作物学报 ›› 2013, Vol. 39 ›› Issue (12): 2115-2122.doi: 10.3724/SP.J.1006.2013.02115
杨艳歌1,2,吕维涛2,孙冬梅1,*,凌毅3,*,邓馨2,*
YANG Yan-Ge1,2,LÜ Wei-Tao2,SUN Dong-Mei1,*,LING Yi3,*,DENG Xin2,*
摘要:
碱性亮氨酸拉链(basic leucine zipper, bZIP)是真核生物特有的转录因子家族,在高等植物基因表达与调控中起重要作用。本文通过对玉米基因组数据库中收录的全长cDNA序列全基因组范围的bZIP分析,发现其中25个序列编码D亚族bZIP转录因子。针对这些序列进行生物信息学分析、染色体分布和直系同源组的分类分析,发现部分序列可能是由同一基因座编码,但不同方式剪切形成的。对部分基因克隆和测序发现了更多的剪切形式。定量检测其中3个基因在ABA和干旱胁迫条件下的表达发现,其中1个受ABA诱导,2个受ABA抑制,但均不受干旱胁迫影响。表明玉米中D亚族基因可能参与ABA响应。
| [1]Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez M M, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K. AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell, 2005, 17: 3470–3488[2]Siberil Y, Doireau P, Gantet P. Plant bZIP G-box binding factors. Modular structure and activation mechanisms. Eur J Biochem, 2001, 268: 5655–5666[3]Mehrotra R, Kiran K, Chaturvedi CP, Ansari SA, Lodhi N, Sawant S, Tuli R. Effect of copy number and spacing of the ACGT and GT cis elements on transient expression of minimal promoter in plants. J Genet, 2005, 84: 183–187[4]Jakoby M, Weisshaar B, Droge-Laser W, Vicente-Carbajosa J, Tiedemann J, Kroj T, Parcy F, Grp bR. bZIP transcription factors in Arabidopsis. Trends Plant Sci, 2002, 7: 106–111[5]Correa L G, Riano-Pachon D M, Schrago C G, dos Santos R V, Mueller-Roeber B, Vincentz M. The role of bZIP transcription factors in green plant evolution: adaptive features emerging from four founder genes. PloS ONE, 2008, 3: e2944[6]Chuang C F, Running M P, Williams R W, Meyerowitz E M. The PERIANTHIA gene encodes a bZIP protein involved in the determination of floral organ number in Arabidopsis thaliana. Genes Dev, 1999, 13: 334–344[7]Walsh J, Freeling M. The liguleless2 gene of maize functions during the transition from the vegetative to the reproductive shoot apex. Plant J, 1999, 19: 489–495[8]Foley R C, Singh K B. TGA5 acts as a positive and TGA4 acts as a negative regulator of ocs element activity in Arabidopsis roots in response to defence signals. Febs Lett, 2004, 563: 141–145[9]Zhou J M, Trifa Y, Silva H, Pontier D, Lam E, Shah J, Klessig D F. NPR1 differentially interacts with members of the TGA/OBF family of transcription factors that bind an element of the PR–1 gene required for induction by salicylic acid. Mol Plant Microbe Interact, 2000, 13: 191–202[10]Johnson C, Boden E, Arias J. Salicylic acid and NPR1 induce the recruitment of trans-activating TGA factors to a defense gene promoter in Arabidopsis. Plant Cell, 2003, 15: 1846–1858[11]Gatz C. From pioneers to team players. TGA transcription factors provide a molecular link between different stress pathways. Mol Plant Microbe Interact, 2013, 26: 151–159[12]Xiang C, Miao Z, Lam E. DNA-binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana. Plant Mol Biol, 1997, 34: 403–415[13]Foley R C, Grossman C, Ellis J G, Llewellyn D J, Dennis E S, Peacock W J, Singh K B. Isolation of a maize bZIP