作物学报 ›› 2016, Vol. 42 ›› Issue (10): 1419-1428.doi: 10.3724/SP.J.1006.2016.01419
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
董萌1,2,高友菲2,韩天富2,东方阳1,*,蒋炳军2,*
DONG Meng1,2, GAO You-Fie2, HAN Tian-Fu2, DONG-FANG Yang1,*, and JIANG Bing-Jun2,*
摘要:
14-3-3蛋白家族在真核生物中普遍存在,可与其他蛋白相互作用,调控多种生理生化过程。MYB基因家族作为植物中最大的一类转录因子,广泛参与了植物的生长发育和代谢调控。本研究通过分析1个从自贡冬豆中克隆的MYB转录因子GmMYB173的亚细胞定位情况,发现GmMYB173在细胞核中特异表达;序列分析发现GmMYB173与GmMYB176相似,具有1个14-3-3蛋白的潜在结合结构域,即pST结合结构域。通过重叠延伸PCR (SOE-PCR)删除了GmMYB173序列中pST结合结构域编码序列,发现GmMYB173细胞核表达特异性消失。酵母双杂交互作分析表明,大豆基因组中所有具有表达的16个14-3-3蛋白GmSGF14a~GmSGF14p均能与GmMYB173互作。β-半乳糖苷酶活性分析发现,与GmMYB173互作最强的是GmSGF14n,GmSGF14k、GmSGF14e和GmSGF14o其次。这些结果说明14-3-3蛋白不仅与GmMYB173互作,且可能调控其在细胞内的定位,有助于研究14-3-3蛋白与GmMYB173的互作关系及其在大豆生长发育中的作用。
[1]BrandtJ,Thordal-ChristensenH,VadK,GregersenPL,CollungeDB.Apathogen-inducedgeneofbarleyencodesaproteinshowinghighsimilaritytoaproteinkinaseregulator.PlantJ,1992,2:815–820 [2]LuG,DeLisleAJ,deVettenNC,FerlRJ.Brainproteinsinplants:anArabidopsishomologtoneurotransmitterpathwayactivatorsispartofaDNAbindingcomplex.ProcNatlAcadSciUSA,1992,89:11490–11494 [3]HirschS,AitkenA,BertschU,SollJ.Aplanthomologuetomammalianbrain14-3-3proteinandproteinkinaseCinhibitor.FEBSLett,1992,296:222–224 [4]DeLilleJM,SehnkePC,FerlRJ.TheArabidopsis14-3-3familyofsignalingregulators.PlantPhysiol,2001,126:35–38 [5]RosenquistM,AlsterfjordM,LarssonC,SommarinM.DataminingtheArabidopsisgenomerevealsfifteen14-3-3genes.Expressionisdemonstratedfortwooutoffivenovelgenes.PlantPhysiol,2001,127:142–149 [6]YaoY,DuY,JiangL,LiuJY.Molecularanalysisandexpressionpatternsofthe14-3-3genefamilyfromOryzasativa.JBiochemMolBiol,2007,40:349–357 [7]LiX,DhaubhadelS.Soybean14-3-3genefamily:identificationandmolecularcharacterization.Planta,2011,233:569–582 [8]YaffeMB,RittingerK,VoliniaS,CaronPR,AitkenA,LeffersH,GamblinSJ,SmerdonSJ,CantleyLC.Thestructuralbasisfor14-3-3:phosphopeptidebindingspecificity.Cell,1997,91:961–971 [9]FuH,SubramanianRR,MastersSC.14-3-3proteins:structure,function,andregulation.AnnuRevPharmacolToxicol,2000,40:617–647 [10]SumiokaA,NagaishiS,YoshidaT,LinA,MiuraM,SuzukiT.Roleof14-3-3γinFE65-dependentgenetransactivationmediatedbytheamyloidβ-proteinprecursor.JBiolChem,2005,280:42364–42374 [11]CasarettoJ,HoTH.ThetranscriptionfactorsHvABI5andHvVP1arerequiredfortheabscisicacidinductionofgeneexpressioninbarleyaleuronecells.PlantCell,2003,15:271–284 [12]FoltaKM,PaulAL,MayfieldJD,FerlRJ.14-3-3isoformsparticipateinredlightsignalingandphotoperiodicflowering.PlantSignalBehav,2008,3:304–306 [13]HuberSC,MacKintoshC,KaiserWM.Metabolicenzymesastargetsfor14-3-3proteins.PlantMolBio,2002,50:1053–1063 [14]PaulAL,FoltaKM,FerlRJ.14-3-3proteins,redlightandphotoperiodicflowering:apointofconnection.PlantSignalBehav,2008,3:511–515 [15]HajduchM,GanapathyA,SteinJW,ThelenJJ.Asystematicproteomicstudyofseedfillinginsoybean.Establishmentofhigh-resolutiontwo-dimensionalreferencemaps,expressionprofiles,andaninteractiveproteomedatabase.PlantPhysiol,2005,137:1397–1419 [16]ProuseMB,CampbellMM.TheinteractionbetweenMYBproteinsandtheirtargetDNAbindingsites.BiochimBiophysActa,2012,1819:67–77 [17]陈清,汤浩茹,董晓莉,侯艳霞,罗娅,蒋艳,黄琼瑶.