欢迎访问作物学报,今天是

作物学报 ›› 2012, Vol. 38 ›› Issue (12): 2206-2216.doi: 10.3724/SP.J.1006.2012.02206

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用与大豆灰斑病抗性基因连锁的SSR标记构建品种(系)的分子身份证

丁俊杰1,姜翠兰2,顾鑫1,杨晓贺1,赵海红1,申宏波1,仕相林4,刘春燕3,胡国华3,*,陈庆山4,*   

  1. 1黑龙江省农业科学院佳木斯分院 / 农业部佳木斯作物有害生物科学观测实验站,黑龙江佳木斯 154007;2 黑龙江农垦科学院作物研究所,黑龙江佳木斯 154007;3 黑龙江农垦科研育种中心,黑龙江哈尔滨 150030;4 东北农业大学农学院,黑龙江哈尔滨 150030
  • 收稿日期:2012-02-22 修回日期:2012-08-05 出版日期:2012-12-12 网络出版日期:2012-10-08
  • 通讯作者: 胡国华, E-mail: hugh757@vip.163.com; 陈庆山, E-mail: qshchen@sohu.com
  • 基金资助:

    本研究由黑龙江省自然科学基金项目(C200936), 国家公益性行业(农业)科研专项(201103016-03A2)和国家现代农业产业技术体系建设佳木斯综合试验站项目资助。

Establishment of Molecular ID of Soybean Varieties (Lines) with SSR Marker Linked to Resistance Genes of Cercospora sojina

DING Jun-Jie1,JIANG Cui-Lan2,GU Xin1,YANG Xiao-He1,ZHAO Hai-Hong1,SHEN Hong-Bo1,SHI Xiang-Lin3,LIU Chun-Yan3,HU Guo-Hua3,*,CHEN Qing-Shan4,*   

  1. 1 Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences / Observation and Experiment Station of Crop Pests of Jiamusi, Ministry of Agriculture, Jiamusi 154007, China; 2 Institute of Crop, Heilongjiang Academy of Land Reclamation Science, Jiamusi 154007, China; 3 Land Reclamation Research & Breeding Centre of Heilongjiang, Harbin 150090, China; 4 College of Agriculture, Northeast Agricultural University, Harbin 150030, China
  • Received:2012-02-22 Revised:2012-08-05 Published:2012-12-12 Published online:2012-10-08
  • Contact: 胡国华, E-mail: hugh757@vip.163.com; 陈庆山, E-mail: qshchen@sohu.com

摘要:

以黑龙江省29个大豆育种单位的103份已鉴定大豆灰斑病3个生理小种抗性的大豆品种()为材料,选择与大豆灰斑病抗病基因连锁的19SSR标记检测,获得等位变异数86个,每个标记检测到的等位变异数分布在2~6个之间,平均为4.42个。应用遗传统计软件(genetics statistics 3.0)分析表明, 标记的多样性指数介于0.198~0.751之间,平均多样性指数为0.606。品种()特异指数差异较大,介于46.592~481.541之间,平均为87.415。根据标记的等位基因数,使用ID Analysis 1.0软件分析表明,利用与大豆抗灰斑病基因连锁的7SSR标记(Satt565Satt547Satt431Sct_186SOYGPATRSatt244Sat_151)就能有效区分各品种(),因此利用这7个标记构建了供试品种()的分子身份证。

关键词: 大豆灰斑病, 生理小种, SSR, 分子身份证

Abstract:

One hundred and three soybean varieties (lines) developed by 29 breeding units from six accumulated temperature zones in Heilongjiang were investigated. The resistances of 103 soybean varieties (lines) were identified with three races of Cercospora sonija. Eighty-six polymorphic alleles were detected with 19 SSR markers, for each primer, two to six allele variations were detected in all varieties (lines), with an average of 4.42. The diversity index of the 19 SSR loci ranged from 0.198 to 0.751 with an average of 0.606. The results of calculating specific index and genetic similarity among varieties (lines) showed that specific index of varieties (lines) ranged from 46.592 to 481.541, and the average was 87.415. According to fragment size of allele variation, the data calculated from the PAGE bands were analyzed by the software ID Analysis 1.0. Seven markers (Satt565, Satt547, Satt431, Sct_186, SOYGPATR, Satt244, and Sat_151) linked to the gene controlling resistance to Cercospora sojina were used to identify all 103 soybean lines, so that a set of molecular ID of soybean varieties (lines) was established.

