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作物学报 ›› 2011, Vol. 37 ›› Issue (10): 1779-1784.doi: 10.3724/SP.J.1006.2011.01771

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

一个水稻多柱头突变体的形态特征和基因定位

周明镜1,2，文勇2，李双成1,2，李成波2，张曼华2，高烽焱2，王玲霞1,2，李平1,2,*   

  1. 1四川农业大学水稻研究所, 四川温江611130；2四川农业大学 / 作物基因资源与遗传改良教育部重点实验室, 四川雅安625014
  • 收稿日期:2011-01-25 修回日期:2011-06-25 出版日期:2011-10-12 网络出版日期:2011-07-28
  • 通讯作者: 李平, E-mail: liping6575@163.com
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2011CB100101)和国家自然科学基金项目(30800084)资助。

Phenotypic Characterization and Genetic Mapping of an Increased Stigma Mutant in Rice (Oryza sativa L.)

ZHOU Ming-Jing1,2,WEN Yong2,LI Shuang-Cheng1,2,LI Cheng-Bo2,ZHANG Man-Hua2,GAO Feng-Yan2,WANG Ling-Xia1,2,LI Ping1,2,*   

  1. 1Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; 2 Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education / Sichuan Agricultural University, Ya’an 625014, China
  • Received:2011-01-25 Revised:2011-06-25 Published:2011-10-12 Published online:2011-07-28
  • Contact: 李平, E-mail: liping6575@163.com

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摘要： 水稻产量和品质受花器官发育的直接影响, 因此对水稻颖花发育机理的研究将有助于水稻产量和品质的遗传改良。在籼稻C2与2480的杂交后代中发现了一个多柱头突变体, 与野生型相比, 该突变体植株矮化、穗变小、开花延迟和育性降低。颖花解剖发现, 其浆片正常, 柱头和雌蕊数量增加, 雄蕊数目明显减少, 并伴随不同程度的雌雄蕊畸形。以突变体作母本, 构建群体进行遗传分析, 结果显示所有F1均表现正常, F2群体出现3∶1性状分离, 证实该突变性状受1对隐性基因控制。利用微卫星标记进行连锁分析, 将该基因定位于水稻第6染色体上标记RM3183和RM3827之间, 遗传距离分别为2.2 cM和12.0 cM, 且与RM11951、RM19953和RM19961共分离。ISM(t)是一个新的水稻花器官发育控制基因, 本研究为该基因的克隆和功能研究奠定了基础。

关键词: 水稻, 花器官, 突变体, 遗传分析, 基因定位

Abstract: Floral organ development directly affects rice yield and quality. Here we report a rice floral mutant with increased stigmas, which was isolated from the progenies of the combination of C2/2480. Compared with the wild type rice, the mutant showed dwarf plant, small panicle and grain, curled flag leaf, narrow and opening spikelet, as well as later flowering. Most of florets were made up of normal lodicule, increased stigmas, more pistils, and fewer stamens. Segregation of wild type plants to mutant plants in three F2 population were accorded with the ratio of 3:1, which indicated that the mutant is controlled by a single recessive gene. With SSR (Simple Sequence Repeat) markers screening in a total of 92 F2 mutant individuals derived from the cross of ism/T116, the mutant gene was located between the molecular markers RM3183 and RM3827, at the distances of 2.2 cM and 12.0 cM, respectively, on chromosome 6, and co-segregated with RM11951, RM19953 and RM19961. ISM(t) is a new floral-organ identity gene located on chromosome 6 in rice.This result provided a foundation of map-based cloning and function analysis of ISM(t) gene.

Key words: Rice, Floral organ, Mutant, Genetic analysis, Genetic mapping

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