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作物学报 ›› 2016, Vol. 42 ›› Issue (01): 31-42.doi: 10.3724/SP.J.1006.2016.00031

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

聚合稻瘟病、白叶枯病和褐飞虱抗性基因的三系恢复系改良效果的评价

楼珏1,杨文清1,李仲惺2,罗天宽1,谢永楚1,郑国楚1,岳高红1,徐建龙3,卢华金1,*   

  1. 1 温州市农业科学研究院 / 浙南作物育种重点实验室,浙江温州 325006;2 温州市植物保护站,浙江温州 325000;3 中国农业科学院作物科学研究所 / 农作物基因资源与遗传改良国家重大科学工程,北京 100081
  • 收稿日期:2015-06-12 修回日期:2015-09-06 出版日期:2016-01-12 网络出版日期:2015-10-13
  • 通讯作者: 卢华金, E-mail: luhjzw@163.com
  • 基金资助:

    本研究由浙江省农业科研重大专项(“8812”计划,2012C12901-17),温州市种子种苗科技创新专项(N20120019),浙江省教育科学规划课题(2015SCG183)和和浙江省教育厅一般科研项目(Y201533916)资助。

Evaluation of Improvement Effect of Restorer Lines on Pyramiding Genes Resistant to Rice Blast, Bacterial Leaf Blight and Brown Planthopper

LOU Jue1,YANG Wen-Qing1,LI Zhong-Xing2,LUO Tian-Kuan1,XIE Yong-Chu1,ZHENG Guo-Chu1,YUE Gao-Hong1,XU Jian-Long3,LU Hua-Jin1,*   

  1. 1 Southern Zhejiang Key Laboratory of Crop Breeding, Wenzhou Academy of Agricultural Sciences, Wenzhou 325006, China; 2 Wenzhou Plant Protection Station, Wenzhou 325000, China; 3 Institute of Crop Sciences/ National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2015-06-12 Revised:2015-09-06 Published:2016-01-12 Published online:2015-10-13
  • Contact: 卢华金, E-mail: luhjzw@163.com
  • Supported by:

    This research was supported by Special Projects of the Zhejiang Agricultural Key Research (8812 Plan, 2012C12901-17) and Wenzhou Seeds and Seedlings Technology Innovation (N20120019)), Project of the Zhejiang Education Science and Planning (2015SCG183) and General Research Program for the Education Department of Zhejiang Province (Y201533916).

摘要:

结合分子标记辅助轮回选择和田间鉴定的方法, 将三黄占2号的抗稻瘟病基因Pi-GD-1(t)Pi-GD-2(t)(分别简称G1G2)CBB23中的抗白叶枯病基因Xa23 (简称X)IR65482-7-216-1-2-B(简称IR65482)的抗褐飞虱基因Bph18(t) (简称B)导入温恢845、温恢117和温恢1433个中籼恢复系,获得了8个兼抗稻瘟病和褐飞虱聚合系,温恢845-G1-G2-B-4、温恢845-G1-G2-B-5、温恢117-G1-G2-X-B-3、温恢143-G1-G2-B-3、温恢143-G2-X-B-9、温恢143-G2-X-B-10、温恢143-G1-G2-B-11和温恢143-G1-G2-B-37。这些聚合系及其与不育系五丰A的测交种,对稻瘟病和褐飞虱的抗性水平接近或略低于稻瘟病抗性亲本三黄占2号和稻飞虱抗性亲本IR65482。部分改良恢复系如温恢117-G1-G2-X-B-3、温恢143-G2-X-B-9和温恢143-G2-X-B-10及其测交种对白叶枯病表现为抗病或中抗。改良恢复系及其测交种在正常条件下的农艺性状与原始恢复系及其测交种相仿或更优,具有生产应用价值。研究结果表明,Xa23在不同恢复系背景下抗性表达完全,而Pi-GD-1(t)Pi-GD-2(t)Bph18(t)对稻瘟病和褐飞虱抗性的改良效果与恢复系的遗传背景有关。

关键词: 水稻恢复系, 稻瘟病, 白叶枯病, 褐飞虱, 抗性改良, 标记辅助选择

Abstract:

The novel blast resistance genes, Pi-GD-1(t) and Pi-GD-2(t) (abbreviated as G1 and G2, respectively) from rice cultivar Sanhuangzhan2, bacterial blight resistance gene Xa23 (abbreviated as X) from CBB23 and brown planthopper resistance gene Bph18(t) (abbreviated as B) from IR65482-7-216-1-2-B (abbreviated as IR65482) were introgressed into three rice restorer lines, Wenhui 845, Wenhui 117 and Wenhui 143, by marker-assisted backcross and pyramiding breeding methods integrated with artificial inoculation, natural induction and phenotypic selections. The eight resistant restorer lines pyramiding blast and brown planthopper resistance genes, i.e. Wenhui 845-G1-G2-B-4, Wenhui 845-G1-G2-B-5, Wenhui 117-G1-G2-X-B-3, Wenhui 143-G1-G2-B-3, Wenhui 143-G2-X-B-9, Wenhui 143-G2-X-B-10, Wenhui 143-G1-G2-B-11, and Wenhui 143-G1-G2-B-37, and their tester lines crossed with the sterile line Wufeng A demonstrated the similar or slight lower level of resistance against rice blast and brown planthopper as compared with the resistant donor parents Sanhuangzhan2 or IR65482. Part of developed restorer lines (Wenhui 117-G1-G2-X-B-3, Wenhui 143-G2-X-B-9 and Wenhui 143-G2-X-B-10) and their tester lines showed resistance or moderate resistance to bacterial blight. The newly developed restorers resistant to blast, bacterial blight and brown planthopper were similar or superior to their respective original types in agronomic traits under normal condition, implying these resistant restorer lines can be useful in hybrid rice breeding and production. The results indicated that Xa23 could completely express its dominant resistance against different restorer genetic backgrounds whereas effects of resistance improvement in Pi-GD-1(t), Pi-GD-2(t), and Bph18(t) depend on their genetic background.

Key words: Rice restorer lines, Blast, Bacterial blight, Brown planthopper, Resistance improvement, Marker-assisted selection

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