Key words: Soybean frogeye leaf spot, Physiological race, SSR, Molecular ID

[1]Grassi F, Labra M, Imazio S, Spada A, Sgorbati S. Evidence of a secondary grapevine domestication centre detected by SSR analysis. Theor Appl Genet, 2003, 107: 1315−1320



[2]Matus I A, Hayes P M. Genetic diversity in three groups of barley germplasm assessed by simple sequence repeats. Genome, 2002, 45: 1095−1106



[3]Anthony F, Combes M, Astorga C, Bertrand B, Graziosi G. The origin of cultivated Coffea arabica L. varieties revealed by AFLP and SSR markers. Theor Appl Genet, 2002, 104: 894−900



[4]Wu W(武文), Deng Q-Y(邓启云), Zhou L-J(周丽洁), Zhu X-Q(朱校奇), Chen C-G(陈春光), Zhuang W(庄文), Jin D-M(金德敏), Wang B(王斌). Establishment of DNA fingerprinting with SSR for Y58S and some other elite parents of two-line hybrid rice. Hybrid Rice (杂交水稻), 2008, 23(3): 52−56 (in Chinese with English abstract)



[5]Wang L-M(王黎明), Jiao S-J(焦少杰), Jiang Y-X(姜艳喜), Yan H-D(严洪冬), Su D-F(苏德峰), Sun G-Q(孙广全). Establishment of molecular identity in 142 sweet sorghum varieties. Acta Agron Sin (作物学报), 2011, 37(11): 1975−1983 (in Chinese with English abstract)



[6]Li X-H(李晓辉), Li X-H(李新海), Gao W-W(高文伟), Tian Q-Z(田清震), Li M-S(李明顺), Ma F-M(马凤鸣), Zhang S-H(张世煌). Establishment of DNA fingerprinting database of maize hybrids and its application in parentage identification. Acta Agron Sin (作物学报), 2005, 31(3): 386−391 (in Chinese with English abstract)



[7]Wang F-G(王凤格), Zhao J-R(赵久然), Guo J-L(郭景伦), Liu L-Z(刘龙洲). Series of research on establishing DNA finger- printing poll of Chinese new maize cultivars: I. The establishment of a standard SSR system fitting for maize cultivars identification. Maize Sci (玉米科学), 2003, 11(1): 3−6 (in Chinese with English abstract)



[8]Liu P-W(刘平武), Zhou G-L(周国岭), Yang G-S(杨光圣), Fu T-D(傅廷栋). Fingerprints construction of hybrid parents in Brassica napus and its utilization in hybrid purity test. Acta Agron Sin (作物学报), 2005, 31(5): 640−646 (in Chinese with English abstract)



[9]Qi L(齐兰), Wang W-Q(王文泉), Zhang Z-W(张振文), Ye J-Q(叶剑秋), Li K-M(李开绵). DNA fingerprinting analysis of 18 cassava varieties using sequence-related amplified polymorphism markers. Acta Agron Sin (作物学报), 2010, 36(10): 1642−1648 (in Chinese with English abstract)



[10]Fu Y-H(付瑜华), Li J(李杰), Wang H-Y(王海燕), Yang Z-X(杨子贤), Wang W-Q(王文泉). Establishment of fingerprints for several commercial cultivars in cassava. Plant Genet Resour (植物遗传资源学报), 2007, 8(1): 51−55 (in Chinese with English abstract)



[11]Piperidis G, Rattey A R, Taylor G O, Cox M C. DNA markers: a tool for identifying sugarcane varieties. Aust Sugarcane, 2004, 8(suppl): 1−8



[12]Wang L-X(王立新), Li Y-F(李云伏), Chang L-F(常利芳), Huang L(黄岚), Li H-B(李宏博), Ge L-L(葛玲玲), Liu L-H(刘丽华), Yao J(姚骥), Zhao C-P(赵昌平). Method of ID constitution for wheat cultivars. Acta Agron Sin (作物学报), 2007, 33(10): 1738−1740 (in Chinese with English abstract)



[13]Zheng H-Y(郑海燕), Su J-G(粟建光), Dai Z-G(戴志刚), Li Y(李燕), Chen J-Q(陈基权), Gong Y-C(龚友才). Establishment of molecular identity for kenaf germplasm using ISSR and RAPD markers. Sci Agric Sin (中国农业科学), 2010, 43(17): 3499−3510 (in Chinese with English abstract)



[14]Gao Y-L(高运来), Zhu R-S(朱荣胜), Liu C-Y(刘春燕), Li W-F(李文福), Jiang H-W(蒋洪蔚), Li C-D(李灿东), Yao B-C(姚丙晨), Hu G-H(胡国华), Chen Q-S(陈庆山). Establishment of molecular ID in soybean varieties in Heilongjiang, China. Acta Agron Sin (作物学报), 2009, 35(2): 211−218 (in Chinese with English abstract)



