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作物学报 ›› 2012, Vol. 38 ›› Issue (09): 1710-1715.doi: 10.3724/SP.J.1006.2012.01710

• 耕作栽培·生理生化 • 上一篇    下一篇

NO对干旱条件下小麦幼苗PSII功能特性的调节效应

邵瑞鑫1,2，信龙飞1，杨青华1，上官周平2,*   

  1. 1 河南农业大学农学院 / 河南省粮食作物生理生态与遗传改良重点实验室，河南郑州 450002; 2 黄土高原土壤侵蚀与旱地农业国家重点实验室，陕西杨凌 712100
  • 收稿日期:2011-12-21 修回日期:2012-04-20 出版日期:2012-09-12 网络出版日期:2012-07-03
  • 通讯作者: 上官周平, E-mail: shangguan@ms.iswc.ac.cn
  • 基金资助:

    本研究由中国科学院知识创新工程重大项目课题(KZCX2-YW-JC408)和河南省基础研究项目(122300410012)资助。

Modulation of Exogenous Nitric Oxide on Photosystem II Functions in Wheat Seedlings under Drought Stress

SHAO Rui-Xin1,2,XIN Long-Fei1,YANG Qing-Hua1,SHANG-GUAN Zhou-Ping2,*   

  1. 1 Agronomy College of Henan Agriculture University / Key Laboratory of Physiology, Ecology and Genetic Improvement of Food Crops in Henan Province, Zhengzhou 450002, China; 2 State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Yangling, 712100, China
  • Received:2011-12-21 Revised:2012-04-20 Published:2012-09-12 Published online:2012-07-03
  • Contact: 上官周平, E-mail: shangguan@ms.iswc.ac.cn

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被引次数

6

摘要： 以0.1 mmol L-1 SNP为NO供体，对小麦幼苗根系进行-0.5 MPa PEG胁迫处理，研究了NO对胁迫后叶片电子传递、光能分配和反应中心开放等PSII功能的影响，以探讨NO在干旱条件下对植物光合作用的调节作用。在干旱胁迫的第1天和第3天，SNP处理不但增加了水势(Ψw)和叶绿素含量，且能维持PSII反应中心的电子传递(ФPSII和Fm/Fo)及潜在高光合效率(Fv/Fo)；干旱胁迫减少了PSII反应中心开放的比例(qP)和PSII反应中心捕获光能的转化效率，但SNP处理后PSII开放反应中心的比例增加，利于干旱条件下叶片吸收的能量用于光化学反应(Pr)和PSII反应中心安全地耗散过剩光能。综上所述，干旱条件下NO对小麦幼苗叶片的PSII功能具有调节作用。

关键词: 一氧化氮, PSII功能, 叶绿素, 小麦, 干旱胁迫

Abstract: Nitric oxide (NO) has emerged as a key molecule involving in tolerating various abiotic stresses that induce physiological responses in plants. To characterize the role of NO in tolerance of photosystem II (PSII) to drought stress induced by polyethylene glycol (PEG), we used sodium nitroprusside (SNP) of 0.1 mmol L-1as exogenous NO donor to study the biologically protective effects of NO at a low concentration on PSII functions in wheat (Triticum aestivum L.) seedlings hydroponically grown under -0.5 MPa PEG stress. Under drought stress, SNP supplement increased the water potential (Ψw) and chlorophyll content, maintained favorable status of electron transport (ФPSII and Fm/Fo) and potential high activity of PSII reaction centers (Fv/Fo) on the first and third days of treatment. The open proportion of PSII reaction centers (qP) and absorbed-light allocation to photochemical reaction(Pr)were higher in the treatment of SNP supplement under drought stress than in only PEG treatment. SNP alleviated drought-induced inhibition to absorbed-energy conversion of open PSII (Fv′/Fm′), safely dissipating excessive light energy in PSII reaction centers. Therefore, NO could modulate the PSII functions in photosynthesis of wheatseedlings hydroponically exposed to PEG stress.

Key words: Nitric oxide, PSII functions, Chlorophyll content, Wheat, Drought stress

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