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作物学报 ›› 2010, Vol. 36 ›› Issue (1): 177-183.doi: 10.3724/SP.J.1006.2010.00177

• 研究简报 • 上一篇    下一篇

甘蔗SSR和AFLP分子遗传连锁图谱构建

刘新龙1,2，毛钧1,2，陆鑫1,2，马丽1,2，Karen Sarah AITKEN4，Phillip Andrew JACKSON4，蔡青1，3，范源洪1，2，*   

  1. 1云南省甘蔗遗传改良重点实验室，云南开远661600；2云南省农业科学院甘蔗研究所，云南开远661600；2云南省农业科学院生物技术与种质资源研究所，云南昆明650223；4澳大利亚联邦科学与工业研究组织（CSIRO）植物研究中心，澳大利亚Brisbane，QLD4067
  • 收稿日期:2009-06-03 修回日期:2009-08-31 出版日期:2010-01-12 网络出版日期:2009-11-17
  • 通讯作者: 范源洪, E-mail: fyhysri@vip.sohu.com
  • 基金资助:

    本研究由中-澳合作项目（CS1/2000/038），云南省应用基础研究计划重点项目（2006C0013Z），国家科技基础条件平台工作项目子专题（2007DKA21002-11）资助。

Construction of Molecular Genetic Linkage Map of Sugarcane Based on SSR and AFLP Markers

LIU Xin-Long1,2,MAO Jun1,2,LU Xin1,2,MA Li1,2,Karen Sarah AITKEN 4,Phillip Andrew JACKSON4,CAI Qing1,3,FAN Yuan-Hong1,2,*   

  1. 1 Yunnan Key Laboratory of Sugarcane Genetic Improvement, Kaiyuan 661600, China; 2 Sugarcane Research Institute, Yunnan Academy of Agricultural Sciences, Kaiyuan 661600, China; 3 Biotechnology & Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China; 4 CSIRO Division of Plant Industry, Brisbane, QLD 4067, Australia
  • Received:2009-06-03 Revised:2009-08-31 Published:2010-01-12 Published online:2009-11-17
  • Contact: FAN Yuan-Hong, E-mail: fyhysri@vip.sohu.com

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1972

被引次数

42

摘要：

采用甘蔗商业品种Co419与野生种割手密Y75/1/2杂交，获得269个单株，组成F1群体，用F102/356与商业品种ROC25回交获得266个单株，组成BC1群体。利用筛选的多态性条带丰富的36对SSR引物和12对AFLP引物，对两个群体进行PCR扩增和分子遗传连锁分析，构建甘蔗分子遗传连锁图谱。用F1群体获得630个分离标记，经c2检测，298个标记为单双剂量标记，占总标记数的47%；用BC1群体获得571个分离标记，有264个标记为单双剂量标记，占总标记数的46%；4个亲本获得单双剂量标记的数量依次为Co419>02/356>Y75/1/2>ROC25。在LOD≥5.0，相邻标记遗传距离≤40 cM的条件下，F1群体有134个单双剂量标记被纳入55个连锁群，其中39个连锁群归属8个同源组，16个未列入，总遗传距离为1 458.3 cM，标记间平均图距为10.9 cM；BC1群体有133个单双剂量标记被纳入47个连锁群，其中34个连锁群归属于8个同源组，13个连锁群未列入，总遗传距离为1 059.6 cM，标记间平均图距为8.0 cM。从4个亲本单双剂量标记进入的连锁群数来看，Co419最多，归入34个连锁群，其次为Y75/1/2，归入20个连锁群，第3为02/356和ROC25，归入19个连锁群。研究结果表明, 从单双剂量标记比例、形成连锁群数量、总遗传距离来看，F1群体构图质量要优于BC1群体。

关键词: 甘蔗, 遗传连锁图谱, SSR, AFLP, 群体

Abstract:

To take advantage of the MAS technique and develop the molecular genetic linkage map in sugarcane breeding program in China, we constructed two molecular genetic maps by single dose markers (SDM) and double dose markers (DDM) from 36 SSR and 12 AFLP primers for two mapping populations (F1 and BC1). F1 population was from cross combination of sugarcane commercial variety (Co419) × S. spontaneum (Y75/1/2) consisting of 269 true individuals, BC1 population from backcross combination of F102/356×sugarcane commercial variety (ROC25) with 266 true individuals. All plants from two populations were detected by PCR.

Two molecular genetic linkage maps were established. 298 SDMs and DDMs were acquired by c2 test from 630 segregation markers of F1 population, about 47% of total markers; 264 SDMs and DDMs were obtained from 571 segregation markers of BC1 population which were 46% of total markers, Co419>02/356>Y75/1/2>ROC25 in efficiency of getting SDMs and DDMs. Based on LOD ≥ 5.0, genetic distance ≤ 40 cM between markers, 134 of 298 SDMs and DDMs from F1 population formed 55 linkage groups (LGs), among which 39 LGs were included in eight homology groups(HGs), 16 LGs were excluded, the map length reached 1 458.3 cM with an average distance of 10.9 cM between markers; 133 of 298 SDMs and DDMs from BC1 population made up 47 LGs , among which 34 LGs were composed in eight HGs except for 13 LGs, the map covered 1 059.6 cM with an average distance of 8.0 cM. At the same time, these SDMs and DDMs from Co419 and Y75/1/2 formed 34 LGs and 20 LGs, respectively, and these SDMs from two parents only made up 1 LGs; 19 LGs were obtained from these SDMs and DDMs of 02/356, and ROC25, 9 LGS were formed by SDMs from two parents; Co419>Y75/1/2>02/356=ROC2 according to forming number of LGs. These results indicated that F1 mapping population was better than BC1 mapping population in quality of constructing linkage map. The maps will provide the valuable information for locating gene, further research on genetic linkage map and MAS breeding.

Key words: Sugarcane, Genetic Linkage map, SSR, AFLP, Population

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