作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1039-1044.doi: 10.3724/SP.J.1006.2013.01039
郭爽**,李云峰**,任德勇,张天泉,何光华*
GUO Shuang**, LI Yun-Feng**, REN De-Yong, ZHANG Tian-Quan, and HE Guang-Hua*
摘要:
鉴定和克隆水稻花器官突变体新基因,对了解水稻花器官发育的分子遗传机制和分子信号调控途径有着重要的作用。本研究报道了1个水稻颖壳异常突变体,来源于EMS (ethyl methane sulfonate)处理的缙恢10号(Oryza sativa)诱变群体,暂被命名为degraded hull 2 (dh2)。表型分析发现突变体小花第一轮内稃或外稃横向细胞数目减少,导致内稃或外稃变窄而不能正常勾合,从而呈现开裂现象,其内三轮花器官均无明显变化。遗传分析表明该突变性状受1个隐性单基因控制。利用群体分离分析法(bulked segregation analysis, BSA),将DH2基因定位在第3染色体的IND-5和IND-14之间,遗传距离分别为0.99 cM和1.49 cM。该研究结果为DH2基因的图位克隆奠定了基础,对水稻花发育生物学研究具有重要的意义。
| [1]Bowman J L, Smyth D R, Meyerowitz E M. Genetic interactionsamong floral homeotic genes of Arabidopsis. Development, 1991, 112: 1–20[2]Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353: 31–37[3]Ditta G, Pinyopich A, Robles P, Pelaz S, Yanofsky M F. The SEP4 gene of Arabidopsis thaliana functions in floral organ and meristem identity. Curr Biol, 2004, 14: 1935–1940[4]Theissen G, Saedler H. Plant biology: Floral quartets. Nature, 2001, 409: 469–471[5]Weigel D, Meyerowitz E M. The ABCs of floral homeotic genes. Cell, 1994, 78: 203–209[6]Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y. SUPERWOMAN1, DROOPING LEAF genes control floral organ identity in rice. Development, 2003, 130: 705–718[7]Whipple C J, Ciceri P, Padilla C M, Ambrose B A, Bandong S L, Schmidt R J. Conservation of B-class floral homeotic gene function between maize and Arabidopsis. Development, 2004, 131: 6083–6091[8]Yamaguchi T, Lee D Y, Miyao A, Hirochika H, An G H, Hirano H Y. Functional diversification of the two C-class MADS-box genes OSMADS3 and OSMADS58 in Oryza sativa. Plant Cell, 2006, 18: 15–28[9]Mandel M A, Brown C G, Savidge B, Yanofsky M F. Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature, 1992, 360: 273–277[10]Drews G N, Bowman J L, Meyerowitz E M. Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product. Cell, 1991, 65: 991–1002[11]Mizukami Y, Ma H. Ectopic expression of the floral homeotic gene agamous in transgenic Arabidopsis plants alters floral organ identity. Cell, 1992, 71: 119–131[12]Xiao H, Tang J F, Li Y F, Wang W M, Li X B, Jin L, Xie R, Luo H F, Zhao X F, Meng Z, He G H, Zhu L H. STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice. Plant J, 2009, 59: 789–801[13]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832[14]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325[15]Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (遗传), 2003, 25(6): 705–707 (in Chinese with English abstract)[16]Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene RL10 (t) in rice (Oryza sativa L.). Genome, 2007, 50: 811–817[17]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174–181[18]Kosambi D D. The estimation of map distances from recombination values. Ann Eugen, 1944, 12: 172–175[19]Prasad K, Parameswaran S, Vijayraghavan U. OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs, Plant J, 2005, 43: 915–928[20]Jin Y, Luo Q, Tong H N, Wang A J, Cheng Z J, Tang J F, Li D Y, Zhao X F, Li X B, Wan J M, Jiao Y L, Chu C C, Zhu L H. An at-hook gene is required for palea formation and floral organ number control in rice. Dev Biol, 2011, 359: 277–288[21]Agrawal K G, Abe K, Yamazaki M, Miyao A, Hirochika A. Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss of function mutants of the OsMADS1 gene. Plant Mol Biol, 2005, 59: 125–135[22]Chen Z X, Wu J G, Ding W N, Chen H M, Wu P, Shi C H. Morphogenesis and molecular basis on naked seed rice, a novel homeotic mutation of OsMADS1 regulating transcript level of AP3 homologue in rice. Planta, 2006, 223: 882–890[23]Wang K J, Tang D, Hong L L, Xu W Y, Huang J, Li M, Gu M H, Xue Y B, Cheng Z K. DEP and AFO regulate reproductive habit in rice. PloS Genet, 2010, 6: e1000818[24]Yuan Z, Gao S, Xue D W, Luo D, Li L T, Ding S Y, Yao X, Wilson Z A, Qian Q, Zhang D B. RETARDED PALEA1 controls palea development and floral zygomorphy in rice. Plant Physiol, 2009, 149: 235–244[25]Shinnosuke O, Mayumi K, Maiko S, Akio M, Hirohiko H, Eiji U, Yasuo N, Hitoshi Y. MOSAIC FLORAL ORGANS 1, an AGL6-like MADS box gene, regulates floral organ identity and meristem fate in rice. Plant Cell, 2009, 21: 3008–3025[26]Sang X C, Li Y F, Luo Z K, Ren D Y, Fang L K, Wang N, Zhao F M, Ling Y H, Yang Z L, Liu Y S, He G H. CHIMERIC FLORAL ORGANS 1, encoding a Monocot-specific MADS-box protein, regulates floral organ identity in rice. Plant Physiol, 2012, 160: 788–807[27]Zhang Y-Z(张玉烛), Zhang G-H(张桂和), Zhu G-C(朱国才), Deng Q-Y(邓启云), Zhan Q-C(詹庆才). Effects of overcast and raining on flowering, fertilizing and seed setting of early rice. Chin J Rice Sci (中国水稻科学), 1995, 9(3): 173–178 (in Chinese with English abstract)[28]Wang Z(王忠), Gu Y-J(顾蕴洁), Yu H-L(于洪亮), Shi H-Y(石火英), Gao Y-Z(高煜珠). Studies on the cause of formation of deformed kernel of wild abortion type male sterile line in rice. Sci Agric Sin (中国农业科学), 1995, 28(6): 25–31 (in Chinese with English abstract) |
| [1] | 胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912. |
| [2] | 邹仪妹, 徐敏, 汪海洋, 姚辉, 王加峰, 刘浩, 任代胜. 两系不育系水稻幼苗根系响应盐胁迫的转录因子调控网络鉴定[J]. 作物学报, 2026, 52(6): 1728-1742. |
| [3] | 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372. |
| [4] | 陈伟, 卫万娟, 赵其兵, 常东伟, 余凌波, 翟鹏飞, 冯志明, 陈宗祥, 任仰涛, 杨鹏, 刘海浪, 李珍富, 杨永乐, 金彦刚, 左示敏. 利用CRISPR/Cas9编辑Hd6基因创制优质早熟水稻新种质[J]. 作物学报, 2026, 52(4): 1046-1056. |
| [5] | 石少阶, 刘凯, 陈姿夷, 王卉颖, 李三和, 周雷, 游艾青. 水稻矮化多分蘖基因DMT1的克隆与功能分析[J]. 作物学报, 2026, 52(4): 1022-1034. |
| [6] | 覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812. |
| [7] | 叶凡, 李帅, 李思宇, 陈云, 窦超银, 刘立军. 不同节水灌溉方式对东北稻区水稻产量和群体质量的影响[J]. 作物学报, 2026, 52(3): 895-907. |
| [8] | 王婵, 吴莹莹, 李文奇, 李霞, 王芳权, 周彤, 杨杰. 基于HRM技术开发水稻抗条纹叶枯病基因STV11功能标记[J]. 作物学报, 2025, 51(9): 2547-2556. |
| [9] | 陈惠莹, 何嘉欣, 朱斌, 黄士轩, 周星佑, 伍君权, 杨美艳. 水稻黄单胞菌噬菌体vB_XaS_HDB2的全基因组分析和生物学特性研究[J]. 作物学报, 2025, 51(8): 2087-2099. |
| [10] | 杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724. |
| [11] | 雷松翰, 范骏扬, 车艳奕, 代永东, 郑雨萌, 田维江, 桑贤春, 王晓雯. 水稻内卷叶突变体acl3的鉴定及调控基因的功能分析[J]. 作物学报, 2025, 51(6): 1467-1479. |
| [12] | 李福媛, 杨奕, 马继琼, 许明辉, 林良斌, 孙一丁. 水稻OsPUB4基因克隆、激素诱导表达分析与互作蛋白筛选[J]. 作物学报, 2025, 51(6): 1690-1700. |
| [13] | 李世鹏, 陈才武, 张晶, 吕恬, 傅廷栋, 易斌. 基于改进U-Net++模型的油菜pol TCMS温敏两系育性等级鉴定及温度育性关系的量化研究[J]. 作物学报, 2025, 51(6): 1423-1434. |
| [14] | 杨思杰, 杜启迪, 柴守玺, 熊宏春, 谢永盾, 赵林姝, 古佳玉, 郭会君, 刘录祥. 小麦小旗叶突变性状基因定位与遗传分析[J]. 作物学报, 2025, 51(6): 1548-1557. |
| [15] | 袁鑫, 赵卓凡, 赵瑞清, 刘孝伟, 郑名敏, 刘育生, 董好胜, 邓丽娟, 曹墨菊, 黄强. 一份玉米小籽粒发育突变体mn-like1的遗传分析与分子鉴定[J]. 作物学报, 2025, 51(6): 1569-1581. |
|
||