protein subfamily: candidates for the ocs-element transcription factor. Plant J, 1993, 3: 669–679[14]Schindler U, Menkens A E, Beckmann H, Ecker J R, Cashmore A R. Heterodimerization between light-regulated and ubiquitously expressed Arabidopsis GBF bZIP proteins. EMBO J, 1992, 11: 1261–1273[15]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C) method. Methods, 2001, 25: 402–408[16]Chenna R, Sugawara H, Koike T, Lopez R, Gibson T J, Higgins D G, Thompson J D. Multiple sequence alignment with the clustal series of programs. Nucl Acids Res, 2003, 31: 3497–3500[17]Kumar S, Tamura K, Nei M. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform, 2004, 5: 150–163[18]Bentsink L, Jowett J, Hanhart C J, Koornneef M. Cloning of DOG1, a quantitative trait locus controlling seed dormancy in Arabidopsis. Proc Natl Acad Sci USA, 2006, 103: 17042–17047[19]Yoshida T, Fujita Y, Sayama H, Kidokoro S, Maruyama K, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K. AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J, 2010, 61: 672–685[20]Graeber K, Linkies A, Muller K, Wunchova A, Rott A, Leubner-Metzger G. Cross-species approaches to seed dormancy and germination: conservation and biodiversity of ABA-regulated mechanisms and the Brassicaceae DOG1 genes. Plant Mol Biol, 2010, 73: 67–87[21]Chiang G C, Barua D, Dittmar E, Kramer E M, de Casas R R, Donohue K. Pleiotropy in the wild: the dormancy gene DOG1 exerts cascading control on life cycles. Evolution, 2013, 67: 883–893[22]Li X(李昕), Yang S(杨爽), Peng L-X(彭立新), Wang W(王文). The origin and evolution of new genes. Chin Sci Bull (科学通报), 2004, 49(13): 1219–1225 (in Chinese) |
| [1] | 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901. |
| [2] | 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801. |
| [3] | 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308. |
| [4] | 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325. |
| [5] | 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590. |
| [6] | 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352. |
| [7] | 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500. |
| [8] | 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364. |
| [9] | 蔡宏玮, 于爱忠, 姜科强, 王鹏飞, 王玉珑, 霍建喆, 庞小能, 尹波, 尚永盼. 干旱灌区有机肥替代部分化肥促进甜玉米产量提升的关键机制[J]. 作物学报, 2026, 52(4): 1166-1180. |
| [10] | 田红丽, 杨扬, 范亚明, 易红梅, 郭丹丹, 王凤格, 赵久然. 适于玉米品种鉴定的一套三等位变异SNP新型标记组合[J]. 作物学报, 2026, 52(4): 993-1005. |
| [11] | 杨亚莉, 徐明睿, 马越飞, 海艺蕊, 刘凯栋, 刘万茂, 孙颖. 玉米根尖及整根响应缺铁的转录组比较研究[J]. 作物学报, 2026, 52(4): 1006-1021. |
| [12] | 张超, 郭欢, 李忠玲, 岳淑宁, 赵娜. 基于BSA-seq技术定位玉米籽粒花青素关联基因[J]. 作物学报, 2026, 52(3): 780-789. |
| [13] | 郭向阳, 涂亮, 王栋, 刘鹏飞, 王安贵, 易强, 任洪, 李刚, 祝云芳, 吴迅, 蒋喻林, 田丰, 陈泽辉. 热带Suwan种质在我国玉米种质改良中的创新与利用[J]. 作物学报, 2026, 52(3): 655-664. |
| [14] | 孟成, 王哲. 玉米ZmPFK基因家族全基因组鉴定及响应胁迫表达分析[J]. 作物学报, 2026, 52(3): 764-779. |
| [15] | 李新浩, 邢梦柯, 周梓惠, 李思烨, 任昊, 王洪章, 赖华江. 外源褪黑素通过协调光反应与暗反应增强玉米苗期的耐热性[J]. 作物学报, 2026, 52(3): 839-856. |
|
||