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作物学报 ›› 2011, Vol. 37 ›› Issue (07): 1212-1218.doi: 10.3724/SP.J.1006.2011.01212

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米异交不亲和基因Ga1-S的蛋白质组分析

刘怀华,王莉雯**,刘旭,马侠,宁丽华,张华,崔德周,姜川,陈化榜*   

  1. 山东农业大学农学院 / 作物生物学国家重点实验室 / 山东省作物生物学重点实验室，山东泰安271018
  • 收稿日期:2010-11-16 修回日期:2011-03-28 出版日期:2011-07-12 网络出版日期:2011-04-12
  • 通讯作者: 陈化榜, E-mail: hbchen@sdau.edu.cn, Tel: 0538-8249939
  • 基金资助:

    本研究由国家自然科学基金项目(30971788), 中国科学院所级领域前沿探索项目和山东省农业良种重大课题(鲁农良种字2008-6)资助。

Proteomic Analyses of the Maize Cross Incompatibility Gene Ga1-S

LIU Huai-Hua,WANG Li-Wen**,LIU Xu,MA Xia,NING Li-Hua,ZHANG Hua,CUI De-Zhou,JIANG Chuan,CHEN Hua-Bang*   

  1. State Key Laboratory of Crop Biology / Shandong Key Laboratory of Crop Biology / Agronomy College, Shandong Agricultural University, Tai’an 271018, China
  • Received:2010-11-16 Revised:2011-03-28 Published:2011-07-12 Published online:2011-04-12
  • Contact: 陈化榜, E-mail: hbchen@sdau.edu.cn, Tel: 0538-8249939

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1825

被引次数

7

摘要： 为了研究玉米异交不亲和的蛋白表达机制，以玉米(Zea mays L.)异交不亲和基因Gal-S的一对近等基因系W22(GG)与w22(gg)为材料，组配自交(GG×GG)、正交(gg×GG)与反交(GG×gg) 3个组合。首先采用荧光显微技术, 观察比较了3个组合中花粉管在花丝中的生长过程；其次采用IEF/SDS-PAGE双向凝胶电泳与基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术，比较研究了授粉后10 h自交与反交母本W22(GG)花丝蛋白质组的特异表达差异。结果表明，授粉后10 h，自交与正交花粉管伸长可达花丝基部，而反交花丝基部未观察到花粉管；自交与反交母本W22(GG)花丝蛋白质组共检测到25个差异蛋白质点，其中15个在自交中特异表达，10个在反交中特异表达；通过MALDI-TOF-MS质谱测序和MASCOT序列分析，注释了12个蛋白质点；其中自交中特异表达的蛋白质点11、12、14和异交中特异表达的蛋白质点18、22、24可能与玉米异交不亲和密切相关。

关键词: 玉米, 异交不亲和, Ga1-S, 蛋白质组, 双向电泳, 质谱分析

Abstract: The objective of this paper was to study the cross incompatibility gene Ga1-S in maize through proteomic approach. Near isogonic lines of Ga1-S gene, W22 (GG) and w22 (gg), were used to make the crosses of GG×GG, gg×GG,and GG×gg respectively. First，we observed the growth process of pollen tubes grown into silks in three crosses by fluorescence microscopy; second, total silk proteins were extracted from silks of the W22 (GG) at 10 h after pollination. Total proteins were extracted by TCA/Acetone, separated by two-dimensional gel electrophoresis (2-DE) and analyzed through MALDI-TOF-MS mass spectrometry. The results indicated that pollen tube couldn’t grow into silks base in the cross of GG×gg, but could in the other two crosses; In the silk proteome of GG×GG and GG×gg , there were 25 differentially expressed proteins, including 15 specifically expressed in GG×GG, and 10 specifically expressed in GG×gg. And 12 of them were annotated in various databases by MALDI-TOF-MS and MASCOT analyses. Proteins 11, 12, 14, 18, 22 and 24 presumably play important roles in the maize cross incompatibility.

Key words: Zea mays L., Cross incompatibility, Ga1-S, Proteome, 2-DE, MALDI-TOF-MS

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