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作物学报 ›› 2011, Vol. 37 ›› Issue (07): 1212-1218.doi: 10.3724/SP.J.1006.2011.01212

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米异交不亲和基因Ga1-S的蛋白质组分析

刘怀华,王莉雯**,刘旭,马侠,宁丽华,张华,崔德周,姜川,陈化榜*   

  1. 山东农业大学农学院 / 作物生物学国家重点实验室 / 山东省作物生物学重点实验室，山东泰安271018
  • 收稿日期:2010-11-16 修回日期:2011-03-28 出版日期:2011-07-12 网络出版日期:2011-04-12
  • 通讯作者: 陈化榜, E-mail: hbchen@sdau.edu.cn, Tel: 0538-8249939
  • 基金资助:

    本研究由国家自然科学基金项目(30971788), 中国科学院所级领域前沿探索项目和山东省农业良种重大课题(鲁农良种字2008-6)资助。

Proteomic Analyses of the Maize Cross Incompatibility Gene Ga1-S

LIU Huai-Hua,WANG Li-Wen**,LIU Xu,MA Xia,NING Li-Hua,ZHANG Hua,CUI De-Zhou,JIANG Chuan,CHEN Hua-Bang*   

  1. State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / Agronomy College, Shandong Agricultural University, Tai’an 271018, China
  • Received:2010-11-16 Revised:2011-03-28 Published:2011-07-12 Published online:2011-04-12
  • Contact: 陈化榜, E-mail: hbchen@sdau.edu.cn, Tel: 0538-8249939

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1658

被引次数

7

摘要： 为了研究玉米异交不亲和的蛋白表达机制，以玉米(Zea mays L.)异交不亲和基因Gal-S的一对近等基因系W22(GG)与w22(gg)为材料，组配自交(GG×GG)、正交(gg×GG)与反交(GG×gg) 3个组合。首先采用荧光显微技术, 观察比较了3个组合中花粉管在花丝中的生长过程；其次采用IEF/SDS-PAGE双向凝胶电泳与基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术，比较研究了授粉后10 h自交与反交母本W22(GG)花丝蛋白质组的特异表达差异。结果表明，授粉后10 h，自交与正交花粉管伸长可达花丝基部，而反交花丝基部未观察到花粉管；自交与反交母本W22(GG)花丝蛋白质组共检测到25个差异蛋白质点，其中15个在自交中特异表达，10个在反交中特异表达；通过MALDI-TOF-MS质谱测序和MASCOT序列分析，注释了12个蛋白质点；其中自交中特异表达的蛋白质点11、12、14和异交中特异表达的蛋白质点18、22、24可能与玉米异交不亲和密切相关。

关键词: 玉米, 异交不亲和, Ga1-S, 蛋白质组, 双向电泳, 质谱分析

Abstract: The objective of this paper was to study the cross incompatibility gene Ga1-S in maize through proteomic approach. Near isogonic lines of Ga1-S gene, W22 (GG) and w22 (gg), were used to make the crosses of GG×GG, gg×GG,and GG×gg respectively. First，we observed the growth process of pollen tubes grown into silks in three crosses by fluorescence microscopy; second, total silk proteins were extracted from silks of the W22 (GG) at 10 h after pollination. Total proteins were extracted by TCA/Acetone, separated by two-dimensional gel electrophoresis (2-DE) and analyzed through MALDI-TOF-MS mass spectrometry. The results indicated that pollen tube couldn’t grow into silks base in the cross of GG×gg, but could in the other two crosses; In the silk proteome of GG×GG and GG×gg , there were 25 differentially expressed proteins, including 15 specifically expressed in GG×GG, and 10 specifically expressed in GG×gg. And 12 of them were annotated in various databases by MALDI-TOF-MS and MASCOT analyses. Proteins 11, 12, 14, 18, 22 and 24 presumably play important roles in the maize cross incompatibility.

