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作物学报 ›› 2013, Vol. 39 ›› Issue (06): 1039-1044.doi: 10.3724/SP.J.1006.2013.01039

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻颖壳退化突变体degraded hull 2 (dh2)的遗传分析与基因定位

郭爽**,李云峰**,任德勇,张天泉,何光华*   

  1. 西南大学水稻研究所 / 转基因植物与安全控制重庆市重点实验室,重庆 400716
  • 收稿日期:2012-10-31 修回日期:2013-01-15 出版日期:2013-06-12 网络出版日期:2013-02-19
  • 通讯作者: 何光华, E-mail: hegh@swu.edu.cn
  • 基金资助:

    本研究由国家高技术研究发展计划(863计划)项目(2011AA10A100),国家自然科学基金项目(31071390)和中央高校基本科研业务费专项资金(XDJK2012A001)资助。

Genetic Analysis and Gene Mapping of a degraded hull 2 (dh2) Mutant in Rice (Oryza sativa)

GUO Shuang**, LI Yun-Feng**, REN De-Yong, ZHANG Tian-Quan, and HE Guang-Hua*   

  1. Rice Research Institute, Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops, Southwest University, Chongqing 400716, China
  • Received:2012-10-31 Revised:2013-01-15 Published:2013-06-12 Published online:2013-02-19
  • Contact: 何光华, E-mail: hegh@swu.edu.cn

摘要:

鉴定和克隆水稻花器官突变体新基因,对了解水稻花器官发育的分子遗传机制和分子信号调控途径有着重要的作用。本研究报道了1个水稻颖壳异常突变体,来源于EMS (ethyl methane sulfonate)处理的缙恢10(Oryza sativa)诱变群体,暂被命名为degraded hull 2 (dh2)。表型分析发现突变体小花第一轮内稃或外稃横向细胞数目减少,导致内稃或外稃变窄而不能正常勾合,从而呈现开裂现象,其内三轮花器官均无明显变化。遗传分析表明该突变性状受1个隐性单基因控制。利用群体分离分析法(bulked segregation analysis, BSA),将DH2基因定位在第3染色体的IND-5IND-14之间,遗传距离分别为0.99 cM1.49 cM。该研究结果为DH2基因的图位克隆奠定了基础,对水稻花发育生物学研究具有重要的意义

关键词: 水稻, degraded hull 2 (dh2), 遗传分析, 基因定位

Abstract:

The identification and cloning of novel mutant genes of floral organ in rice play an important role in understanding the molecular genetic mechanisms and molecular signal pathways regulating floral organ development. A rice mutant, degraded hull 2(dh2), which was derived from ethylmethane sulfonate (EMS)-treated Jinhui 10 (Oryza sativa), exhibited defects in hull development. The dh2 floretsdisplayed open hull in whorl 1, however, the rest floral parts in other three whorls had no obvious change. Further analysis indicated that the number of transverse cells decreased, making the lemma or palea narrow, and causing the hull open. The genetic analysis revealed that the dh2 trait is controlled by a single recessive gene. Using the BSA method, the DH2 gene was finally mapped between IND-5 and IND-14 on chromosome 3 with geneticdistances of 0.99 cM and 1.49 cM, respectively. These results are useful for the map-based cloning of DH2 gene, and very important in the studies of floral development biology.

Key words: Rice (Oryza sativa), degraded hull 2 (dh2), Genetic analysis, Gene mapping

[1]Bowman J L, Smyth D R, Meyerowitz E M. Genetic interactionsamong floral homeotic genes of Arabidopsis. Development, 1991, 112: 1–20



[2]Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353: 31–37



[3]Ditta G, Pinyopich A, Robles P, Pelaz S, Yanofsky M F. The SEP4 gene of Arabidopsis thaliana functions in floral organ and meristem identity. Curr Biol, 2004, 14: 1935–1940



[4]Theissen G, Saedler H. Plant biology: Floral quartets. Nature, 2001, 409: 469–471



[5]Weigel D, Meyerowitz E M. The ABCs of floral homeotic genes. Cell, 1994, 78: 203–209



[6]Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, Nagato Y. SUPERWOMAN1, DROOPING LEAF genes control floral organ identity in rice. Development, 2003, 130: 705–718



[7]Whipple C J, Ciceri P, Padilla C M, Ambrose B A, Bandong S L, Schmidt R J. Conservation of B-class floral homeotic gene function between maize and Arabidopsis. Development, 2004, 131: 6083–6091



[8]Yamaguchi T, Lee D Y, Miyao A, Hirochika H, An G H, Hirano H Y. Functional diversification of the two C-class MADS-box genes OSMADS3 and OSMADS58 in Oryza sativa. Plant Cell, 2006, 18: 15–28



