玉米种子休眠相关蛋白的鉴定与分析

doi:10.3724/SP.J.1006.2014.01865

摘要/Abstract

摘要：

以强休眠性玉米自交系08-641为材料, 2个弱休眠性玉米自交系为对照, 利用蛋白质双向电泳技术, 对处于休眠状态下的新鲜收获种子和经过15 d后熟处理破除休眠的3个自交系种子进行了蛋白质差异表达分析。结果表明, 在3次重复试验下共检测到9个与休眠相关的蛋白点, 其中新增诱导表达蛋白质1个, 缺失表达蛋白质2个, 上调表达蛋白质5个, 下调表达蛋白质1个。在9个蛋白质中, 有5个蛋白点得到鉴定。包括3个globulin-1 S allele precursor、1个2-isopropylmalate synthase B和1个translationally-controlled tumor protein。种子休眠破除过程中的蛋白质的变化说明种子经历了一系列生理生化活动, 深入研究这些蛋白质的生物学功能将有助于更清楚地认识玉米种子休眠问题。

关键词: 玉米, 种子休眠, 贮藏物质, 双向电泳, 蛋白质组

Abstract:

Because the special natural climate of overabundant rain and poor sunshine in the southwest, pre-harvest sprouting has seriously affected the quality and yield of maize, looking for a good material with resistance to pre-harvest sprouting is the urgent need for improvement of existing maize strains. The results of this study indicated that proteome of 08-641, 008, and 127 seeds significantly changed during afterripening. Nine differentially expressed proteins related to dormancy in the seeds of maize inbred line 08-641 with 15 d afterripening process were analysed through 2-DE, including one newly induced expressed protein, two missing expressed proteins, five up-regulated expressed proteins, and one down-regulated expressed protein. Among the nine proteins,five proteins were identified by mass spectrometry, including three globulin-1 S allele precursor of storage proteins,one 2-isopropylmalate synthase B and one translationally-controlled tumor protein involved in regulating protein structure and cell function. The changes of proteins during dry ripening process of seeds indicated that the seeds undergo a series of physiological and biochemical activities befor they can germinate normally, and the deeply study on the biological function of these proteins will help us understand maize seed dormancy more clearly.

Key words: Maize, Seed dormancy, Storage substances, Two dimensional electrophoresis, Proteome

兰海,冷亦峰,周树峰,刘坚,荣廷昭. 玉米种子休眠相关蛋白的鉴定与分析[J]. 作物学报, 2014, 40(10): 1865-1871.

LAN Hai,LENG Yi-Feng,ZHOU Shu-Feng,LIU Jian,RONG Ting-Zhao. Proteomic Analysis of Dormant Seeds with Dry Ripening Process in Maize Inbred Lines[J]. Acta Agron Sin, 2014, 40(10): 1865-1871.

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