欢迎访问作物学报,今天是

作物学报 ›› 2011, Vol. 37 ›› Issue (02): 348-354.doi: 10.3724/SP.J.1006.2011.00348

• 耕作栽培·生理生化 • 上一篇    下一篇

苎麻耐镉品种差异及其筛选指标分析

佘玮,揭雨成*,邢虎成,鲁雁伟,黄明,康万利,王栋   

  1. 湖南农业大学苎麻研究所 / 湖南省作物种质创新与资源利用重点实验室,湖南长沙 410128
  • 收稿日期:2010-05-26 修回日期:2010-09-21 出版日期:2011-02-12 网络出版日期:2010-11-16
  • 通讯作者: 揭雨成, E-mail: ibfcjyc@ vip.sina.com
  • 基金资助:

    本项目由国家“十一五”科技支撑计划(2008BADA7B02)资助。

Comparison and Screening Indicators for Ramie (Boehmeria nivea) Genotypes Tolerant to Cadmium

SHE Wei,JIE Yu-Cheng*,XING Hu-Cheng,LU Yan-Wei,HUANG Ming,KANG Wan-Li,WANG Dong   

  1. Ramie Institute, Hunan Agricultural University, Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Changsha 410128, China
  • Received:2010-05-26 Revised:2010-09-21 Published:2011-02-12 Published online:2010-11-16
  • Contact: JIE Yu-Cheng, E-mail: ibfcjyc@ vip.sina.com

摘要: 以营养液盆栽试验结合田间微区试验调查9个苎麻品种耐镉差异并筛选耐镉指标。结果表明,营养液盆栽试验中,不同镉处理下苎麻株高、单株叶片数、地上部干重和地下部干重差异显著,各性状具有较大的品种间差异。地上部干重与株高、叶片SPAD值和地下部干重均呈显著或极显著正相关,相关系数分别为0.95、0.82和0.85。田间微区试验中,地上部干重与株高、茎粗、皮厚均呈显著或极显著正相关,相关系数分别为0.86、0.72和0.76。分别对营养液盆栽试验和微区试验各指标耐镉指数的隶属函数值进行聚类分析,9个品种均被分为3类,结果基本一致。说明以营养液盆栽苎麻的株高、叶片SPAD值、地下部干重和地上部干重作为指标筛选苎麻耐镉品种是一条有效途径。

关键词: 苎麻品种, 耐镉, 筛选

Abstract: The rapid industrialization causes the large areas of heavy metal contaminated soils, which has become a threat to the local ecosystems. Cadmium (Cd) is a heavy metal which has aroused great concerns in the environmental science community, because of its toxicity to animals and humans. Screening and breeding high cadmium tolerant ramie genotype is one of the effective alternatives to utilize the cadmium contaminated soil, since ramie could minimize the potential hazard of bringing toxic metals into food chains.In this study, a hydroponic culture experiment and a field micro-plot trial were carried out tocompare nine ramie genotypes and their respective screening indices for Cd tolerance. In the hydroponic culture experiment, substantial differences and significant genotypic variations in plant height, leaf number per plant, shoot dry weight and root dry weight were found in different Cd treatments. The shoot dry weight well correlated with plant height, SPAD of leaf and root dry weight. In the field trial, the shoot dry weight well correlated with plant weight, diameter of stem and thickness of bark. This suggests that these characteristics could be used as Cd tolerance indicators. Cluster analysis using comprehensive Cd tolerance characteristics as evaluation parameters indicated that nine genotypes were divided into three categories. These results suggested that it would probably be an effective method to use the plant height, SPAD of leaf, shoot dry weight and root dry weight in ramie hydroponic culture experiment with different Cd treatments as selection indicators.

