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作物学报 ›› 2012, Vol. 38 ›› Issue (03): 429-435.doi: 10.3724/SP.J.1006.2012.00429

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻短根相关基因OsKSR2的定位

罗丽丽,史俊颖,项显波,丁沃娜*,朱世华   

  1. 宁波大学植物分子生物学研究室，浙江宁波315211
  • 收稿日期:2011-07-11 修回日期:2011-10-15 出版日期:2012-03-12 网络出版日期:2012-01-04
  • 通讯作者: 丁沃娜, E-mail: dwn@zju.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31071388)和宁波市自然科学基金项目(2006A610074)资助。

Mapping of a Short Root-Related Gene OsKSR2 in Rice (Oryza sativa L.)

LUO Li-Li,SHI Jun-Ying,XIANG Xian-Bo,DING Wo-Na*,ZHU Shi-Hua   

  1. Laboratory of Plant Molecular Biology, Ningbo University, Ningbo 315211, China
  • Received:2011-07-11 Revised:2011-10-15 Published:2012-03-12 Published online:2012-01-04
  • Contact: 丁沃娜, E-mail: dwn@zju.edu.cn

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被引次数

4

摘要： 根系是将植物固定于土壤及吸收利用水分和养分的重要器官。本研究从甲基磺酸乙酯(ethyl methane sulfonate，EMS)诱变的籼稻Kasalath突变体库中，筛选到一个水稻根系发育缺陷的突变体，命名为Osksr2 (Oryza sativa kasalath short root 2)，该突变体植株整体矮小，主根、不定根和侧根的伸长都受到抑制。遗传分析表明该突变性状由1对隐性核基因控制，将该基因命名为OsKSR2。将Osksr2纯合体与粳稻日本晴杂交构建F2群体，利用已经公布的水稻SSR标记和自行设计的STS标记进行基因定位，将OsKSR2定位在水稻第8染色体STS标记S27887与S27988之间约101 kb的范围内。通过水稻基因组注释系统共预测到17个开放阅读框(ORF)，没有已知的与根系发育相关的基因。对OsKSR2的定位将为进一步克隆该基因和阐明水稻根系伸长的分子机理奠定基础。

关键词: 水稻, 短根突变体, 基因定位, 遗传分析

Abstract: Theroot system of plant plays an important role in supporting plants and uptaking nutrients and water in soil. In this study, a rice mutant with significantly short roots was isolated from an EMS (ethyl methane sulfonate)-generated mutant library of Kasalath and named as Osksr2(Oryza sativa kasalath short root 2). Osksr2 showed a dwarf phenotype and the elongation of primary roots, adventitious roots and lateral roots in the mutant was severely impaired. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene, and named as OsKSR2. To map OsKSR2, we generated an F2 population by crossing Osksr2 with Nipponbare wild type. By using the published SSR markers and newly designed STS markers, OsKSR2 was mapped to a 101 kb region between the markers S27887 and S27988 on chr.8. Within this region there were seventeen predicted genes, and none of them had been reported to be involved in root development. The study will be helpful for the cloning of OsKSR2 and characterization of the molecular mechanism of root elongation in rice.

Key words: Rice (Oryza sativa L.), Short root mutant, Gene mapping, Genetic analysis

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