[9]Mandel M A, Brown C G, Savidge B, Yanofsky M F. Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature, 1992, 360: 273–277



[10]Drews G N, Bowman J L, Meyerowitz E M. Negative regulation of the Arabidopsis homeotic gene AGAMOUS by the APETALA2 product. Cell, 1991, 65: 991–1002



[11]Mizukami Y, Ma H. Ectopic expression of the floral homeotic gene agamous in transgenic Arabidopsis plants alters floral organ identity. Cell, 1992, 71: 119–131



[12]Xiao H, Tang J F, Li Y F, Wang W M, Li X B, Jin L, Xie R, Luo H F, Zhao X F, Meng Z, He G H, Zhu L H. STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice. Plant J, 2009, 59: 789–801



[13]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88: 9828–9832



[14]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8: 4321–4325



[15]Sang X-C(桑贤春), He G-H(何光华), Zhang Y(张毅), Yang Z-L(杨正林), Pei Y(裴炎). The simple gain of templates of rice genomes DNA for PCR. Hereditas (遗传), 2003, 25(6): 705–707 (in Chinese with English abstract)



[16]Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene RL10 (t) in rice (Oryza sativa L.). Genome, 2007, 50: 811–817



[17]Lander E S, Green P, Abrahamson J, Barlow A, Daly M J, Lincoln S E, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174–181



[18]Kosambi D D. The estimation of map distances from recombination values. Ann Eugen, 1944, 12: 172–175



[19]Prasad K, Parameswaran S, Vijayraghavan U. OsMADS1, a rice MADS-box factor, controls differentiation of specific cell types in the lemma and palea and is an early-acting regulator of inner floral organs, Plant J, 2005, 43: 915–928



[20]Jin Y, Luo Q, Tong H N, Wang A J, Cheng Z J, Tang J F, Li D Y, Zhao X F, Li X B, Wan J M, Jiao Y L, Chu C C, Zhu L H. An at-hook gene is required for palea formation and floral organ number control in rice. Dev Biol, 2011, 359: 277–288



[21]Agrawal K G, Abe K, Yamazaki M, Miyao A, Hirochika A. Conservation of the E-function for floral organ identity in rice revealed by the analysis of tissue culture-induced loss of function mutants of the OsMADS1 gene. Plant Mol Biol, 2005, 59: 125–135



[22]Chen Z X, Wu J G, Ding W N, Chen H M, Wu P, Shi C H. Morphogenesis and molecular basis on naked seed rice, a novel homeotic mutation of OsMADS1 regulating transcript level of AP3 homologue in rice. Planta, 2006, 223: 882–890



[23]Wang K J, Tang D, Hong L L, Xu W Y, Huang J, Li M, Gu M H, Xue Y B, Cheng Z K. DEP and AFO regulate reproductive habit in rice. PloS Genet, 2010, 6: e1000818



[24]Yuan Z, Gao S, Xue D W, Luo D, Li L T, Ding S Y, Yao X, Wilson Z A, Qian Q, Zhang D B. RETARDED PALEA1 controls palea development and floral zygomorphy in rice. Plant Physiol, 2009, 149: 235–244



[25]Shinnosuke O, Mayumi K, Maiko S, Akio M, Hirohiko H, Eiji U, Yasuo N, Hitoshi Y. MOSAIC FLORAL ORGANS 1, an AGL6-like MADS box gene, regulates floral organ identity and meristem fate in rice. Plant Cell, 2009, 21: 3008–3025



[26]Sang X C, Li Y F, Luo Z K, Ren D Y, Fang L K, Wang N, Zhao F M, Ling Y H, Yang Z L, Liu Y S, He G H. CHIMERIC FLORAL ORGANS 1, encoding a Monocot-specific MADS-box protein, regulates floral organ identity in rice. Plant Physiol, 2012, 160: 788–807



[27]Zhang Y-Z(张玉烛), Zhang G-H(张桂和), Zhu G-C(朱国才), Deng Q-Y(邓启云), Zhan Q-C(詹庆才). Effects of overcast and raining on flowering, fertilizing and seed setting of early rice. Chin J Rice Sci (中国水稻科学), 1995, 9(3): 173–178 (in Chinese with English abstract)



[28]Wang Z(王忠), Gu Y-J(顾蕴洁), Yu H-L(于洪亮), Shi H-Y(石火英), Gao Y-Z(高煜珠). Studies on the cause of formation of deformed kernel of wild abortion type male sterile line in rice. Sci Agric Sin (中国农业科学), 1995, 28(6): 25–31 (in Chinese with English abstract)

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