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作物学报 ›› 2017, Vol. 43 ›› Issue (02): 210-217.doi: 10.3724/SP.J.1006.2017.00210

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于数字基因表达谱分析的茶树花不育基因挖掘

陈林波1,**   夏丽飞1,**   田易萍1   李 梅1   宋维希1   梁名志1,*#br# 江昌俊2,*   

  1. 1云南省农业科学院茶叶研究所 / 云南省茶树种质资源创新与配套栽培技术工程研究中心, 云南勐海 666201; 2安徽农业大学茶树生物学与资源利用国家重点实验室, 安徽合肥 230036
  • 收稿日期:2016-04-17 修回日期:2016-09-18 出版日期:2017-02-12 网络出版日期:2016-09-30
  • 通讯作者: 梁名志, E-mail: liangmingzhi@26.com; 江昌俊, E-mail: jiangcj@ahau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31560220, 31460216)，云南省人才培养计划项目(2015HB105)，茶树生物学与资源利用国家重点实验室开放基金项目(SKLTOF20150105)资助。

Exploring Sterility Gene from Tea Plant Flower Based on Digital Gene Expression Profiling

CHEN Lin-Bo1,**, XIA Li-Fei1,**, TIAN Yi-Ping1, LI Mei1, SONG Wei-Xi1, LIANG Ming-Zhi1,*, and JIANG Chang-Jun2,*   

  1. 1 Tea Research Institute，Yunnan Academy of Agricultural Sciences / Yunnan Engineering Research Center of Tea Germplasm Innovation and Matching Cultivation, Menghai 666201, China; 2 State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China
  • Received:2016-04-17 Revised:2016-09-18 Published:2017-02-12 Published online:2016-09-30
  • Contact: 梁名志, E-mail: liangmingzhi@26.com; 江昌俊, E-mail: jiangcj@ahau.edu.cn

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摘要：

茶树是重要的叶用经济作物，但是茶树每年都要开花结实，消耗大量的养分，导致茶树鲜叶产量减少和品质降低。了解茶树的不育机制将有助于茶树不育品种的分子遗传改良。本研究以福鼎大白茶(父本)、佛香2号(母本)以及他们的杂交后代(不育)茶树花为材料，利用数字基因表达谱技术对3个茶树花的cDNA文库进行差异基因表达谱分析。筛选出在父本花与子代不育花、母本花与子代不育花共有而在父本花与母本花没有的差异表达基因1219条。GO功能显著性分析表明，代谢过程、催化活性、水解酶活性表现为富集；KEGG代谢分析表明，差异表达基因涉及氨基酸、糖、次生代谢、植物信号传导途径以及能量代谢等过程。以植物激素信号转导通路分析发现，16个与生长素信号途径相关基因中，除5个ARF家族基因在子代不育花中上调表达外，其他的基因均下调，推测生长素信号转导是茶树花不育的重要因素。随机抽取5个基因进行实时荧光定量PCR验证, 其结果与测序结果一致。表达谱分析结果将为茶树花不育机制的深入研究和不育基因的筛选奠定基础。

关键词: 茶树花, 基因表达谱, 不育基因, 差异表达基因

Abstract:

Tea tree (Camellia sinensis) blossoms every year and a lot of nutrients are consumed by itself during blooming and fruiting, resulting in decreased yield and quality of fresh tea leaf. In the present study, the flowers of Fuding Dabaicha (male parent), Foxiang 2 (female parent) and their hybrid (sterile flowers) were used to construct cDNA libraries and the gene expression profiles were analyzed by Solexa sequencing technology. A total of 1219 candidate sterility genes were identified according to their differential expression between the male/female parent and the hybrid but identical between the two parents. These sterility gene were subject to GO analysis, and metabolic process, hydrolase activity and catalytic activity were enriched. KEGG pathway analysis showed that the sterility genes were involved in biosynthesis of amino acids and carbohydrate, second any metabolism, plant hormone signal transduction, and energy metabolism. In the function group of plant hormone signal transduction, 16 annotated genes were involved in the auxin signal transduction pathway, including five up-regulated AFR genes in the hybrid and 11 down-regulated genes. Thus, we speculate that auxin signal transduction might regulate the flower sterility in tea tree. Five sterility genes were selected randomly for qRT-PCR assay and the result was consistent with that of Solexa sequencing. The candidate sterility genes found in this study are worthy of sterile mechanism research in tea plant and sterility gene screening.

Key words: Tea plant flowers, Gene expression profiles, Sterility gene, Differentially expressed genes

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