利用棉花海陆种间染色体片段导入系剖析光合色素含量的遗传基础

doi:10.3724/SP.J.1006.2012.00947

摘要/Abstract

摘要： 光合作用是棉花产量和品质的基础, 而光合色素在光能的吸收、传递和转换中起着重要作用。利用海陆棉种间连续回交和标记辅助选择培育的染色体片段导入系群体, 对棉花叶片中光合色素含量进行了QTL定位研究。通过软件QTLIciMapping 3.0, 检测到LOD>3.0的影响叶绿素a含量、叶绿素b含量、类胡萝卜素含量、叶绿素a/b值和叶绿素总含量等5个性状的44个QTL, 其中15个在2年中都被检测到。44个QTL主要分布在A1(chr.1)、A8(chr.8)、A9(chr.9)、A11(chr.11)、A13(chr.13)、D1(chr.15)、D3(chr.17)、D5(chr.19)、D6(chr.25)、D7(chr.16)、D8(chr.24)、D9(chr.23)、D10(chr.20)、D11(chr.21)和D12(chr.26)等15条染色体上, 可解释1.25%~5.59%的表型变异。发现SSR标记NAU3714(chr.D1)的染色体区段上存在提高叶绿素a和b含量、叶绿素总含量和类胡萝卜素含量等4性状的QTL, 结合修饰回交育种技术开展棉花的高光效育种可能带来棉花产量育种上的突破。

关键词: 染色体片段导入系, 海岛棉, 光合色素含量, QTL

Abstract: Photosynthesis provides an important foundation for cotton yield and fiber quality. Photosynthetic pigments play an important role in absorption, transfer, and transition of photo energy. In this study, quantitative trait loci (QTLs) mapping was conducted for leaf photosynthetic pigment content using interspecific chromosome segment introgression lines (CSIL). Forty four QTLs (LOD >3) for chlorophyll-a content, chlorophyll-b content, carotenoid content, chlorophyll-a/b ratio value and total chlorophyll content were detected in CSIL population by QTLIciMapping 3.0. Among them, fifteen QTLs were detected in two years. Forty four QTLs were located on fifteen chromosomes including A1(chr.1), A8(chr.8), A9(chr.9), A11(chr.11), A13(chr.13), D1(chr.15), D3(chr.17), D5(chr.19), D6(chr.25), D7(chr.16), D8(chr.24), D9(chr.23), D10(chr.20), D11(chr.21), and D12(chr.26) with the explained phenotypic variation of 1.25%–5.59%. QTLs (qCa-D1-1, qCb-D1-1, qCx.c-D1-1, and qTC-D1-1) influencing chlorophyll-a content, chlorophyll-b content, carotenoid content and total chlorophyll content were located near NAU3714 on chr.D1. It may make a breakthrough in increasing cotton yield through the breeding for high photosynthetic efficiency using the chromosome segment near NAU3714 with modified backcrossing pyramiding breeding (MBPB) technique.

Key words: Chromosome segment introgression lines, Gossypium barbadense, Photosynthetic pigment content, QTL

王鹏,张天真. 利用棉花海陆种间染色体片段导入系剖析光合色素含量的遗传基础[J]. 作物学报, 2012, 38(06): 947-953.

WANG Peng,ZHANG Tian-Zhen. Genetic Dissection of Photosynthetic Pigment Content in Cotton Interspecific Chromosome Segment Introgression Lines[J]. Acta Agron Sin, 2012, 38(06): 947-953.

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