作物学报 ›› 2015, Vol. 41 ›› Issue (06): 831-837.doi: 10.3724/SP.J.1006.2015.00831
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
谭炎宁1,2,**,孙学武1,**,袁定阳1,孙志忠1,余东1,何强1,段美娟1,*,邓华凤1,2,*,袁隆平1,2,*
TAN Yan-Ning1,2,**,SUN Xue-Wu1,**,YUAN Ding-Yang1,SUN Zhi-Zhong1,YU Dong1,HE Qiang1,DUAN Mei-Juan1,*,DENG Hua-Feng1,2,*,YUAN Long-Ping1,2,*
摘要:
转绿型叶色突变体是研究植物叶绿体分化与发育的基础材料。grc2是利用60Co-γ射线诱变籼型三系保持系T98B后获得的单叶独立转绿型黄化突变体。grc2植株上任一叶片刚抽出时为黄色,在生长10 d左右后变绿,具有单叶不依赖于植株特定发育阶段而独立转绿的特性。与野生型T98B相比,grc2黄化叶片的总叶绿素和叶绿素b含量显著降低,叶绿体滞留在黄化质体阶段,表明grc2可能在叶片早期发育中起关键作用。遗传分析表明,grc2受一对隐性核基因独立控制;利用源于grc2/Nipponbare的F2群体的960个突变单株,将grc2基因定位在STS标记S254与S258之间约31 kb的范围内,该区域含有5个未报道过的注释基因。这些结果为grc2的克隆及功能研究提供了重要信息。
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