作物学报 ›› 2013, Vol. 39 ›› Issue (04): 609-616.doi: 10.3724/SP.J.1006.2013.00609
牛远,谢芳腾,布素红,谢尚潜,韩世凤,耿青春,刘兵,章元明*
NIU Yuan,XIE Fang-Teng,BU Shu-Hong,XIE Shang-Qian,HAN Shi-Feng,GENG Qing-Chun,LIU Bing,ZHANG Yuan-Ming*
摘要:
在溧水中子黄豆×南农493-1衍生的504个F2:6家系中选择Satt331~Satt592目标区间7个杂合单株和168个重组单株,衍生成356株RHL-F2个体(群体I)和168个重组体家系(群体II)。群体II来自142个F2:6家系,若每个F2:6家系只保留1个重组家系则构成群体III。采用lasso和复合区间作图(CIM)法检测3个群体粒形性状2种指标的QTL。结果表明, lasso法检测到的粒长关联标记是O19和S21/Satt331,而CIM检测到的QTL区间是S21~S22和O23~O19;lasso法检测到的粒宽关联标记是O19/O21,而CIM检测到的QTL区间是O23~O19/O19~O21;长宽比与S21~S22关联是由于粒长QTL引起的,与O23~O19 /O19~O21关联是由于粒长和粒宽QTL引起的。将原Satt331~Satt592目标区间的粒长QTL剖分为与标记S21~S22和O23~O19/O19~O21关联的2个多效性QTL。根据大豆基因注释数据库,Glyma10g35240和Glyma10g34980可能是控制粒形性状发育的候选基因。
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