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作物学报 ›› 2012, Vol. 38 ›› Issue (03): 556-562.doi: 10.3724/SP.J.1006.2012.00556

• 研究简报 • 上一篇    下一篇

测定玉米中伏马毒素的免疫亲和层析净化高效液相色谱法

李为喜1，郑床木2，武力1，李欣1，李静梅1，宋敬可1，杨秀兰1，王步军1,*   

  1. LI Wei-Xi1,ZHENG Chuang-Mu2,WU Li1,LI Xin1,LI Jing-Mei1,SONG Jing-Ke1,YANG Xiu-Lan1,WANG Bu-Jun1,*
  • 收稿日期:2011-07-23 修回日期:2011-10-15 出版日期:2012-03-12 网络出版日期:2012-01-04
  • 通讯作者: 王步军, E-mail: wangbj@mail.caas.net.cn, Tel: 010-82105798
  • 基金资助:

    本研究由国家农业行业标准制(修)订项目(2130109)资助。

Determining Fumonisins in Corn by High Performance Liquid Chromatography with Immunoaffinity Column Cleanup

1 中国农业科学院作物科学研究所 / 农业部谷物品质监督检验测试中心，北京100081；2中国农业科学院农业质量标准与检测技术研究所，北京100081   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / Cereal Quality Supervision and Testing Center, Ministry of Agriculture Beijing 100081, China; 2 Institute of Quality Standards & Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2011-07-23 Revised:2011-10-15 Published:2012-03-12 Published online:2012-01-04
  • Contact: 王步军, E-mail: wangbj@mail.caas.net.cn, Tel: 010-82105798

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被引次数

12

摘要： 伏马毒素(Fumonisins)是串珠镰刀菌繁殖产生的一类真菌毒素。玉米在生长和储存过程中极易受到伏马毒素的侵染。流行病学研究结果表明，受到伏马毒素污染的玉米及其制品可导致马白脑软化症、猪肺水肿综合症，还可诱发人类食管癌和胎儿神经管畸形等疾病。本研究建立了应用免疫亲和柱净化高效液相色谱测定玉米中伏马毒素B1和B2的方法，同时，运用统计学方法对该法进行了准确性和再现性评价。结果表明，FB1和FB2线性范围分别为0.06~5.00 μg mL-1和0.04~2.50 μg mL-1，回收率分别为76.6%~93.8%和77.9%~93.4%，FB1和FB2方法定量限分别为0.09 mg kg-1、0.06 mg kg-1，实验室内重复性测定的变异系数均低于5%，实验室间再现性测定的变异系数低于6%。上述结果说明该方法的线性、准确度、精密度、灵敏度及同一实验室重复性和多家实验室的再现性评价结果优良，适合作为伏马毒素的测定方法。应用该方法对310份玉米进行了伏马毒素的测定，结果表明，大田、存储玉米伏马毒素总量范围分别为0.20~9.06 mg kg-1、0.21~6.10 mg kg-1，建议应加强玉米中伏马毒素污染水平监控，保证人畜健康。

关键词: 玉米, 伏马毒素, 高效液相色谱, 免疫亲和柱

Abstract: Fumonisins are mycotoxins produced mainly by Fusarium verticillioides. Corn is frequently found being contaminated by fumonisins during its growth and storage. Epidemic study demonstrated that corn and corn-based products contaminated by fumonisins can cause equine leukoencephalomalacia and swine pulmonary oedema, also induce oesophageal cancer and neural tube defects of mankind. In this paper, a high performance liquid chromatography (HPLC) method with immunoaffinity columncleanup was established to determine the fumonisins B1 (FB1) and B2 (FB2) in corn. Meanwhile, the accuracy and reproducibility of the method were evaluated by statistical technique. The results showed that the linear ranges of FB1 andFB2 were 0.06–5.00 μg mL-1 and 0.04–2.50 μg mL-1, the recovery ranges were 76.6–93.8% and 77.9–93.4%, respectively. Thequantification limit of FB1 andFB2 was 0.09 mg kg-1 and 0.06 mg kg-1, respectively. The coefficient of variation within-laboratory repeatability was below 5%, and that between-laboratories reproducibility was below 6%. Research results indicated that this method possessed good linearity, accuracy, precision, sensitivity, repeatability within a laboratory and among laboratories. Therefore, this method was proved to be suitable for determination of fumonisins in corn. Applying this method, fumonisins in 310 specimens of corn were determined. Results showed that total fumonisins (B1+ B2) ranged from 0.20 to 9.06 mg kg-1 for field corn, and from 0.21 to 6.10 mg kg-1 for stored corn. It is proposed that monitoring fumonisins’ level in corn should be reinforced to protect the health of man and livestock.

Key words: Corn, Fumonisins, HPLC, Immunoaffinity column

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