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作物学报 ›› 2011, Vol. 37 ›› Issue (10): 1771-1778.doi: 10.3724/SP.J.1006.2011.01771

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻基因OsASIE1抗逆功能分析

吴慧敏，黄立钰，潘雅娇，靳鹏，傅彬英*   

  1. 中国农业科学院作物科学研究所 / 农作物基因资源和遗传改良国家重大科学工程, 北京 100081
  • 收稿日期:2011-03-14 修回日期:2011-06-25 出版日期:2011-10-12 网络出版日期:2011-07-28
  • 通讯作者: 傅彬英, E-mail: fuby@caas.net.cn, Tel: 010-82106698
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2008ZX001-003和2009ZX08009-007B)资助。

Function Analysis of the Gene OsASIE1 Responding to Abiotic Stresses in Rice

WU Hui-Min,HUANG Li-Yu,PAN Ya-Jiao,JIN Peng,FU Bin-Ying*   

  1. Institute of Crop Sciences / National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-03-14 Revised:2011-06-25 Published:2011-10-12 Published online:2011-07-28
  • Contact: 傅彬英, E-mail: fuby@caas.net.cn, Tel: 010-82106698

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摘要： 作为重要的植物转录因子家族, AP2/EREBP转录因子在植物发育、激素、病原反应及非生物胁迫如干旱、高盐、低温应答方面起重要作用。本研究发现水稻AP2/EREBP转录因子家族EREBP亚家族成员OsASIE1 (abiotic stress induced EREBP gene)在水稻受到高盐、干旱胁迫时表达量迅速提高, 并且在水稻中超表达OsASIE1能够改善水稻抵抗盐胁迫的能力。凝胶迁移率实验(electrophoresis mobility shift assay, EMSA)表明该转录因子的AP2结构域能够结合干旱应答顺式作用元件DRE (dehydration-responsive element)和乙烯应答元件GCC box (ethylene response element), 推测OsASIE1可能通过结合DRE和GCC box 作用元件调控下游相关基因的表达, 进而调控相关抗逆反应。

关键词: 水稻, AP2/EREBP转录因子, EMSA, 耐盐, 超表达

Abstract: AP2/EREBP transcription factors play an important role in plant development, hormone response, biotic and abiotic stress responses. We identified that OsASIE1, a member of EREBP subfamily of AP2/EREBP transcription factor family in rice, was involved in abiotic stress response. Expression of OsASIE1 wasinduced by drought and salt stresses, and over-expression of OsASIE1 in the transgenic rice plant could improve its tolerance to salt stress.Further electrophoresis mobility shift assay (EMSA) revealed that the AP2 domain of OsASIE1 protein could bind both DRE (dehydration-responsive element) and GCC box (ethylene response element, ERE) in vitro. All these results implicated that OsASIE1 might be involved in abiotic stress response by regulating the expression of downstream genes with DRE and GCC box binding.

Key words: Rice, AP2/EREBP transcription factor, EMSA, Salt tolerance, Over-expression

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