陕麦139抗条锈病基因遗传分析

doi:10.3724/SP.J.1006.2010.00109

摘要/Abstract

摘要：

利用常规遗传和单缺体遗传分析方法，研究了小麦抗条锈病新种质陕麦139中抗病基因的遗传方式。结果表明，陕麦139×辉县红和陕麦139×阿勃两组合F₁植株对条中32表现近免疫。F₂群体对条中32抗性调查表明, 陕麦139×阿勃组合和陕麦139×辉县红组合的抗感比例分别为203∶16和210∶13，经卡方检验, 抗感分离比符合15∶1 (χ²值分别为0.26和0.02, χ²_0.05,1 = 3.84), 说明陕麦139所含抗性基因对条中32的抗性受2对独立遗传显性位点控制。21个单缺体组合的F₂群体苗期室内接种条中32的抗性分离调查结果表明，阿勃1BN´陕麦139组合抗感分离比例为75∶0 (χ²=4.65，χ²_0.05,1 = 3.84)，阿勃2DN´陕麦139组合抗感分离比例为132∶2 (χ²=4.40，χ²_0.05,1 = 3.84)，远远偏离15∶1，其余19个组合的抗感分离比例经卡方测验均符合15∶1。表明该抗条锈病基因位于1B和2D染色体，暂被分别命名为YrSM139-1B和YrSM139-2D。利用284对SSR引物检测F₂群体的抗感池和单株，发现YrSM139-1B与SSR标记Xgwm273紧密连锁，即该标记可作为YrSM139-1B抗条锈病基因的标记。利用Xgwm273对陕麦139的亲本分析表明, YrSM139-1B抗条锈病基因来自野生二粒小麦AS846。

关键词: 小麦, 抗病基因, 条锈病, 遗传分析

Abstract:

Stripe rust, caused by Puccinia striiformis West. f. sp. tritici, is one of the most damaging diseases of wheat (Triticum aestivum L.) worldwide. Application of resistant varieties has been considered as the most economical and environment-friendly approach to control the disease. Resistance to stripe rust is frequently overcome by the pathogendue to the emergence of new virulent races. Wheat cultivarShaanmai 139 is resistant to allcurrent Chinese epidemic races of P. striiformis f. sp. tritici, CYR29, CYR30, CYR31, CYR32, and CYR33. Based on the monosomic genetic analysis, here, we reported the characterization and molecular marker of stripe rust resistance genes to CYR32 in Shaanmai 139. We screened 442 F₂ plants derived from two crosses between Shaanmai 139 and two susceptible cultivars Abbondanza and Huixianhong, respectively. The results showed the ratios of the resistant to the susceptible of F₂population from Shaanmai 139´Abbondanza and Shaanmai 139×Huixianhong were 203:16 and 210:13 respectively, corresponding to 15:1 (χ²is 0.26 and 0.02, χ²_{0.05, 1} = 3.84). Monosomic analysis showed the ratios deviated from 15:1 in the F₂ plants of Shaanmai 139×Abbondaza 1BN and Shaanmai 139×Abbondaza 2DN, while the others ratios corresponded to 15:1. Thus, two resistance loci in Shaanmai 139 were found on chromosomes 1B and 2D, tentatively designated YrSM139-1B and YrSM139-2D, respectively, according to the analysis of 21 monosomic and nullsomic lines of the cultivar Abbondanza and SSR mappingresults. One SSR marker, Xgwm273, was closely linked toYrSM139-1B in the F₂population. SSR mapping results showed that YrSM139-1B derived from a T. dicoccoides accession,AS846.

Key words: Common wheat, Resistance gene, Puccinia striiformis tritici, Genetic analysis

张宏，任志龙，胡银岗，王长有，吉万全. 陕麦139抗条锈病基因遗传分析[J]. 作物学报, 2010, 36(1): 109-114.

ZHANG Hong,REN Zhi-Long,HU Yin-Gang,WANG Chang-You,JI Wan-Quan. Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139[J]. Acta Agron Sin, 2010, 36(1): 109-114.

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