genetic diversity and conservation strategy of (Oryza rufipogon Griff.) in Yuanjiang. Plant Genet Resour (植物遗传资源学报), 2004, 5(1): 1–5 (in Chinese with English abstract)
[28]Yan H-J(晏慧君), Fu J(付坚), Li J(李俊), Jia X-Q(贾兴奇), Wang L-X(王玲仙), Cheng Z-Q(程在全). Genetic diversity and relationship of (Oryza rufipogon Griff.) in Yunnan. Acta Bot Sin (植物学通报), 2006, 23(6): 670–676 (in Chinese with English abstract)
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