Key words: Ramie (Boehmeria nivea), Genotype, Tolerance to Cd, Screening

[1]Li H-F(李花粉). Heavy metals pollution in rhizosphere. J Agric Sci Technol (中国农业科技导报), 2002, 2(4): 54–59(in Chinese with English abstract)
[2]Gouia H, Ghorbal M H, Meyer C. Effects of cadmium on activity of nitrate reductase and on other enzymes of the nitrate assimilation pathway in bean. Plant Physiol Biochem, 2000, 38: 629–638
[3]Kamnev A A, van der Lelie D. Chemical and biological parameters as tools to evaluate and improve heavy metal phytoremediation. Biosci Rep, 2000, 20: 239–258
[4]Gu J-G(顾继光), Zhou Q-X(周启星). Cleaning up through phytoremediation: a review of Cd contaminated soils. Ecol Sci (生态科学), 2002, 21(4): 352–356 (in Chinese with English abstract)
[5]Huang D-F(黄冬芬), Xi L-L(奚岭林), Yang L-N(杨立年), Wang Z-Q(王志琴), Yang J-C(杨建昌). Comparisons in agronomic and physiological traits of rice genotypes differing in cadmium-tolerance. Acta Agron Sin (作物学报), 2008, 34(5): 809–817 (in Chinese with English abstract)
[6]Liu J(刘俊), Liao B-H(廖柏寒), Zhou H(周航), Zhang Y(张永), Zeng M(曾敏), Huang Y-X(黄运湘), Zeng Q-R(曾清如). Main characteristics of physiological-ecological dynamics of soybean during the growth cycle under Cd stress. Acta Ecol Sin (生态学报), 2010, 30(2): 333–340 (in Chinese with English abstract)
[7]Ji S-J(纪淑娟), Wang J-W(王俊伟), Huang L-P(黄莉萍), Lü M(吕美), Wang Y-H(王颜红), Li B(李波), Cui J-H(崔杰华), Wang S-C(王世成). Study on influence of cadmium on growth of Triticum aestivum and forecast of contamination to wheat. Grain Proc (粮食加工), 2008, 33(1): 51–53 (in Chinese with English abstract)
[8]Li J-M(李景梅), Ge J-R(葛建镕), Feng Y-Y(冯耀勇), Niu F-M(牛凤梅), Zhao T-T(赵婷婷), Li C(李程). Effects of different cadmium levels on the growth and nutrient elements in spinach. J Changchun Univ Sci Technol (Nat Sci Edn)(长春理工大学学报), 2007, 30(4): 72–75 (in Chinese with English abstract)
[9]Peng W-Z(彭伟正), Wang K-Q(王克勤), Hu D(胡蝶), Wang C-Y(王彩云). Distribution of Cd in cucumber plant and its effect on growth and some physiological properties. J Agro-Environ Sci (农业环境科学学报), 2006, 25(suppl): 92–95 (in Chinese with English abstract)
[10]Lei M(雷梅), Yue Q-L(岳庆玲), Cheng T-B(陈同斌), Huang Z-C(黄泽春), Liao X-Y(廖晓勇), Liu Y-R(刘颖茹), Zheng G-D(郑国砥), Chuang Q-R(常庆瑞). Heavy metal concentrations in soils and plants around Shizhuyuan mining area of Hunan Province. Acta Ecol Sin (生态学报), 2005, 25(5): 1146–1151 (in Chinese with English abstract)
[11]She W(佘玮), Jie Y-C(揭雨成), Xing H-C(邢虎成), Huang M(黄明), Kang W-L(康万利), Lu Y-W(鲁雁伟), Wang D(王栋). Uptake and accumulation of heavy metal by ramie (Boehmeria nivea) growing on antimony mining area in Lengshuijiang City of Hunan Province. J Agro-Environ Sci (农业环境科学学报), 2010, 29(1): 91–96 (in Chinese with English abstract)
