作物学报 ›› 2015, Vol. 41 ›› Issue (01): 57-65.doi: 10.3724/SP.J.1006.2015.00057
温娟1,许剑锋1,龙艳2,徐海明1,孟金陵2,吴建国3,*,石春海1,*
WEN Juan1, XU Jian-Feng1, LONG Yan2, XU Hai-Ming1, MENG Jin-Ling2, WU Jian-Guo3,*,SHI Chun-Hai1,*
摘要:
菜籽饼是重要的饲料蛋白质来源,氨基酸组成与饲料营养品质有着密切关系,其中丝氨酸、胱氨酸和酪氨酸为多数动物的半必需氨基酸。本研究利用甘蓝型油菜双单倍体(DH)群体分别与双亲Tapidor和Ningyou 7回交构建的2套BC1F1群体,采用新创建的双子叶作物种子品质性状遗传体系QTL定位软件和作图方法,对油菜籽丝氨酸、胱氨酸和酪氨酸含量进行了种子胚和母体植株2套遗传体系的QTL定位分析。结果表明,在A1、A4、A7、A8、A9、C2、C3和C9染色体上检测到5个丝氨酸含量QTL、2个胱氨酸含量QTL和5个酪氨酸含量QTL,分别能解释59.34%、29.66%和59.26%的表型变异。其中5个QTL属于主效QTL,均能解释10%以上的表型变异。全部QTL均具极显著的胚和母体加性主效应,其中3个QTL具显著或极显著的环境互作效应。在A4染色体上发现1个QTL簇,该区域存在3个控制丝氨酸、胱氨酸和酪氨酸含量的QTL。一些重要QTL以及与之紧密连锁的分子标记在今后图位克隆和分子标记辅助选择育种中具有重要的利用价值。
| [1]王绍中, 李春喜, 罗艳蕊, 姜丽娜. 基因型和地域分布对小麦籽粒氨基酸含量影响的研究. 西北植物学报, 2001, 21: 437–445Wang S Z, Li C X, Luo Y R, Jiang L N. Investigation on effects for genotypes and region distribution to the grain amino acid contents of winter wheat. Acta Bot Boreal-Occident Sin, 2001, 21: 437–445 (in Chinese with English abstract)[2]Muncka L, Pram N J, Moller B, Jacobsen S, Sondergaard I, Engelsen S B, Norgaard L, Broa R. Exploring the phenotypic expression of a regulatory proteome-altering gene by spectroscopy and chemometrics. Analytica Chimica Acta, 2001, 446: 171–186[3]Bell J M, Rakow G, Downey R K. Comparisons of amino acid and protein levels in oil-extraeted seeds of Brassica and Sinapis species, with observations on environmental effects. Can J Anim Sci, 2000, 80: 169–174[4]任玉玲, 石春海, 吴建国, 张海珍. 油菜籽三种氨基酸含量的胚、细胞质和母体遗传效应分析. 浙江大学学报(农业与生命科学版), 2005, 31: 41–46Ren Y L, Shi C H, Wu J G, Zhang H Z. Genetic analysis of embryo, cytoplasmic and maternal effects on three amino acid traits in rapeseed. J Zhejiang Univ (Agric Life Sci), 2005, 31: 41–46 (in Chinese with English abstract)[5]Zeng Z B. Theoretical basis for separation of multiple linked gene effects in mapping quantitative trait loci. Proc Natl Acad Sci USA, 1993, 90: 10972–10976[6]Jansen R C, Stam P. High resolution of quantitative traits into multiple loci via interval mapping. Genetics, 1994, 136: 1447–1455[7]Zhu J, Weir B S, Chen L S. Mixed Model Approaches for Genetic Analysis of Quantitative Traits. In: Proceedings of the International Conference on Mathematical Biology. Singapore: World Scientific Publishing Co., 1998. pp 321–330[8]Shi C H, Shi Y, Lou X Y, Xu H M, Zheng X, Wu J G. Identification of endosperm and maternal plant QTLs for protein and lysine contents of rice across different environments. Crop Pasture Sci, 2009, 60: 295–301[9]Panthee D R, Pantalone V R, Sams C E, Saxton A M, West D R, Orf J H, Killam A S. Quantitative trait loci controlling sulfur containing amino acids, methionine and cysteine, in soybean seeds. Theor Appl Genet, 2006, 112: 546–553[10]Jiang X L, Deng Z Y, Ru Z G, Wu P, Tian J C. Quantitative trait loci controlling amino acid contents in wheat (Triticum aestivum L.). Aust J Crop Sci, 2013, 7: 820–829[11]Liu H Y, Quampah A, Chen J H, Li J R, Huang Z R, He Q L, Zhu S J, Shi C H. QTL