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作物学报 ›› 2013, Vol. 39 ›› Issue (12): 2107-2114.doi: 10.3724/SP.J.1006.2013.02107

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

小麦–中间偃麦草隐形渗入系抗白粉病基因pmCH83分子定位

孙翠花1,侯丽媛1,郭慧娟2,张晓军2,*,贾举庆3,李欣2,詹海仙2,畅志坚2,*   

  1. 1 山西大学研究生院,山西太原 030006;2 山西省农业科学院作物科学研究所 / 农业部黄土高原作物基因资源与种质创制重点实验室,山西太原 030031;3 山西农业大学农学院,山西太谷 030801
  • 收稿日期:2013-05-17 修回日期:2013-07-25 出版日期:2013-12-12 网络出版日期:2013-09-29
  • 通讯作者: 畅志坚, E-mail: wrczj@126.com; 张晓军, E-mail: zxjemail@163.com
  • 基金资助:

    本研究由国家自然科学基金项目(31171839), 山西省国际科技合作计划项目(2012081006-2; 2013081007), 山西省科技攻关项目(20130311001-5)和山西省回国留学人员科研项目(2012-102)资助。

Molecular Mapping of Powdery Mildew Resistance Gene pmCH83 in a Putative Wheat–Thinopyrum intermedium Cryptic Introgression Line

SUN Cui-Hua1,HOU Li-Yuan1,GUO Hui-Juan2,ZHANG Xiao-Jun2,*,JIA Ju-Qing3,LI Xin2,ZHAN Hai-Xian2,CHANG Zhi-Jian2,*   

  1. 1 Graduate School of Shanxi University, Taiyuan 030006, China; 2 Institute of Crop Science, Shanxi Academy of Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Taiyuan 030031, China; 3 College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
  • Received:2013-05-17 Revised:2013-07-25 Published:2013-12-12 Published online:2013-09-29
  • Contact: 畅志坚, E-mail: wrczj@126.com; 张晓军, E-mail: zxjemail@163.com

摘要:

小麦新种质CH09W83为八倍体小偃麦TAI7047与高感小麦品种晋太170杂交、回交后代衍生而来的高代选系,在苗期免疫或高抗我国白粉病菌株E09E20E21E23E26Bg1Bg2为定位CH09W83中的抗病基因,将CH09W83与感病亲本杂交和回交,通过对F1F2F2:3BC1代的接种鉴定和遗传分析,证实CH09W83成株期对E09的抗性由1对隐性核基因控制,暂命名为pmCH83采用分离群体分组分析法(bulked segregant analysis, BSA),以658SSR标记对台长29(感病CH09W83F2群体分析发现,抗性基因pmCH83SSR标记Xgpw7272Xwmc652Xgwm251Xgwm193连锁,与两翼邻近标记Xwmc652Xgwm251的遗传距离分别为3.8 cM4.3 cM。利用中国春缺体四体、双端体将pmCH83及其连锁标记定位在4BL染色体上。原位杂交、染色体配对及连锁标记分析结果表明,CH09W83可能是一个小麦与中间偃麦草的隐形异源渗入系。系谱和图谱位置分析表明,pmCH83很可能是来自中间偃麦草一个新的抗白粉病基因。

关键词: 隐形异源渗入, 白粉病抗性, 连锁图谱, 分子标记, GISH

Abstract:

Wheat introgression line CH09W83 is immune or highly resistant to wheat powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) pathotypes E09, E20, E21, E23, E26, Bg1, and Bg2 at seedling stage in China. This line was derived from the cross between wheat–Thinopyrum intermedium line TAI7047 (resistant) and wheat cultivar Jintai 170 (susceptible). To identify the Bgt resistance gene(s) in CH09W83, we developed segregating populations (F2, F2:3, and BC1) by crossing/backcrossing CH09W83 with susceptible wheat parents. The result of genetic analysis showed that the adult resistance to Bgt E09 in CH09W83 was controlled by a single recessive gene, which was tentatively designated as pmCH83. Using the F2 population of Taichang 29 (susceptible) ´ CH09W83 and 658 pairs of SSR primers, four codominant SSR markers, i.e., Xgwm193, Xgwm251, Xwmc652, and Xgpw7272, were identified to be linked with pmCH83 according to bulked segregant analysis, and the genetic distances between the target gene and flanking markers were 4.3 cM to Xgwm251 and 3.8 cM to Xwmc652. Using Chinese Spring nullitetrasomic and ditelosomic lines, pmCH83 was located on chromosomal arm 4BL of wheat. In combination with evidence of bivalent pairing at metaphase I and GISH, CH09W83 is probably a cryptic wheat–Th. intermedium translocation. Based on pedigree analysis and linkage map, we deduced that pmCH83 is a novel Bgt resistance gene originating from Th. intermedium.

Key words: Cryptic alien introgression, Resistance to powdery mildew, Linkage map, Molecular markers, GISH

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