[12]Jie Y-C(揭雨成), Leng J(冷鹃). Research on ramie germplasm resource of China. Chin Fiber Crops (中国麻作), 2000, 22(3): 13–15 (in Chinese with English abstract)
[13]Cao D-J(曹德菊), Zhou S-B(周世杯), Xiang J(项剑). Ramie tolerance to Cd in soil and its accumulation effect. Chin Fiber Prod (中国麻业), 2004, 26(6): 271–274 (in Chinese with English abstract)
[14]Dai J-P(代剑平), Jie Y-C(揭雨成), Leng J(冷鹃), Sun Z-M(孙志民). Study on the cadmium distributing regulation in different parts of plant of different ramie germplasms. Chin Fiber Prod (中国麻业), 2003, 25(6): 279–293 (in Chinese with English abstract)
[15]Xu Y(许英), Dai J-P(代剑平), Jie Y-C(揭雨成), Sun Z-M(孙志民), Chen J-F(陈建芳), Wang X-F(王晓飞). Physiological response of ramie under Cd stress and Cd resistance. Plant Fibers Sci Chin (中国麻业科学), 2006, 28(6): 301–305 (in Chinese with English abstract)
[16]Rout G R, Samantaray S, Das P. Differential cadmium tolerance of mung bean and rice cultivars in hydroponic culture. Acta Agric Scandinavica(B), Soil Plant Sci, 2000, 49: 234–241
[17]Wang S-L(王松良), Chen X-Y(陈选阳), Chen H(陈辉), Chen D-M(陈冬梅), Wang F-J(王峰吉), Gao W-X(高文霞), Zhang Z-J(张志坚), Wang J-M(王建明). Physio-chemical mechanism of cadmium tolerance of Chinese cabbage (Brassica chinensis). Chin J Eco-Agric (中国生态农业学报), 2007, 15(5): 120–124 (in Chinese with English abstract)
[18]SanitÂdi Toppi L, Gabbrielli R. Response to cadmium in higher plants. Environ Exp Bot, 1999, 41: 105–130
[19]Singh B R, Narwal R P, Jeng A S, Almas A. Crop uptake and extractability of cadmiun in soils naturally high inmetals at different pH levels. Soil Sci Plant An, 1995, 26: 2123–2142
[20]Ye Z H, Baker A J M, Wong M H, Willis A J. Zinc, lead and cadmium tolerance, uptake and accumulation by the common reed, Phragmitesaust ralis (Gav.) Trin. ex Steudel. Annals Bot, 1997, 80: 363–370
[21]Wang X(王欣), Liu Y-G(刘云国), Aibibu·Nuzhaaiti(艾比布·努扎艾提), Zhang D-M(张东梅), Xu W-H(徐卫华), Zhou M(周鸣), Chai L-Y(柴立元). Endurance mechanism of ramie to Cd and the alleviating effect of exogenous spermine. J Agro-Environ Sci (农业环境科学学报), 2007, 26(2): 487–493 (in Chinese with English abstract)
[22]Yang B, Zhou M, Shu W S, Lan C Y, Ye Z H, Qiu R L, Jie Y C, Cui G X, Wong M H. Constitutional tolerance to heavy metals of a fiber crop, ramie (Boehmeria nivea), and its potential usage. Environ Poll, 2010, 158: 551–558
[23]Tian X-L(田晓莉), Wang G-W(王刚卫), Zhu R(朱睿), Yang P-Z(杨培珠), Duan L-S(段留生), Li Z-H(李召虎). Conditions and Indicators for screening cotton (Gossypium hirsutum) genotypes tolerant to low-potassium. Acta Agron Sin (作物学报), 2008, 34(8): 1435–1443(in Chinese with English abstract)