Mapping based on different genetic systems for essential amino acid contents in cottonseeds in different environments. PLoS One, 2013, 8: e57531[12]Xu J F, Long Y, Wu J G, Xu H M, Wen J, Meng J L, Shi C H. QTL mapping and analysis of the embryo and maternal plant for three limiting amino acids in rapeseed meal. Eur Food Res Technol, 2014[13]Zhang H Z, Shi C H, Wu J G, Ren Y L, Li C T, Zhang D Q, Zhang Y F. Analysis of genetic and genotype × environment interaction effects from embryo, cytoplasm and maternal plant for oleic acid content of Brassica napus L. Plant Sci, 2004, 167: 43–48[14]Wu J G, Shi C H, Zhang H Z. Genetic analysis of embryo, cytoplasmic and maternal effects and their environment interactions for protein content in Brassica napus L. Aust J Agr Res, 2005, 56: 69–73[15]Variath M T, Wu J, Zhang L, Shi C H. Analysis of developmental genetic effects from embryo, cytoplasm and maternal plant for oleic and linoleic acid contents of rapeseed. J Agric Sci, 2010, 148: 375–391[16]Yang J, Zhu J, Williams R W. Mapping the genetic architecture of complex traits in experimental populations. Bioinformatics, 2007, 23: 1527–1536[17]郑希, 吴建国, 楼向阳, 徐海明, 石春海. 不同环境条件下稻米组氨酸和精氨酸的胚乳和母体植株QTL分析. 作物学报, 2008, 34: 369–375Zheng X, Wu J G, Lou X Y, Xu H M, Shi C H. Mapping and analysis of QTLs on maternal and endosperm genomes for histidine and arginine in rice (Oryza Sativa L.) across environments. Acta Agron Sin, 2008, 34: 369–375 (in Chinese with English abstract)[18]Liu H Y, Quampah A, Chen J H, Li J R, Huang Z R, He Q L, Shi C H, Zhu S J. QTL analysis for gossypol and protein contents in upland cottonseeds with two different genetic systems across environments. Euphytica, 2012, 188: 453–463[19]Qiu D, Morgan C, Shi J, Long Y, Liu J, Li R, Zhuang X, Wang Y, Tan X, Dietrich E, Weihmann T, Everett C, Vanstraelen S, Beckett P, Fraser F, Trick M, Barnes S, Wilmer J, Schmidt R, Li J, Li D, Meng J, Bancroft I. A comparative linkage map of oilseed rape and its use for QTL analysis of seed oil and erucic acid content. Theor Appl Genet, 2006, 114: 67–80[20]Chen G L, Zhang B, Wu J G, Shi C H. Nondestructive assessment of amino acid composition in rapeseed meal based on intact seeds by near-infrared reflectance spectroscopy. Anim Feed Sci Tech, 2011, 165: 111–119[21]Shi J Q, Li R L, Qiu D, Jiang C C, Long Y, Morgan C, Bancroft I, Zhao J Y, Meng J L. Unraveling the complex trait of crop yield with quantitative trait loci mapping in Brassica napus. Genet, 2009, 182: 851–861[22]McCouch S R, Cho Y G, Yano P E, Blinstrub M, Morishima H, Kinoshita T. Report on QTL nomenclature. Rice Genet Newslett, 1997, 14: 11–13[23]Falconer D S, Mackay T F C. Introduction to Quantitative Genetics. 4th Edn. UK: Longmans Green, Harlow, Essex, 1996[24]Paterson A H, Lander E S, Hewitt J D, Peterson S, Lincoln S E, Tanksley S D. Resolution of quantitative traits into Mendelian factors using a complete linkage map of restriction fragment length polymorphisms. Nature, 1988, 335: 721–726[25]Tanksley S D. Mapping polygenes. Annu Rev Genet, 1993, 27: 205–233[26]Subhadra S, Mohapatra T, Rakesh S, Hussain Z. Mapping of QTLs for oil content and fatty acid composition in Indian mustard [Brassica juncea (L.) Czern. and Coss.]