欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2011, Vol. 37 ›› Issue (06): 975-981.doi: 10.3724/SP.J.1006.2011.00975

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用分子标记辅助选择聚合水稻抗病基因Pi-ta、Pi-b和Stv-bi

王军, 杨杰, 陈志德, 范方军, 朱金燕, 杨金欢, 仲维功*   

  1. 江苏省农业科学院粮食作物研究所 / 国家水稻改良中心南京分中心，江苏南京210014
  • 收稿日期:2010-11-05 修回日期:2011-03-28 出版日期:2011-06-12 网络出版日期:2011-04-12
  • 通讯作者: 仲维功, E-mail: wgzhong0503@yahoo.com.cn
  • 基金资助:

    本研究由江苏省农业科技自主创新基金项目(cx[10]402)，国家科技支撑计划重大项目(2006BADO2A03)，江苏省科技支撑计划项目(BE2009301-2)，国家转基因生物新品种培育科技重大专项(2009ZX08001-005B, 019)和江苏省自然科学基金项目(BK2009321)资助。

Pyramiding Resistance Gene Pi-ta, Pi-b, and Stv-bi by Marker-assisted Selection in Rice (Oryza sativa L.)

WANG Jun,YANG Jie,CHEN Zhi-De,FAN Fang-Jun,ZHU Jin-Yan,YANG Jin-Huan,ZHONG Wei-Gong*   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences / Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China
  • Received:2010-11-05 Revised:2011-03-28 Published:2011-06-12 Published online:2011-04-12
  • Contact: 仲维功, E-mail: wgzhong0503@yahoo.com.cn

PDF

2195

被引次数

47

摘要： 水稻稻瘟病和条纹叶枯病是长江中下游粳稻稻区两大主要病害，选育抗病品种是防治这两大病害最有效的方法。以同时含有稻瘟病抗病基因Pi-ta和Pi-b的武运粳8号，含有条纹叶枯病抗病基因Stv-bi的镇稻42为基因供体配置杂交组合。利用Pi-ta和Pi-b的基因标记和Stv-bi紧密连锁的分子标记对分离世代进行基因位点的检测，结合田间多代选育、抗性鉴定将3个抗病基因同时转育到高产品种中，选育出高产、优质、多抗水稻新品系74121。利用分子标记辅助选择，为选育多抗水稻新品种提供了一种简单、快捷的选择方法，同时也为水稻抗病育种提供了新的遗传资源。

关键词: 水稻, 稻瘟病, 条纹叶枯病, Pi-ta, Pi-b, Stv-bi, 分子标记辅助选择

Abstract: The blast and stripe disease are two major diseases of rice in Yangtze River japonica cultivating area. Breeding resistant cultivars is the most effective way to prevent these two major diseases. Wuyujing 8, containing both Pi-ta and Pi-b, was crossed with Zhendao 42 which carried rice stripe disease resistance gene Stv-bi. Two gene-markers, Pita and Pib, and one SCAR marker tightly linked with Stv-bi were used for marker-assisted selection in each segregating generation. After the multi-generation breeding and identification of resistance, three resistance genes were put together finally. One new rice stable line, 74121 was obtained which conferred good agronomic characteristics containing high-yield, good quality and multi-resistance. These results indicated that marker-assisted selection could not only be a simple and effective way but also provide genetic resources for breeding new rice multi-resistant varieties.

Key words: Rice, Blast, Stripe disease, Pi-ta, Pi-b, Stv-bi, Marker-assisted selection

