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作物学报 ›› 2010, Vol. 36 ›› Issue (1): 184-190.doi: 10.3724/SP.J.1006.2010.00184

• 研究简报 • 上一篇    

水稻“斑马叶”突变体b411叶绿体超微结构的观察

邱义兰1，李红1,2，彭克勤3，刘珠丽1，陈松1，刘如石1，梁满中1，陈良碧1,*   

  1. 1湖南师范大学生命科学学院，湖南长沙410081；2湖南省宁乡县第一高级中学，湖南宁乡410600；3河南农业大学生物科学与技术学院，湖南长沙410128
  • 收稿日期:2009-06-11 修回日期:2009-09-04 出版日期:2010-01-12 网络出版日期:2009-11-17
  • 通讯作者: 陈良碧, E-mail: chenliangbi@126.com; Tel: 0731-8872617
  • 基金资助:

    本研究由湖南省自然科学基金项目（20080440983），湖南省重大科技专项（2009fj1012）资助。

Observation of Chloroplast Ultrastructure of Zebra Leaf in Rice Mutant b411

QIU Yi-Lan1,LI Hong1,2,PENG Ke-Qin3,LIU Zhu-Li1,CHEN Song1,LIU Ru-Shi1,LIANG Man-Zhong1，CHEN Liang-Bi1*   

  1. 1 College of Life Sciences, Hunan Normal University, Changsha 410081, China; 2 Ningxiang First Senior Middle School, Ningxiang 410600, China; 3.College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China
  • Received:2009-06-11 Revised:2009-09-04 Published:2010-01-12 Published online:2009-11-17
  • Contact: CHEN Liang-Bi, E-mail: chenliangbi@126.com; Tel: 0731-8872617

PDF

2073

被引次数

9

摘要：

采用透射电镜技术，对水稻突变体B411“斑马叶”出现过程中叶片的叶绿体超微结构进行了研究。结果表明，在叶片包于叶鞘内的心叶期，叶片绿区叶绿体呈纺锤形，类囊体片层纵向整齐排列；黄区叶绿体呈不规则椭圆形，类囊体片层排列不规则。在叶片抽出叶鞘1 d的嫩叶期，叶片绿区叶绿体类囊体片层增多并垛叠形成基粒结构，它们沿长轴整齐排列；黄区叶绿体类囊体膜断裂，片断化。在成熟期，叶片绿区叶绿体类囊体片层变得丰富和形成许多基粒结构；叶片黄区叶绿体内部结构严重解体，整个叶绿体呈高电子密度的囊泡状结构。在复绿期，叶片黄区逐渐变成绿色，叶绿体结构恢复正常，类囊体膜系统重建，且有序地沿叶绿体长轴方向排列，在基质中形成淀粉粒，表明其光合能力恢复。

关键词: 水稻, 斑马叶, 叶绿体, 超微结构

Abstract:

A rice zebra leaf mutant B411 was examined using transmission electron microscopy technique to study the chloroplast ultrastructure during development of zebra leaf. The results showed that there was a distinct difference between green and yellow zones of the same leaf when the leaf was enwrapped in the sheath. Chloroplasts were spindle-shaped with thylakoid membranes neatly arranged along the longitudinal direction in the green zone, however, chloroplasts in the yellow zone were oval-shaped with thylakoid membranes ranked irregularly. Along with zebra leaves shooting out of the sheath, difference between yellow zone and green zone in the same leaf became more and more evident, in the green zone, thylakoid membranes became rich, and piled up to be the grana. Nevertheless, thylakoid membrane fragmented, internal structure of chloroplast disaggregated seriously and had vesicle structures with high electron density in the yellow zone. With regreening in the yellow zone of zebra leaf, the shape of chloroplast in the yellow zone became spindle, and thylakoid membrane system reconstructed and gradually became rich, orderly arranging along with the long axis of the chloroplast, and some starch granules formed in the chloroplast which indicated that chloroplasts resumed the capacity of photosynthesis.

Key words: Rice, Zebra leaf, Chloroplast, Ultrastructure

[1] Xie R(谢戎), Zhu F-Y(朱发云), He G-H(何光华), Deng X-H(邓锡洪), Zuo Y-S(左永树), Yang Z-L(杨正林), Wu L-J(吴丽君). A preliminary study on the temperature sensitive chlorophyll natural mutant in dual-purpose genic male sterile rice. Southwest China J Agric Sci (西南农业学报), 1995, 8(S1): 124-128 (in Chinese with English abstract)

[2] Kensuke K, Hisayo K, Hikaru S. Characterization of a zebra mutant of rice with increased susceptibility to light stress. Plant Cell Physiol, 2000, 41: 158-164

