作物学报 ›› 2013, Vol. 39 ›› Issue (12): 2107-2114.doi: 10.3724/SP.J.1006.2013.02107
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
孙翠花1,侯丽媛1,郭慧娟2,张晓军2,*,贾举庆3,李欣2,詹海仙2,畅志坚2,*
SUN Cui-Hua1,HOU Li-Yuan1,GUO Hui-Juan2,ZHANG Xiao-Jun2,*,JIA Ju-Qing3,LI Xin2,ZHAN Hai-Xian2,CHANG Zhi-Jian2,*
摘要:
小麦新种质CH09W83为八倍体小偃麦TAI7047与高感小麦品种晋太170杂交、回交后代衍生而来的高代选系,在苗期免疫或高抗我国白粉病菌株E09、E20、E21、E23、E26、Bg1和Bg2。为定位CH09W83中的抗病基因,将CH09W83与感病亲本杂交和回交,通过对F1、F2、F2:3和BC1代的接种鉴定和遗传分析,证实CH09W83成株期对E09的抗性由1对隐性核基因控制,暂命名为pmCH83。采用分离群体分组分析法(bulked segregant analysis, BSA),以658对SSR标记对台长29(感病)× CH09W83的F2群体分析发现,抗性基因pmCH83与SSR标记Xgpw7272、Xwmc652、Xgwm251、Xgwm193连锁,与两翼邻近标记Xwmc652和Xgwm251的遗传距离分别为3.8 cM和4.3 cM。利用中国春缺体–四体、双端体将pmCH83及其连锁标记定位在4BL染色体上。原位杂交、染色体配对及连锁标记分析结果表明,CH09W83可能是一个小麦与中间偃麦草的隐形异源渗入系。系谱和图谱位置分析表明,pmCH83很可能是来自中间偃麦草一个新的抗白粉病基因。
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