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作物学报 ›› 2012, Vol. 38 ›› Issue (03): 563-569.doi: 10.3724/SP.J.1006.2012.00563

• 研究简报 • 上一篇    

油菜角果开裂区结构分化对果实开裂的作用

王婷，暨淑仪，吴鸿*   

  1. 华南农业大学生命科学学院 / 农业生物资源保护与利用国家重点实验室, 广东广州 510642
  • 收稿日期:2011-08-15 修回日期:2011-12-15 出版日期:2012-03-12 网络出版日期:2012-01-04
  • 通讯作者: 吴鸿, E-mail: wh@scau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31070159)资助。

Effect of Structural Differentiation of Fruit Dehiscence Zone on Pod Dehiscence in Oilseed Rape

WANG Ting, JI Shu-Yi,WU Hong*   

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources / College of Life Sciences, South China Agricultural University, Guangzhou 510642, China
  • Received:2011-08-15 Revised:2011-12-15 Published:2012-03-12 Published online:2012-01-04
  • Contact: 吴鸿, E-mail: wh@scau.edu.cn

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1554

被引次数

6

摘要： 运用半薄切片技术、荧光显微镜技术, 观察不同开裂程度的两个油菜品种中双9号和中双11果实发育过程中“果实开裂区”的结构特征、发生发育规律, 探讨两品种油菜果实不同开裂程度的结构差异。结果表明, 油菜果实在发育过程中, 逐渐分化出由胎座框木化细胞、离区细胞、果瓣边缘木化细胞、紧靠果实内果皮的木化层enb细胞共同组成的开裂区; 易开裂的中双9号果实发育进程总体上比不易开裂中双11的早, 前者在15DAF (开花后第15天)时就已经能分辨出离区, 且胎座框细胞、果瓣边缘细胞、enb层细胞的壁都已经木化加厚, 而后者在25DAF才表现出这些特征；中双9号的离区在25DAF完全形成, 而中双11的离区在30DAF完全形成；中双9号离区部分细胞间在30DAF开始出现分离, 而中双11离区部分细胞间在35DAF开始出现分离。另外, 中双9号开裂区胎座框木化细胞间出现较多的裂缝有利于果实的开裂。这些结构和发育特征对于深入研究果实开裂的分子调控和抗角果开裂品种的选育具有理论指导意义。

关键词: 油菜角果, 开裂区, 分化, 果实开裂

Abstract: Oilseed rape plants disperse their seeds by pod-shattering known as Pod dehiscence, which causes yield loss. To investigate the effect of structure differentiation on pod shattering, we studied the developmental process and structural characteristics of fruit “dehiscence zone” of two varieties with different pod shattering behavior Zhongshuang 9 and Zhongshuang 11, by semi-thin section fluorescence microscopy with. Our observations revealed that: (1) rape pod gradually differentiated to form “dehiscence zone” in fruit development, which was composed of dehiscence zone (or separation layer), lignified valve edge, lignified endocarp b and replum cells; (2) The dehiscence zone of Zhongshuang 9 was found at 15DAF (day after flowering), and meanwhile the walls of valve edge cells, endocarp b cells and replum cells were thickened, and that of Zhongshuang 11 did not show these features until 25DAF. The dehiscence zone was fully formed in Zhongshuang 9 at 25DAF, while in Zhongshuang 11 at 30DAF.The separation of dehiscence zone cells was observed at 30DAF in Zhongshuang 9, while this occurred in 35DAF for Zhongshuang 11. In addition, more cell separation sites occurring between lignified replum cells enhanced pod shattering in Zhongshuang 9. These results provide a theoretical guidance for molecular regulation of pod shattering and oilseed rape breeding for pod shattering-resistance.

Key words: Brassica napus pod, Dehiscence zone, Differentiation, Fruit shatter

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