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作物学报 ›› 2017, Vol. 43 ›› Issue (08): 1122-1127.doi: 10.3724/SP.J.1006.2017.01122

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻颖花持续开放突变体sostenuto floret opening (sfo1)的鉴定与基因定位

沈亚林,庄慧,陈欢,曾晓琴,李香凝,张君,郑昊,凌英华,李云峰*   

  1. 西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室，重庆 400716
  • 收稿日期:2016-12-01 修回日期:2017-04-20 出版日期:2017-08-12 网络出版日期:2017-04-27
  • 通讯作者: 李云峰, E-mail: liyf1980@swu.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31271304)和中央高校基本科研业务费(XDJK2016A013)资助。

Characterization and Gene Mapping of sostenuto floret opening 1 (sfo1) Mutant in Rice (Oryza sativaL.)

SHEN Ya-Lin,ZHUANG Hui,CHEN Huan,ZENG Xiao-Qin,LI Xiang-Ning,ZHANG Jun,ZHENG Hao, LING Ying-Hua,LI Yun-Feng*   

  1. Rice Research Institute, Southwest University/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Chongqing 400716, China
  • Received:2016-12-01 Revised:2017-04-20 Published:2017-08-12 Published online:2017-04-27
  • Contact: Li Yuefeng, E-mail: liyf1980@swu.edu.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31271304) and Fundamental Research Funds for the Central Universities (XDJK2016A013).

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摘要：

水稻颖花开放是其生殖发育一个关键生理过程，对受精和随后种子发育具有重要影响。本文报道了一个与水稻颖花开放相关的突变体，来源于籼稻保持系西农1B的EMS(ethyl methane sulfonate)诱变群体。该突变体表现为开颖后浆片失水萎缩过程缓慢，内外稃持续开裂不闭合，暂命名为水稻颖花持续开放sostenuto floret opening 1 (sfo1)突变体。遗传分析表明sfo1性状受一对隐性单基因控制，利用群体分离分析法(bulked segregation analysis, BSA)将SFO1基因定位在第5染色体SSR标记RM1054和IN/DEL标记ZTQ51之间，物理距离113 kb，含注释基因15个。本研究结果为SFO1基因的图位克隆和功能研究奠定了基础。

关键词: 水稻, 颖花开放, 浆片, 基因定位

Abstract:

Rice floret opening is one of the most critical physiological processes in its reproductive development, which has a significant influence on the fertilization and subsequent seed development. Rice floret opening and closing are promoted respectively by the lodicules’ expansion and shrink. In recent years, many studies focused on the molecular mechanism of rice lodiculedevelopment.However, few studies focused on the molecular regulation mechanism in physiological process of lodicule opening and closing. In this paper, we reported a floret opening mutant, sostenuto floret opening 1 (sfo1), derived from EMS (ethyl methane sulfonate) mutation groups of Xinong 1B.During the floret opening stage, the florets of sfo1 and wild type were observed directly by both the stereoscope and the scanning electron microscopy at about 1 h before opening, and 10 min, 1.5 h and 48 h after openingrespecting. Compared withthat in the wild type, the sfo1floret showed a delay of lodicules dehydration after floret opening, resulting in its failing to close the lemma and palea. Significant differenceswere not found at1h before opening, showing closed hulls, flat lodicules and well-stackedlodicules surface cells.At 10 min after opening, there were also no obvious differences between wild type and mutants,showing openinghulls, inflated lodicules, and smooth and well-stacked surface cells. At1.5 h after opening, sfo1 hulls were not closed so big as those in the wild type. And sfo1lodicules still inflated, but not dehydrated and atrophied as those in thewild type. At 48 h after opening, sfo1hullswere not closed yet, its lodiculesbegan to dehydrate and atrophy, but were still very full compared with those in the wild type. The F1and F2 of across with sterile lines 56S as female parent and the sfo1 as male parent wereused in genetic analysis and gene mapping, indicating that the sfo1 trait was controlled by a nuclear recessive gene. Using bulked segregation analysis (BSA) method, the SFO1 was located between the SSR marker RM1054 and Insert/Delete marker ZTQ51 on the chromosome 5, with a physical distance of 113kb and including 15 candidate genes in this region. LOC_Os05g50890 and LOC_Os05g50900 were preliminary selected as candidate genes for SFO1. These results laid a foundation for the SFO1 gene cloning and functional research.