[3] Shao J-R(邵继荣), Wang Y-Z(王玉忠), Liu Y-S(刘永胜), Sun J-S(孙敬三), Xie R(谢戎). Ultrastructural observation on chloroplast of the thermo-sensitive green-yellow banded leaf mutant in rice (Oryza sativa ssp. indica). Acta Bot Sin (植物学报), 1999, 41(1): 20-24 (in Chinese with English abstract)

[4] Wang Y-Z(王玉忠), Shao J-R(邵继荣), Liu Y-S(刘永胜), Yu J-H(余金洪), Xie R(谢戎), Sun J-S(孙敬三). Alteration of whole leaf protein of a temperature sensitive chlorophyll deficient rice mutant during the bleaching process. Acta Bot Sin (植物学报), 1999, 41(5): 519-523 (in Chinese with English abstract)

[5] He R-F(何瑞锋), Ding Y(丁毅), Yu J-H(余金洪), Zu M-S(祖明生). Study on leaf ultrastructure of the thermo-sensitive chlorophyll deficient mutant in rice. J Wuhan Bot Res (武汉植物学研究), 2001, 19(1): 1-5 (in Chinese with English abstract)

[6] Shao J-R(邵继荣), Sun J-S(孙敬三), Xie R(谢戎). Studies on character of the green-yellow band trait of leaves of temperature sensitive chlorophyll deficient mutant line in rice (Oryza sativa ssp. indica). J Leshan Teachers Coll (乐山师范学院学报), 2003, 18(4): 50-53 (in Chinese with English abstract)

[7] Sun J-S(孙敬三), Zhu Z-Q(朱至清), Wang J-J(王敬驹), Li S-Q(李寿泉). Plastid ultrastructure development and in yellowish mutants of sugar cane. Acta Bot Sin (植物学报), 1981, 23(1): 1-5 (in Chinese with English abstract)

[8] Bachmann M D, Robertson D S, Bowen C C, Anderson I C. Chloroplast development in pigment deficient mutants of maize: I. Structural anomalies in plastids of allelic mutants at the W3 locus. J Ultrastruct Res, 1967, 21: 41-60

[9] Li J G, Li Y X, Geng Y X, Gao W Q. Studies on the chlorophyll-protein plexes and the chloroplast ultrastructure in the barely mutant. Plant Sci Lett, 1983, 30: 312-326

[10] Goodchild D J, Highkin H R, Boardman N K. The fine structure of chloroplast in a barley mutant lacking chlorophyll B. Exp Cell Res, 1966, 43: 684-688

[11] Lin H-H(林宏辉), He L (何礼), Yan Y-C(晏婴才), Dai Q-L(代其林), Du L-F(杜林方), Liang H-G(梁厚果), Tang Z-S(汤泽生), He X-J(何兴金). Studies on structure and function of chloroplast in a chlorophy-less barley mutant. J Sichuan Univ (Nat Sci Edn)(四川大学学报·自然科学版), 2001, 38(6): 899-904 (in Chinese with English abstract)

[12] Dong Z(董遵), Liu J-Y(刘敬阳), Ma H-M(马红梅), Xu C-K(许才康), Sun H(孙华), Zhang J-D(张建栋). Chlorophyll contents and chloroplast ultrastructure of chlorophyll deficient mutant in B. napus. Chin J Oil Crop Sci (中国油料作物学报), 2000, 22(3): 27-29 (in Chinese with English abstract)

[13] Zhao Y(赵云), Du L-F(杜林方), Yang S-H(杨胜洪), Li S-C(李世崇), Zhang Y-Z(张义正). Chloroplast composition and structural differences in a chlorophyll-reduced mutant of oil seed rape seedlings. Acta Bot Sin (植物学报), 2001, 43(8): 877-880 (in Chinese with English abstract)

[14] Zhang D-Y(张道允), Gong Z-Y(巩子英), Ye J-W(叶建伟), Wu Y-X(吴彦霞), Mi H-L(米华玲), Chen G-Y(陈根云). Effects of zebra leaf 1—A new variation mutation-on chloroplast development in rice. J Mol Cell Biol (分子细胞生物学报), 2008, 41(5): 417-422(in English with Chinese abstract)

[15] Wu D-X(吴殿星), Shu Q-R(舒庆尧), Xia Y-W(夏英武), Xia J-F(夏建峰). Study on physiological changes in leaves of a rice greenable albino mutant line W25 during albinism greenism process in late growth period. J Zhejiang Agric Univ (浙江农业大学学报), 1999, 25(1): 1-4 (in Chinese with English abstract)

[16] Su X-J(苏小静), Wang P-H(汪沛洪). Studies on the albescent mechanism in the mutant “stage albinism line of winter wheat”: I. Ultrastructural observation chloroplast during the albescent stage. Acta Univ Agric Boreali-Occident (西北农业大学学报), 1990, 18(2): 73-76 (in Chinese with English abstract)
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