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作物学报

作物学报 ›› 2014, Vol. 40 ›› Issue (10): 1711-1716.doi: 10.3724/SP.J.1006.2014.01711

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

水稻类树状突变体s2-21的遗传分析与精细定位

王海凤1,高洁1,孙伟1,张士永1,赵庆雷1,尹亮1,赵金凤2,李学勇2,*,袁守江1,*   

  1. 1山东省水稻研究所，山东济南250100；2中国农业科学院作物科学研究所 / 作物基因资源与基因改良国家重大科学工程，北京100081
  • 收稿日期:2014-03-14 修回日期:2014-07-06 出版日期:2014-10-12 网络出版日期:2014-07-25
  • 通讯作者: 李学勇, E-mail: lixueyong@caas.cn, Tel: 010-82107409; 袁守江, E-mail: ysj868@sina.com, Tel: 15615973691
  • 基金资助:

    本研究由国家重点基础研究发展计划(973计划)项目(2013CBA01401)和国家转基因生物新品种培育重大专项(2013ZX08009003)资助。

Genetic Analysis and Gene Fine Mapping of Rice Leafy Head Mutant s2-21

WANG Hai-Feng1,GAO Jie1,SUN Wei1,ZHANG Shi-Yong1,ZHAO Qing-Lei1,YIN Liang1,ZHAO Jin-Feng2,LI Xue-Yong2,*,YUAN Shou-Jiang1,*   

  1. 1 Shandong Rice Research Institute, Jinan 250100, China; 2 National Key Facility for Crop Gene Resource and Geneic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2014-03-14 Revised:2014-07-06 Published:2014-10-12 Published online:2014-07-25
  • Contact: 李学勇, E-mail: lixueyong@caas.cn, Tel: 010-82107409; 袁守江, E-mail: ysj868@sina.com, Tel: 15615973691

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摘要：

叶序和出叶间隔期是叶片生长发育的基本生物学特性和水稻的重要农艺性状之一。对叶序或出叶间隔期突变体的研究，可以帮助我们了解叶片的形成机制。本研究以甲基磺酸乙酯(EMS)诱变粳稻品种日本晴，获得一个稳定遗传的类树状突变体s2-21。该突变体出叶间隔期变短、节间缩短、植株矮化、分蘖数减少、叶片数增加、不能正常进行生殖生长。将该突变体与籼稻品种Dular杂交，遗传分析表明该突变体性状受1对隐性基因控制。通过InDel分子标记对s2-21/Dular F2群体进行遗传定位，将该基因初步定位在第1染色体InDel标记C1-15和S1-17之间。利用本实验已测序的籼稻品种Dular全基因组序列与NCBI (http://www.ncbi.nlm.nih.gov/)上提供的粳稻品种日本晴基因组序列比对，发展了6个新的InDel标记，最终将该基因定位在W25和W26之间约88 kb的区间内。测序结果表明该突变体中PLA2基因的第4个内含子的第5位碱基由G突变为A。

关键词: 水稻, 类树状突变体, 出叶间隔期, 基因定位, PLA2

Abstract:

Phyllotaxy and plastochron are basic aspects of leaf development and the important agronomic traits of rice .The study of phyllotaxy or plastochron mutant will help us to understand the mechanism of leaf formation. In this study, a leafy head mutant s2-21 was isolated from an EMS (ethyl methane sulfonate) mutagenized japonica cultivar Nipponbare. It was characterized by shorter intervals between leaves, shortened internodes, dwarf plants, decreased tiller number, and increased leaf number. It was also unable to transit from vegetative to reproductive growth. Genetic analysis with a F2 population of the mutant and an indica cultivar Dular showed that this trait is controlled by a single recessive nucleus gene, which was primarily mapped between two insertion-deletion (InDel) markers C1-15 and S1-17 on chromosome 1. For the fine mapping, new InDel markers were designed by utilizing information of genomic sequences from Nipponbare (http://www.ncbi.nlm.nih.gov/) and Dular (our unpublished data), and eventually the gene was localized within the region of 88 kb between W25 and W26. Sequence analysis indicated the mutant was caused by mutation of the fifth base from G to A in the fourth intron of the pla2 gene.

Key words: Rice, Leafy head mutant, Plastochron, Gene mapping, PLA2

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