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作物学报 ›› 2010, Vol. 36 ›› Issue (1): 115-120.doi: 10.3724/SP.J.1006.2010.00115

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

粳稻米碱消值的数量性状基因座检测

黎毛毛1,2,3，徐磊1，任军芳1，曹桂兰1，余丽琴3，贺浩华2,*，韩龙植1,*，高熙宗4   

  1. 1中国农业科学院作物科学研究所/农作物基因资源与基因改良高家重大科学工程/农业部作物种质资源与生物技术重点开放实验室，北京100081；2江西农业大学农学院，江西南昌330045；3江西省农业科学院水稻研究所，江西南昌330200；4首尔大学小农业生命科学大学农学科，韩国首尔151-921
  • 收稿日期:2009-06-30 修回日期:2009-09-02 出版日期:2010-01-12 网络出版日期:2009-11-17
  • 通讯作者: 韩龙植, E-mail: lzhan58@yahoo.com.cn; Tel & Fax: 010-62176784; 贺浩华, E-mail: hhhua64@163.com
  • 基金资助:

    本研究由国际科技支撑计划项目（2006BAD13B01），国家重点基础研究发展计划（973计划）项目（2004CB117201），国家科技基础条件平台项目（2005DKA21001-01），农业部作物种子资源保护项目【NB08-2130135-(25-31)-01】资助。

Identification of Quantitative Trait Loci for Alkali Digestion Value in Japonica Rice

LI Mao-Mao1,2,3,XU Lei1,REN Jun-Fang1,CAO Gui-Lan1,YU Li-Qin3,HE Hao-Hua2,*,HAN Long-Zhi1*,KOH Heejong4   

  1. 1Insitute of Crop Sciences，Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Resources and Biotechnology，Ministry of Agriculture，Beijing100081，china;2College of Agronmy,Jiangxi Agricultural University, Nanchang 330045，China；3Rice Research Institute ，Jiangxi Academy of Agricultural Sciences，Nanchang 330200，China；4School of Plant Science，College of Agriculture and Life Science，Seoul National University，Seoul 151-921，Korea  
  • Received:2009-06-30 Revised:2009-09-02 Published:2010-01-12 Published online:2009-11-17
  • Contact: HAN Ling-Zhi, E-mail: lzhan58@yahoo.com.cn; Tel & Fax: 010-62176784; 贺浩华, E-mail: hhhua64@163.com

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被引次数

3

摘要：

利用碱消值差异显著的大粒粳稻DL115与小粒粳稻XL005杂交获得的200个单株F2群体为作图群体, 采用复合区间作图方法, 利用SSR标记进行了粳稻碱消值数量性状基因座的检测。结果表明, 在F2群体中碱消值呈正态连续分布, 表现为由多基因控制的数量性状。共检测到与碱消值相关的QTL 3个, qADV3、qADV5和qADV11, 分别位于第3、第5和第11染色体的RM14870~RM1284、RM3838~RM3351和RM1812~RM332区间, 对表型变异的解释率分别为6.5%、10.3%和8.1%。qADV5的增效等位基因来自碱消值较小的亲本XL005, qADV3和qADV11的增效等位基因来自碱消值较大的亲本DL115; 基因作用方式表现为显性或超显性。

关键词: 粳稻, 碱消值, SSR标记, 数量选择基因座

Abstract:

Using an F2 population including 200 individuals derived from a cross combination between two japonica rice DL115 with large grain and XL005 with small grain, which were significantly different on alkali digestion value (ADV), the quantitative trait loci for ADV were identified by composite interval mapping with SSR markers.The results showed that ADV exhibited a normal continuous distribution in F2 population, indicating that ADV is a quantitative trait controlled by multiple genes. A total of three QTLs conferring ADV were detected in intervals of RM14870–RM1284, RM3838–RM3351, and RM1812–RM332 on chromosomes 3, 5, and 11, and explained 6.5%, 10.3% and 8.1% of the observed phenotypic variance, respectively. The allele of qADV5 was derived from XL005 with less ADV, and the alleles of qADV3 and qADV11 were derived from DL115 with bigger ADV. The gene action was controlled by dominance and over-dominance.

Key words: Japonica rice, Alkali digestion value（ADV）, SSR Marker, Quantitative Traits locus

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