[15]Li H-Y(李英慧). Genetic structure and diversity for chinese soybean (Glycine max L.) landraces in China and SNAPs marker development at rhg1 locus underlying resistance to soybean Cyst nematode. PhD Dissertation of Chinese Academy of Agricultural Sciences, 2005. pp 1−82 (in Chinese with English abstract)



[16]Qin J(秦君), Chen W-Y(陈维元), Guan R-X(关荣霞), Jiang C-X(姜成喜), Li Y-H(李英慧), Fu Y-S(付亚书), Liu Z-X(刘章雄), Zhang M-C(张孟臣), Chang R-Z(常汝镇), Qiu L-J(邱丽娟). SSR analysis of genetic basis of Chinese soybean varieties broaded by oversea germplasm. Chin Sci Bull (科学通报), 2006, 51(6): 686−692 (in Chinese)



[17]Hu G-H(胡根海), Hu H-Y(胡海燕). Genetic diversity analysis of high oil soybean based on SSR markers. Acta Agric Univ Jiangxiensis (江西农业大学学报), 2008, 30(4): 638−642 (in Chinese with English abstract)



[18]Mian M A R, Missaoui A M, Walker D R. Phillips D V, Boerma H R. Frogeye leaf spot of soybean: A review and proposed race designations for isolates of Cercospora sojina Hara. Crop Sci, 2008, 48: 14−24



[19]Bachman M S, Tamulonis J P, Nickell C D. Bent A F. Molecular markers linked to brown stem rot resistance genes Rbs1 and Rbs2 in soybean. Crop Sci, 2001, 41: 527−535



[20]Akem C N, Kashiell K E. Effect of planting date on severity of frogeye leaf spot and grain yield of soybeans. Crop Protect, 1994, 13: 607−610



[21]Jiang C-L(姜翠兰), Ding J-J(丁俊杰), Wen J-Z(文景芝), Hu G-H(胡国华), Chen Q-S(陈庆山), Liu C-Y(刘春燕). Identification and mapping of the Cercospora sojina race 15 resistance gene in soybean. Acta Phytophyl Sin (植物保护学报), 2011, 38(2): 116−120 (in Chinese with English abstract)



[22]Hu G-H(胡国华), Yu F-Y(于凤瑶), Sun W-B(孙文本), Luan H-H(栾怀海), Zhou S-Q(周顺启). The biotic genetic analysis for soybean resistance to frogeye spot. Soybean Sci (大豆科学), 1996, 15(1): 30−33 ( in Chinese with English abstract)



[23]Yang Q-K(杨庆凯), Qi Y-A(齐永安), Wu T-L(武天龙), Zhang X-G(张晓刚). Resistant test and a study on inheritance of resistance of soybean frogeye leaf spot. J Northeast Agric Univ (东北农业大学学报), 1988, 19(1): 1−14( in Chinese with English abstract)



[24]Dong W(董伟), Chen S-Y(陈受宜), Zou J-J(邹继军), Yang Q-K(杨庆凯), Cao Y-P(曹越平). Genetic analysis of resistance to race 7 of Cercospora sojina and identification of RAPD marker of resistance gene. Sci Bull (科学通报), 43(21): 2302−2307 (in Chinese)



[25]Dong W(董伟), Yang Q-K(杨庆凯), Sen Y-G(沈义国). Identification of RAPD marker of resistance gene to race 1 of Cercospora sojina. High Tech Commun (高科技通讯), 1999(10): 48−51 (in Chinese)



[26]Zhang W-H(张文慧), Chen Q-S(陈庆山), Yang Q-K(杨庆凯), Li W-B(李文滨), Wang W-H(王文辉), Liu C-Y(刘春燕), Chen L-J(陈立君), Liu H-Y(刘海燕), Shan J-X(单继勋). Analysis of resistant gene against Cercospora sojina race 1 in soybean with SSR marker. Soybean Sci (大豆科学), 2004, 23(3): 169−173 (in Chinese with English abstract)



[27]Mian M A R, Wang T Y, Phillips D V, Alvernaz J, Boerma H R. Molecular mapping of the Rcs3 gene for resistance to frogeye leaf spot in soybean. Corp Sci, 1999, 39(6): 1687−1691



[28]Chen Q-S(陈庆山), Zhang Z-C(张忠臣), Liu C-Y(刘春燕), Xin D-W(辛大伟), Shan D-P(单大鹏), Qiu H-M(邱红梅), Shan C-Y(单彩云). QTL analysis of major agronomic traits in soybean. Sci Agric Sin (中国农业科学), 2007, 40(1): 41−47 (in Chinese with English abstract)



[29]Chen Q-S(陈庆山). Comparative analysis of specialty index and genetic similarity on soybean germplasm with resistance to Cercospora sojina Hara. China Biotechnol (中国生物工程杂志), 2005, 25(suppl): 155−158 (in Chinese with English abstract)