[24]Abedin M J, Meharg A A. Relative toxicity of arsenite and arsenate on germination and early seedling growth of rice (O ryza sativa L.). Plant Soil, 2002, 243: 57–66
[25]Wang C-C(王春春), Shen Z-G(沈振国). Uptake of Cd by three species of plants and responses of mung bean to Cd toxicity. J Nanjing Agric Univ (南京农业大学学报), 2001, 24(4): 9–13 (in Chinese with English abstract)
[26]Chen C-M(陈朝明), Gong H-Q(龚惠群). Effect of Cd on quality, physiological and biochemical characteristics of mulberry leaves and its mechanism. Chin J Appl Ecol (应用生态学报), 1996, 7(4): 417–423 (in Chinese with English abstract)
[27]Tang Y-T(汤叶涛), Guan L-J(关丽捷), Qiu R-L(仇荣亮), Ying R-R(应蓉蓉), Liu F-J(刘凤杰), Hu P-J(胡鹏杰). Antioxidative defense to cadmium in hyperaccumulator Picris divaricata V. Acta Ecol Sin (生态学报), 2010, 30(2): 324–332 (in Chinese with English abstract)
[28]Yu F-M(于方明), Tang Y-T(汤叶涛), Qiu R-L(仇荣亮), Zhou X-Y(周小勇), Ying R-R(应蓉蓉), Hu P-J(胡鹏杰), Zhang T(张涛). Antioxidative responses to cadmium stress in the hyperaccumulator Arabis paniculata Franch. Acta Sci Circumstantiae (环境科学学报), 2010, 30(2): 409–414 (in Chinese with English abstract)
[1] 秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[2] 张鹤, 蒋春姬, 殷冬梅, 董佳乐, 任婧瑶, 赵新华, 钟超, 王晓光, 于海秋. 花生耐冷综合评价体系构建及耐冷种质筛选[J]. 作物学报, 2021, 47(9): 1753-1767.
[3] 王南,祁显涛,刘昌林,谢传晓,朱金洁. 基于CRISPR/Cas9核糖核蛋白体DNA定点内切酶体外活性建立高效基因型分析技术[J]. 作物学报, 2020, 46(7): 978-986.
[4] 董庆园,马德清,杨学,刘勇,黄昌军,袁诚,方敦煌,于海芹,童治军,沈俊儒,许银莲,罗美中,李永平,曾建敏. 高抗黑胫病烤烟BAC文库的构建及分析[J]. 作物学报, 2020, 46(6): 869-877.
[5] 王瑞莉,王刘艳,叶桑,郜欢欢,雷维,吴家怡,袁芳,孟丽姣,唐章林,李加纳,周清元,崔翠. 铝毒胁迫下甘蓝型油菜种子萌发期相关性状的QTL定位[J]. 作物学报, 2020, 46(6): 832-843.
[6] 卫平洋,裘实,唐健,肖丹丹,朱盈,刘国栋,邢志鹏,胡雅杰,郭保卫,高尚勤,魏海燕,张洪程. 安徽沿淮地区优质高产常规粳稻品种筛选及特征特性[J]. 作物学报, 2020, 46(4): 571-585.
[7] 霍强,杨鸿,陈志友,荐红举,曲存民,卢坤,李加纳. 基于QTL定位和全基因组关联分析筛选甘蓝型油菜株高和一次有效分枝高度的候选基因[J]. 作物学报, 2020, 46(02): 214-227.
[8] 郜欢欢,叶桑,王倩,王刘艳,王瑞莉,陈柳依,唐章林,李加纳,周清元,崔翠. 甘蓝型油菜种子萌发期耐铝毒特性综合评价及其种质筛选[J]. 作物学报, 2019, 45(9): 1416-1430.
[9] 张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰. 基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析[J]. 作物学报, 2019, 45(5): 683-692.
[10] 崔翠,程闯,赵愉风,郜欢欢,王瑞莉,王刘艳,周清元. 52份豌豆种质萌发期耐铝毒性的综合评价与筛选[J]. 作物学报, 2019, 45(5): 798-805.
[11] 查象敏, 汪海, 路小铎, 张春义, 黄大昉, 郎志宏. 玉米茉莉酸甲酯不敏感突变体的筛选[J]. 作物学报, 2015, 41(09): 1454-1461.
[12] 董军刚,董振生,孟倩,张博. 甘蓝型油菜抗裂角材料资源的筛选[J]. 作物学报, 2014, 40(12): 2203-2209.
[13] 唐忠厚,张允刚,魏猛,陈晓光,史新敏,张爱君,李洪民,丁艳锋. 耐低钾和钾高效型甘薯品种(系)的筛选及评价指标[J]. 作物学报, 2014, 40(03): 542-549.
[14] 张晓霞, 焦浈, 董振营, 李世明, 王燃, 凌宏清, 秦广雍, 王道文. 普通小麦品种小偃54中α/β-醇溶蛋白编码基因的克隆与序列分析[J]. 作物学报, 2011, 37(08): 1497-1502.
[15] 王宝祥,江玲,陈亮明,卢百关,王琦,黎光泉,樊继伟,程遐年,翟虎渠,徐大勇,万建民. 水稻黑条矮缩病抗性资源的筛选和抗性QTL 的定位[J]. 作物学报, 2010, 36(08): 1258-1264.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!