Key words: Rice (oryza sativa), floret opening, Lodicule, Gene mapping

[1]曾晓春, 周燮, 吴晓玉. 水稻颖花开放机理研究进展. 中国农业科学, 2004, 37: 188–195
Zeng X C, Zhou X, Wu X Y. Advances in study of opening mechanism in rice florets. Sci Agric Sin, 2004, 37:188–195 (in Chinese with English abstract)
[2]许祥明, 王忠, 顾蕴洁, 高珠. 某些酸类物质对水稻开颖的效应. 植物生理学报, 1998, 24: 124–130
Xu X M, Wang Z, Gu W J, Gao Z. Effects of some acids on rice glume opening. Plant Physiol J, 1998, 24: 124–130 (in Chinese with English abstract)
[3]Yadav S R, Prasad K, Vijayraghavan U. Divergent regulatory OsMADS2 functions control size, shape and differentiation ofthe highly derived rice floret second-whorl organ. Genetics, 2007,176: 283–294
[4]Yao S G, Ohmori S, Kimizu M, Yoshida H. Unequal genetic redundancy of rice PISTILLATA orthologs, OsMADS2 andOsMADS4, in lodicule and stamen development. Plant Cell Physiol, 2008, 49: 853–857
[5]Xiao H, Wang Y, Liu D F, Wang W M, Li X B, Zhao X F, Xu J C, Zhai W X, Zhu L H. Functional analysis of the rice AP3homologue OsMADS16 by RNA interference. Plant Mol Biol, 2003, 52: 957–966
[6]Li H F, Liang W Q, Jia R D, Yin C S, Zong J, Kong H Z, Zhang D B. The AGL6-like gene OsMADS6 regulates floral organand meristem identities in rice. Cell Res, 2010, 20: 299–313
[7]Nam J, Kim J, Lee S, An G, Ma H, Nie M. Type I MADS-box genes have experienced faster birth-and-death evolution than type II MADS-box genes in angiosperms. Proc Natl Acad SciUSA, 2004, 101: 1910–1915
[8]Xiao H, Tang J F, Li Y F, Wang W M, Li X B, Jin L, Xie R, Luo H F, Zhao X F, Meng Z. STAMENLESS1, encoding asingle C2H2 zinc finger protein, regulates floral organ identity in rice. Plant J, 2009, 5: 789–801
[9]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.Proc Natl Acad SciUSA, 1991, 88: 9828–9832
[10]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 1980, 8: 4321–4325
[11]Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene RL10(t) in rice (Oryza sativa L.). Genome, 2007, 50: 811–817
[12]Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353: 31–37
[13]Weigel D, Meyerowitz E M. The ABCs of floral homeotic genes. Cell, 1994, 78: 203–209
[14]Theissen G, Saedler H. Plant biology: floral quartets. Nature, 2001, 409: 469–471
[15]Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, NagatoY. SUPERWOMAN1andDROOPING LEAF genescontrol floral organ identity in rice. Development, 2003, 130: 705−718
[16]Yoshida H, Itoh J I, Ohmori S, Miyoshi K, Horigome A, Uchida E, Kimizu M, Matsumura Y, Kusaba M, Satoh H, Nagato Y. Superwoman1-cleistogamy, a hopeful allele for gene containment in GM rice. Plant Biotechnol J, 2007, 5: 835–846
[17]黄俊宝, 何永明, 曾晓春, 向妙莲, 付永琦. 水稻颖花开放前花器官茉莉酸水平变化及浆片茉莉酸信号基因表达分析. 中国农业科学, 2015, 48: 1219–1227
Huang J B, He Y M, Zeng X C, Xiang M L, Fu Y Q. Changes of JA levels in floral organs and expression analysis of JA signaling genes in lodicules before floret opening in rice. Sci Agric Sin, 2015, 48: 1219–1227 (in Chinese with English abstract)
[18]Ohashi-Ito K, Bergmann D C. Arabidopsis FAMA controls the final proliferation/differentiation switch during stomatal development. Plant Cell, 2006, 18: 2493–2505
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