[1]Wang C-L(王才林), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Chen T(陈涛). Rice breeding for resistance to stripe virus disease. Acta Agron Sin (作物学报), 2008, 34(3): 530–533 (in Chinese with English abstract)
[2]Ou S H. Rice Diseases, 2nd edn. UK: CAB International, 1985. pp 109–201
[3]Bryan G T, Wu K S, Farrall L, Jia Y L, Hershey H P, McAdams S A, Faulk K N, Donaldson G K, Tarchini R, Valent B. A single amino acid difference distinguishes resistant ansusceptible alleles of the rice blast resistance gene Pi-ta. Plant Cell, 2000, 12: 2033–2046
[4]Wang Z X, Yano M, Yamanouchi U, Iwamoto M, Monna1 L, Hayasaka H, Katayose Y, Sasaki T. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes. Plant J, 1999, 19: 55–64
[5]Yao S(姚姝), Chen T(陈涛), Zhang Y-D(张亚东), Zhu Z(朱镇), Zhao L(赵凌), Zhao Q-Y(赵庆勇), Zhou L-H(周丽慧), Wang C-L(王才林). Pyramiding of translucent endosperm mutant gene Wx-mq and rice stripe disease resistance gene, Stv-bi by marker-assisted selection in rice (Oryza sativa). Chin J Rice Sci (中国水稻科学), 2010, 24(4): 341–347 (in Chinese with English abstract)
[6]Hayano-Saito Y, Tsuji T, Fujii K, Saito K, Iwasaki M, Saito A. Localization of the rice stripe disease resistance gene, Stv-bi, by graphical genotyping and linkage analyses with molecular markers. Theor Appl Genet, 1998, 96: 1044–1049
[7]Hayano-Saito Y, Saito K, Nakamura S, Kawasaki S, Iwasaki M. Fine physical mapping of the rice stripe resistance gene locus, Stv-bi. Theor Appl Genet, 2000, 101: 59-63
[8]Hayano-Saito Y, Saito K, Fujii K, Touyama T, Tsuji T, Sugiura N, Izawa T, Iwasaki M. SCAR marker for selection of the rice stripe resistance gene Stv-bi. J Breed Res, 2000, 2: 67–72
[9]Luo C-P(罗楚平), Ni L(倪磊), Chen Z-Y(陈志谊), Liu Y-F(刘永锋), Liu Y-Z(刘邮洲), Nie Y-F(聂亚锋). Inoculation technology of rice blast and rice resistance to it in Jiangsu regional tests in 2009. Jiangsu Agric Sci (江苏农业科学), 2009, (6): 178–179 (in Chinese)
[10]Zhou T(周彤), Zhou Y-J(周益军), Cheng Z-B(程兆榜), Yang R-M(杨荣明), Ji J-A(吉健安). Identification of resistance to rice stripe virus in japonica rice varieties and analysis for its inhertance of Zhendao 88. Acta Phytophyl Sin (植物保护学报), 2007, 34(5): 475–479 (in Chinese with English abstract)
[11]Wang Z-H(王忠华), Jia Y-L(贾育林), Wu D-X(吴殿星), Xia Y-W(夏英武). Molecular markers-assisted selection of the rice blast resistance gene Pi-ta. Acta Agron Sin (作物学报), 2004, 30(12): 1259–1265 (in Chinese with English abstract)
[12]Fjellstrom R, Conaway-Bormans C A, McClung A M, Marchetti M A, Shank A R, Park W D. Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes. Crop Sci, 2004, 44: 1790–1798
[13]Wang J(王军), Yang J(杨杰), Chen Z-D(陈志德), Zhong W-G(仲维功). Development and application of fragrance gene markers in rice. Mol Plant Breed (分子植物育种), 2008, 6(6): 1209–1212 (in Chinese with English abstract)
[14]Lei C-L(雷财林), Ling Z-Z(凌忠专), Wang J-L(王久林), Wan J-M(万建民). Research advanccs in rice breeding for disease resistance. Bull Biol (生物学通报), 2004, 39(11): 4–7 (in Chinese)
[15]Chen H-Q(陈红旗), Chen Z-Q(陈宗祥), Ni S(倪深), Zuo S-M(左示敏), Pan X-B(潘学彪), Zhu X-D(朱旭东). Pyramiding three genes with resistance to blast by marker-assisted selection to improve rice blast resistance of Jin23B. Chin J Rice Sci (中国水稻科学), 2008, 22(1): 23–27 (in Chinese with English abstract)