. J Plant Biochem Biot, 2013, 22: 80–89[27]Mahmood T, Rahman M H, Stringam G R, Yeh F, Good A G. Identification of quantitative trait loci (QTL) for oil and protein contents and their relationships with other seed quality traits in Brassica juncea. Theor Appl Genet, 2006, 113: 1211–1220[28]梅德圣, 张垚, 李云昌, 胡琼, 李英德, 徐育松. 油菜油分、蛋白质和硫苷含量相关性分析及 QTL定位. 植物学报, 2009, 44: 536–545Mei D S, Zhang Y, Li Y C, Hu Q, Li Y D, Xu Y S. Identification of quantitative trait loci for oil, protein and glucosinolate content in Brassica napus. Chin Bull Bot, 2009, 44: 536–545 (in Chinese with English abstract)[29]Zhang J F, Qi C K, Pu H M, Chen S, Chen F, Gao J Q, Chen X J, Gu H, Fu S Z. QTL identification for fatty acid content in rapeseed (Brassica napus L.). Acta Agron Sin, 2008, 34: 54–60[30]Yan X Y, Li J N, Wang R, Jin M Y, Chen L, Qian W, Wang X N, Liu L Z, Mapping of QTLs controlling content of fatty acid composition in rapeseed (Brassica napus). Genes Genom, 2011, 33: 365–371 |
| [1] | 郑玉珍, 齐飞艳, 孙子淇, 刘华, 秦利, 石磊, 王娟, 汪蒙蒙, 韩锁义, 徐静, 苗利娟, 黄冰艳, 董文召, 郑峥, 张新友. 花生籽仁总超长链脂肪酸和7种脂肪酸组分的QTL定位[J]. 作物学报, 2026, 52(6): 1646-1657. |
| [2] | 刘长友, 王珅, 时会影, 沈颖超, 孙蕾, 王彦, 张志肖, 苏秋竹, 田静, 范保杰. 基于饭豆基因资源的小豆远缘杂交群体抗豆象QTL定位[J]. 作物学报, 2026, 52(3): 936-944. |
| [3] | 马毅娜, 吴晓明玉, 李藕琪, 王圆, 陈丽, 张盈川, 赵伦, 文静, 傅廷栋, 沈金雄. Bna-miR1040-EIF3A模块调控油菜开花时间的功能研究[J]. 作物学报, 2026, 52(2): 349-362. |
| [4] | 王彬, 蒙姜宇, 邱浩良, 贺亚军, 钱伟. 甘蓝型油菜BnaDUF579基因家族的鉴定与表达模式分析[J]. 作物学报, 2025, 51(8): 2100-2110. |
| [5] | 宋改利, 王璐倩, 屈柯飞, 唐建卫, 董纯豪, 黄振朴, 高艳, 牛吉山, 殷贵鸿, 李巧云. Bipolaris sorokiniana黑胚病对中筋小麦淀粉含量、粒度分布与糊化特性的影响[J]. 作物学报, 2025, 51(8): 2164-2175. |
| [6] | 胡蒙, 沙丹, 张晟瑞, 谷勇哲, 张世碧, 李静, 孙君明, 邱丽娟, 李斌. 大豆分枝数QTL定位及候选基因筛选[J]. 作物学报, 2025, 51(7): 1747-1756. |
| [7] | 邵顺伟, 陈卓, 兰振东, 蔡兴奎, 邹华芬, 李晨曦, 唐景华, 朱熙, 张彧, 董建科, 金辉, 宋波涛. 基于BSA-seq技术的块茎芽眼深度QTL定位分析[J]. 作物学报, 2025, 51(7): 1725-1735. |
| [8] | 杨海洋, 吴林宣, 李博纹, 石翰峰, 袁禧龙, 刘金朝, 蔡海荣, 陈诗怡, 郭涛, 王慧. 基于QTL定位发现的OsWRI3调控水稻种子的落粒性[J]. 作物学报, 2025, 51(7): 1712-1724. |
| [9] | 张金泽, 周庆国, 肖莉晶, 金海润, 欧阳青静, 龙旭, 晏中彬, 田恩堂. 芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析[J]. 作物学报, 2025, 51(5): 1166-1177. |
| [10] | 夏琦, 郭滢, 王坤美, 王思忆, 巨建业, 彭雅雯, 刘忠松, 夏石头. 甘蓝型油菜种子和种皮中水杨酸含量与原花色素积累的关系研究[J]. 作物学报, 2025, 51(5): 1189-1197. |
| [11] | 宋松泉, 唐翠芳, 程红焱, 王程亮, 袁良兵, 左胜. 谷类作物的胚乳发育及其对种子休眠与萌发的作用[J]. 作物学报, 2025, 51(5): 1133-1155. |
| [12] | 朱建平, 李文奇, 许扬, 王芳权, 李霞, 蒋彦婕, 范方军, 陶亚军, 陈智慧, 吴莹莹, 杨杰. 水稻粉质胚乳突变体we2的表型分析与基因定位[J]. 作物学报, 2025, 51(4): 1110-1117. |
| [13] | 王晓琳, 刘忠松, 康雷, 杨柳. 甘蓝型油菜角果长度和每角粒数基因定位以及角果皮转录组动态分析[J]. 作物学报, 2025, 51(4): 888-899. |
| [14] | 张琴, 戴成, 马朝芝. 生长素响应报告基因转化甘蓝型油菜及各组织GUS动态信号分析[J]. 作物学报, 2025, 51(3): 667-675. |
| [15] | 孙程明, 周晓婴, 陈锋, 张维, 王晓东, 彭琦, 郭月, 高建芹, 胡茂龙, 付三雄, 张洁夫. 长链非编码RNA (lncRNA)在甘蓝型油菜分枝角度调控中的功能分析与预测[J]. 作物学报, 2025, 51(3): 559-567. |
|
||