植物Myb转录因子的研究进展.基因组学与应用生物学,2009,28:365–372 ChenQ,TangHR,DongXL,HouYX,LuoY,JiangY,HuangQY.ProgressintheStudyofPlantMybTranscriptionFactors.GenoApplBiol,2009,28:365–372(inChinesewithEnglishabstract) [18]LiaoY,ZouHF,WangHW,ZhangWK,MaB,ZhangJS,ChenSY.SoybeanGmMYB76,GmMYB92,andGmMYB177genesconferstresstoleranceintransgenicArabidopsisplants.CellRes,2008,18:1047–1060 [19]李晓薇.大豆两个MYB转录因子基因的克隆及其功能分析.吉林大学博士学位论文,吉林长春,2011 LiXW.CloningandCharacterizationofTwoMYBTranscriptionFactorGenesfromSoybean.PhDDissertationofJilinUniversity,Changchun,China,2011(inChinesewithEnglishabstract) [20]LiuYF,LiQT,LuX,SongQX,LamSM,ZhangWK,MaB,LinQ,ManWQ,DuWG,ShuiGH,ChenSY,ZhangJS.SoybeanGmMYB73promoteslipidaccumulationintransgenicplants.BMCPlantBiol,2014,14:1 [21]ChenL,BernhardtA,LeeJ,HellmannH.IdentificationofArabidopsisMYB56asaNovelSubstrateforCRL3(BPM)E3Ligases.MolPlant,2015,8:242–250 [22]LiuL,ZhangJ,AdrianJ,GissotL,CouplandG,YuD,TurckF.ElevatedLevelsofMYB30inthephloemacceleratefloweringinArabidopsisthroughtheregulationofFLOWERINGLOCUST.PloSOne,2014,9:e89799 [23]YiJ,DerynckMR,LiX,TelmerP,MarsolaisF,DhaubhadelS.Asingle-repeatMYBtranscriptionfactor,GmMYB176,regulatesCHS8geneexpressionandaffectsisoflavonoidbiosynthesisinsoybean.PlantJ,2010,62:1019–1034 [24]LiX,ChenL,DhaubhadelS.14-3-3proteinsregulatetheintracellularlocalizationofthetranscriptionalactivatorGmMYB176andaffectisoflavonoidsynthesisinsoybean.PlantJ,2012,71:239–250 [25]高友菲,岳岩磊,蒋炳军,韩天富.大豆GmFT2a启动子InDel区结合蛋白的筛选.中国油料作物学报,2015,37:27–34 GaoYF,YueYL,JiangBJ,HanTF.ScreeningofproteinbindingtoGmFT2apromoterindelregioninsoybean.ChinJOilCropSci,2015,37:27–34(inChinesewithEnglishabstract) [26]曲梦楠.大豆GmFT2b基因的功能分析.中国农业科学院硕士学位论文,北京,2014 QuMN.FunctionAnalysisofGmFT2bGeneinGlyminemax.MSThesisofChineseAcademyofAgriculturalSciences,Beijing,China,2014(inChinesewithEnglishabstract) [27]邢浩然,刘丽娟,刘国振.植物蛋白质的亚细胞定位研究进展.华北农学报,2006,21(增刊2):1–6 XingHR,LiuLJ,LiuGZ.AdvancementofProteinSubcellularLocalizationinPlant.ActaAgricBoreali-Sin,2006,21(S2):1–6(inChinesewithEnglishabstract) [28]刘海燕,冯冬茹,刘兵,何炎明,王宏斌,王金发.农杆菌介导的MpASR蛋白在洋葱表皮细胞的定位研究.热带亚热带植物学报,2009,17:218–222 LiuHY,FengDR,LiuB,HeYM,WangHB,WangJF.StudiesonsubcellularlocalizationofMpASRinonionepidermalcellsmediatedbyAgrobacterium.JTrop&SubtropBot,2009,17:218–222(inChinesewithEnglishabstract) [29]JinH,XuG,MengQ,HuangF,YuD.GmNAC5,aNACtranscriptionfactor,isatransientresponseregulatorinducedbyabioticstressinsoybean.SciWorldJ,2013,2013:768–972 [30]NanH,CaoD,ZhangD,LiY,LuS,TangL,YuanX,LiuB,KongF.GmFT2aandGmFT5aredundantlyanddifferentiallyregulatefloweringthroughinteractionwithandupregulationofthebZIPtranscriptionfactorGmFDL19insoybean.PloSOne,2014,9:e97669 |
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