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作物学报 ›› 2009, Vol. 35 ›› Issue (6): 992-997.doi: 10.3724/SP.J.1006.2009.00992

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

马铃薯晚疫病抗性基因R11的遗传定位

徐建飞，金黎平，段绍光，黄三文，屈冬玉*   

  1. 中国农业科学院蔬菜花卉研究所，北京100081
  • 收稿日期:2008-11-24 修回日期:2009-02-17 出版日期:2009-06-12 网络出版日期:2009-04-16
  • 通讯作者: 屈冬玉，E-mail: dyqu@mail.caas.net.cn;Tel:010-82109543
  • 作者简介:徐建飞，E-mail:xujianfei1026@126.com
  • 基金资助:

    本研究由国家自然科学基金项目（30671319），国家科技支撑计划（2007BAD49A01），农业部园艺作物遗传改良重点开放实验室资助。

Genetic Mapping of Rll Gene Conferring Resistance to Late Blight in Potato(Solanum tuberosum)

XU Jian-Fei,JIN Li-Ping,DUAN Shao-Guang,HUANG San-Wen,QU Dong-Yu*   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Received:2008-11-24 Revised:2009-02-17 Published:2009-06-12 Published online:2009-04-16
  • Contact: QU Dong-Yu，E-mail: dyqu@mail.caas.net.cn;Tel:010-82109543
  • About author:XU Jian-Fei，E-mail:xujianfei1026@126.com

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被引次数

11

摘要：

由致病疫霉菌(Phytophthora infestans)引起的晩疫病是世界范围内最具毁灭性的马铃薯(Solanum tuberosum)病害。以含有晚疫病抗性基因R11的材料MaR11和不含已知抗性基因的品种Katahdin为亲本进行有性杂交，对获得的F1分离群体的83个基因型进行了晚疫病菌株接种鉴定和遗传分析。结果表明，R11为主效单基因，在MaR11中以单式形式(R11r11r11r11)存在。应用比较作图和分离群体分组分析(BSA)，开发了6个与R11连锁的分子标记，并将R11定位于11号染色体长臂末端。R11距C2_At5g59960标记最近，约为2.4 cM。通过遗传图谱比较表明，R11较晚疫病抗性基因R3a和R10更靠近染色体端粒区。本研究所获得的遗传图谱为进一步构建R11高密度遗传图谱提供了基础。

关键词: 马铃薯, 晚疫病, Rll , 遗传定位

Abstract:

Late blight caused by Phytophthora infestans is the most destructive disease for potato (Solanum tuberosum). A F1 segregating population consisting of 83 genotypes, derived from R11 differential MaR11 and the susceptible potato cultivar Katahdin, was assessed for late blight resistance in detached leaves assay and genetically analyzed. The results showed that the distribution for R11 was clearly bimodal with the two peaks coinciding with the blight scores of the MaR11 (the resistant parent) and Katahdin (the susceptible parent), and the observed segregation with 39 resistant and 44 susceptible plants fitted a 1:1 ratio. It indicated that R11 was inherited as a major dominant R gene and presented in the simplex condition in MaR11. There was a major late blight resistance locus (MLB) in potato on the long arm of chromosome 11, where R11 showed allelic versions of the R3 and R10 loci. Using the combination of comparative mapping and bulked segregant analysis, we developed six markers linked to R11 which was located on the end of the long arm of chromosome 11 with a distance of about 2.4 cM to marker C2_At5g59960. R11 was telomeric to the R3a and R10 gene. The genetic map constructed in this paper provides a basis for further construction of high-resolution genetic map of the R11 gene.

Key words: Potato, Late blight, Rll, Genetic mapping

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