[16]Chen X-W(陈学伟), Li S-G(李仕贵), Ma Y-Q(马玉清), Li H-Y(黎汉云), Zhou K-D(周开达), Zhu L-H(朱立煌). Marker-assisted selection and pyramiding for three blast resistance genes, Pi-d(t)1, Pi-b, Pi-ta2, in rice. Chin J Biotech (生物工程学报), 2004, 20(5):708–713 (in Chinese with English abstract)
[17]Yoshimura S, Yoshimura A, Iwata N, McCouch S R. Tagging and combining bacterial blight resistance gene in rice using RAPD and RFLP markers. Mol Breed, 1995, 1: 375–387
[18]Huang N, Angeles E R, Domingo J, Magpantay G, Singh S, Zhang G, Kumaravadivel N, Bennett J, Khush G S. Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theor Appl Genet, 1997, 95: 313–320
[19]Deng Q-M(邓其明), Zhou Y-J(周宇爝), Jiang Z-X(蒋昭雪), Wan Y-X(万映秀), Zhao B(赵斌), Yang L(杨丽), Li P(李平). Pyramiding bacterial blight (BB) resistance genes (Xa21, Xa4 and Xa23) into rice and its effect analysis. Acta Agron Sin (作物学报), 2005, 31(9): 1241−1246 (in Chinese with English abstract)
[20]Domingo J P, New T W, Kumaravaclivel N G, Magpantay G B, Hittalmani S, Parco A S, Huang N, Angeles E R, Khush G S. Rice resistant breeding to bacterial blight and blast by molecular marker-assisted selection. Philippine J Crop Sci, 1995, 20: 3
[21]Narayanar N N, Baisakh N, Vera Cruz C M, Gnanamanickam S S, Datta K, Datta S K. Improvement rice resistance to bacterial blight and blast through molecular breeding. Crop Sci, 2002, 42: 2072–2079
[22]Ni D-H(倪大虎), Yi C-X(易成新), Li L(李丽), Wang X-F(汪秀峰), Zhang Y(张毅), Zhao K-J(赵开军), Wang C-L(王春连), Zhang Q(章琦), Wang W-X(王文相), Yang J-B(杨剑波). Developing rice lines resistant to bacterial blight and blast with molecular marker-assisted selection. Acta Agron Sin (作物学报), 2008, 34(1): 100–105 (in Chinese with English abstract)
[23]Sugiura N, Tsuji T, Fujii K, Kato T, Saka N, Touyama T, Hayano-Saito Y, Izawa T. Molecular marker-assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.). Breed Res, 2004, 6: 143–148 (in Japanese with English abstract)
[1] 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[2] 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742.
[3] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[4] 陈伟, 卫万娟, 赵其兵, 常东伟, 余凌波, 翟鹏飞, 冯志明, 陈宗祥, 任仰涛, 杨鹏, 刘海浪, 李珍富, 杨永乐, 金彦刚, 左示敏. 利用CRISPR/Cas9编辑Hd6基因创制优质早熟水稻新种质[J]. 作物学报, 2026, 52(4): 1046-1056.
[5] 石少阶, 刘凯, 陈姿夷, 王卉颖, 李三和, 周雷, 游艾青. 水稻矮化多分蘖基因DMT1的克隆与功能分析[J]. 作物学报, 2026, 52(4): 1022-1034.
[6] 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812.
[7] 叶凡, 李帅, 李思宇, 陈云, 窦超银, 刘立军. 不同节水灌溉方式对东北稻区水稻产量和群体质量的影响[J]. 作物学报, 2026, 52(3): 895-907.
[8] 王婵, 吴莹莹, 李文奇, 李霞, 王芳权, 周彤, 杨杰. 基于HRM技术开发水稻抗条纹叶枯病基因STV11功能标记[J]. 作物学报, 2025, 51(9): 2547-2556.
[9] 陈惠莹, 何嘉欣, 朱斌, 黄士轩, 周星佑, 伍君权, 杨美艳. 水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究[J]. 作物学报, 2025, 51(8): 2087-2099.
[10] 杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724.
[11] 凤舞剑, 冼晓青, 张新钵, 曹丹, 强承魁. 基于转录组和AlphaFold对稻瘟菌经典效应蛋白和水稻受体的快速鉴定[J]. 作物学报, 2025, 51(6): 1480-1488.
[12] 雷松翰, 范骏扬, 车艳奕, 代永东, 郑雨萌, 田维江, 桑贤春, 王晓雯. 水稻内卷叶突变体acl3的鉴定及调控基因的功能分析[J]. 作物学报, 2025, 51(6): 1467-1479.
[13] 李福媛, 杨奕, 马继琼, 许明辉, 林良斌, 孙一丁. 水稻OsPUB4基因克隆、激素诱导表达分析与互作蛋白筛选[J]. 作物学报, 2025, 51(6): 1690-1700.
[14] 王梦宁, 谢可冉, 高逖, 王飞, 任孝俭, 熊栋梁, 黄见良, 彭少兵, 崔克辉. 水稻幼穗分化期至抽穗期高温对籽粒形态和充实的影响及其与粒重的关系[J]. 作物学报, 2025, 51(5): 1347-1362.
[15] 盛倩男, 方娅婷, 赵剑, 杜思垚, 胡行珍, 余秋华, 朱俊, 任涛, 鲁剑巍. 不同养分管理措施对稻田和旱地油菜产量的影响及其对冻害的响应[J]. 作物学报, 2025, 51(5): 1286-1298.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2