[30]Wang J-Y(王静毅), Chen Y-Y(陈业渊), Huang B-Z(黄秉智), Yu F(于飞), Wu Y-T(武耀廷). Establishment of fingerprinting for bananas (Musa nana) by SSR marker. J Fruit Sci, 2009, 26(5): 733−738 (in Chinese)



[31]Russell J, Fuller J, Young G, Thomas B, Taramino G, Macaulay M, Waugh R, Powell W. Discrimination between barley genotypes using microsatelite markers. Genome, 1997, 40: 442−450



[32]Akagi H, Yokozeki Y, Inagaki A, Fujimura T. Highly polymorphic microsatellites of rice consist of AT repeats and a classification of closely related cultivars with these microsatellite loci. Theor Appl Genet, 1997, 94: 61−67

[1] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证[J]. 作物学报, 2022, 48(4): 908-919.
[2] 张霞, 于卓, 金兴红, 于肖夏, 李景伟, 李佳奇. 马铃薯SSR引物的开发、特征分析及在彩色马铃薯材料中的扩增研究[J]. 作物学报, 2022, 48(4): 920-929.
[3] 王琰琰, 王俊, 刘国祥, 钟秋, 张华述, 骆铮珍, 陈志华, 戴培刚, 佟英, 李媛, 蒋勋, 张兴伟, 杨爱国. 基于SSR标记的雪茄烟种质资源指纹图谱库的构建及遗传多样性分析[J]. 作物学报, 2021, 47(7): 1259-1274.
[4] 韩贝, 王旭文, 李保奇, 余渝, 田琴, 杨细燕. 陆地棉种质资源抗旱性状的关联分析[J]. 作物学报, 2021, 47(3): 438-450.
[5] 练云, 王金社, 魏荷, 李金英, 弓贵明, 王树峰, 张晶鹏, 李茂林, 郭建秋, 卢为国. 山西省古交市大豆胞囊线虫新小种X12分布调查[J]. 作物学报, 2021, 47(2): 237-244.
[6] 刘少荣, 杨扬, 田红丽, 易红梅, 王璐, 康定明, 范亚明, 任洁, 江彬, 葛建镕, 成广雷, 王凤格. 基于农艺及品质性状与SSR标记的青贮玉米品种遗传多样性分析[J]. 作物学报, 2021, 47(12): 2362-2370.
[7] 郭艳春, 张力岚, 陈思远, 祁建民, 方平平, 陶爱芬, 张列梅, 张立武. 黄麻应用核心种质的DNA分子身份证构建[J]. 作物学报, 2021, 47(1): 80-93.
[8] 王恒波,祁舒婷,陈姝琦,郭晋隆,阙友雄. 甘蔗栽培种单倍体基因组SSR位点的发掘与应用[J]. 作物学报, 2020, 46(4): 631-642.
[9] 张红岩,杨涛,刘荣,晋芳,张力科,于海天,胡锦国,杨峰,王栋,何玉华,宗绪晓. 利用EST-SSR标记评价羽扇豆属(Lupinus L.)遗传多样性[J]. 作物学报, 2020, 46(3): 330-340.
[10] 张力岚, 张列梅, 牛焕颖, 徐益, 李玉, 祁建民, 陶爱芬, 方平平, 张立武. 黄麻SSR标记与纤维产量性状的相关性[J]. 作物学报, 2020, 46(12): 1905-1913.
[11] 刘荣, 王芳, 方俐, 杨涛, 张红岩, 黄宇宁, 王栋, 季一山, 徐东旭, 李冠, 郭瑞军, 宗绪晓. 利用2个F2群体整合中国豌豆高密度SSR遗传连锁图谱[J]. 作物学报, 2020, 46(10): 1496-1506.
[12] 叶卫军,陈圣男,杨勇,张丽亚,田东丰,张磊,周斌. 绿豆SSR标记的开发及遗传多样性分析[J]. 作物学报, 2019, 45(8): 1176-1188.
[13] 陈芳,乔麟轶,李锐,刘成,李欣,郭慧娟,张树伟,常利芳,李东方,阎晓涛,任永康,张晓军,畅志坚. 小麦新种质CH1357抗白粉病遗传分析及染色体定位[J]. 作物学报, 2019, 45(10): 1503-1510.
[14] 薛延桃,陆平,史梦莎,孙昊月,刘敏轩,王瑞云. 新疆、甘肃黍稷资源的遗传多样性与群体遗传结构研究[J]. 作物学报, 2019, 45(10): 1511-1521.
[15] 姚嘉瑜,张立武,赵捷,徐益,祁建民,张列梅. 黄麻全基因组SSR鉴定与特征分析[J]. 作物学报, 2019, 45(1): 10-17.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!