Key words: Zea mays L., Cross incompatibility, Ga1-S, Proteome, 2-DE, MALDI-TOF-MS

[1]Schwarts D. The analysis of case of cross sterility in maize. Proc Natl Acad Sci USA, 1950, 36: 719–724
[2]Lino De la C L, Jose de J S G. The gametophyte factor1(Ga1) in Mexican commercial hybrids of maize. Rev Fitotec Mex, 2008, 31: 57–65
[3]Nelson O E. The gametophyte factors of maize. In: Freeling M, Walbot V (editors) The Maize Handbook. New York, Inc: Springer-Verlag, 1994. pp 496–503
[4]Ashman R B. Modification of cross-sterility in maize. J Hered, 1975, 66: 5–9
[5]Kermicle J L, Allen J O. Cross incompatibility between maize and teosinte. Maydica, 1990, 35: 399–408
[6]Andreas L, Irina K, Kanok-orn S, Thomas D. Sporophytic control of pollen tube growth and guidance in maize. J Exp Bot, 2010, 61(3): 673–682
[7]Kermicle J L, Evans M M S. Pollen-pistil barriers to crossing in maize and teosinte result from incongruity rather than active rejection. Sexual Plant Reproduction, 2005, 8: 187–194
[8]Kho Y O, Baer J. Observing pollen tubes by means of fluorescence. Euphytica, 1968, 17: 298–302
[9]2-D Electrophoresis using immobilized pH gradients: Principles and Methods. A Laboratory Manual. Amersham Biosciences, 80-6429-60
[10]Von Besser K, Frank A C, Johnson M A, Preuss D. Arabidopsis HAP2 (GCS1) is a sperm-specific gene required for pollen tube guidance and fertilization. Development, 2006, 133: 4761–4769
[11]Galinha C, Bilsborough G, Tsiantis M. Hormonal input in plant meristems: a balancing act. Seminars in Cell & Developmental Biology, 2009, 20: 1149–1156
[12]Duan T-L(段桃利), Mu J-Y(牟锦毅), Tang Q-L(唐祈林), Rong T-Z(荣廷昭), Wang P(王培). Sexual incompatibility between maize and its wild relatives Tripsacum L. and Coix L. Acta Agron Sin (作物学报), 2008, 34(9): 1656–1661 (in Chinese with English abstract)
[13]Wen L, Liu G, Li S Q. Proteomic analysis of anthers from Honglian cytoplasmic male sterility line rice and its corresponding maintainer and hybrid. Botanical Studies, 2007, 48: 293–309
[14]Bitto E, Bingman C A, McCoy J G, Allard S T M, Wesenberg G E, Phillips Jr G N. The structure at 1.6 A resolution of the protein product of the At4g34215 gene from Arabidopsis thaliana. Acta Crystallographica Section D, 2005, 61: 1655–1661
[15]Nielsen H, Engelbrecht J, Brunak S, von Heijne G. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Engineering, 1997, 10: 1–6
[16]Shaw J F, Chang R C, Chuang K H, Yen Y T, Wang F G.. Nucleotide sequence of a novel arylesterase gene from Vibriomimicus and characterization of the enzyme expressed in Escherichia coli. Biochem J, 1994, 298: 675–680
[17]Dalrymple B P, Cybinski D H, Layton I, McSweeney C S, Xue G P, Swading Y J, Lowry J B. Three Neocallimastix patriciarum esterases associated with the degradation of complex polysaccharides are members of a new family of hydrolases. Microbiology, 1997, 143: 2605–2614
[18]Molgaard A, Kauppinen S, Larsen S. Rhamnogalacturonan acetylesterase elucidates the structure and function of a new family of hydrolases. Structure with Fold and Design, 2000, 8: 373–338
[19]Lo Y C, Lin S C, Shaw J F, Liaw Y C. Crystal structure of the Escherichia coli thioesterase I/protease I/lysophospholipase L1: consensus sequence blocks constitute the catalytic center of SGNH-hydrolases through a conserved hydrogen bond network. J Mol Biol, 2003, 330: 539–551
[20]Reina J J, Guerrero C, Heredia A. Isolation, characterization, and localization of AgaSGNH cDNA: a new SGNH-motif plant hydrolase specic to Agave Americana L. leafe pidermis. J Exp Bot, 2007, 58: 2712–2731
[21]Updegraff E P, Zhao F, Preuss D. The extracellular lipase EXL4 is required for efficient hydration of Arabidopsis pollen. Sex Plant Reprod, 2009, 22: 197–204
[22]Li H, Xue D, Gao Z, Yan M, Xu W, Xing Z, Huang D, Qian Q, Xue Y. A putative lipase gene EXTRA GLUME1 regulates both empty-glume fate and spikelet development in rice. Plant J, 2009, 57: 593–605
[23]Arif S A, Hamilton R G, Yusof F, Chew N P, Loke Y H, Nimkar S, Beintema J J, Yeang H Y. Isolation and characterization of the early nodule-especific protein homologue (Hev b13), an allergenic lipolytic esterase from Hevea brasiliensis latex. J Biol Chem, 2004, 279: 23933–23941
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