欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2017, Vol. 43 ›› Issue (08): 1122-1127.doi: 10.3724/SP.J.1006.2017.01122

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻颖花持续开放突变体sostenuto floret opening (sfo1)的鉴定与基因定位

沈亚林,庄慧,陈欢,曾晓琴,李香凝,张君,郑昊,凌英华,李云峰*   

  1. 西南大学水稻研究所/转基因植物与安全控制重庆市重点实验室，重庆 400716
  • 收稿日期:2016-12-01 修回日期:2017-04-20 出版日期:2017-08-12 网络出版日期:2017-04-27
  • 通讯作者: 李云峰, E-mail: liyf1980@swu.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(31271304)和中央高校基本科研业务费(XDJK2016A013)资助。

Characterization and Gene Mapping of sostenuto floret opening 1 (sfo1) Mutant in Rice (Oryza sativaL.)

SHEN Ya-Lin,ZHUANG Hui,CHEN Huan,ZENG Xiao-Qin,LI Xiang-Ning,ZHANG Jun,ZHENG Hao, LING Ying-Hua,LI Yun-Feng*   

  1. Rice Research Institute, Southwest University/Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Chongqing 400716, China
  • Received:2016-12-01 Revised:2017-04-20 Published:2017-08-12 Published online:2017-04-27
  • Contact: Li Yuefeng, E-mail: liyf1980@swu.edu.cn
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31271304) and Fundamental Research Funds for the Central Universities (XDJK2016A013).

RichHTML

PDF

859

摘要：

水稻颖花开放是其生殖发育一个关键生理过程，对受精和随后种子发育具有重要影响。本文报道了一个与水稻颖花开放相关的突变体，来源于籼稻保持系西农1B的EMS(ethyl methane sulfonate)诱变群体。该突变体表现为开颖后浆片失水萎缩过程缓慢，内外稃持续开裂不闭合，暂命名为水稻颖花持续开放sostenuto floret opening 1 (sfo1)突变体。遗传分析表明sfo1性状受一对隐性单基因控制，利用群体分离分析法(bulked segregation analysis, BSA)将SFO1基因定位在第5染色体SSR标记RM1054和IN/DEL标记ZTQ51之间，物理距离113 kb，含注释基因15个。本研究结果为SFO1基因的图位克隆和功能研究奠定了基础。

关键词: 水稻, 颖花开放, 浆片, 基因定位

Abstract:

Rice floret opening is one of the most critical physiological processes in its reproductive development, which has a significant influence on the fertilization and subsequent seed development. Rice floret opening and closing are promoted respectively by the lodicules’ expansion and shrink. In recent years, many studies focused on the molecular mechanism of rice lodiculedevelopment.However, few studies focused on the molecular regulation mechanism in physiological process of lodicule opening and closing. In this paper, we reported a floret opening mutant, sostenuto floret opening 1 (sfo1), derived from EMS (ethyl methane sulfonate) mutation groups of Xinong 1B.During the floret opening stage, the florets of sfo1 and wild type were observed directly by both the stereoscope and the scanning electron microscopy at about 1 h before opening, and 10 min, 1.5 h and 48 h after openingrespecting. Compared withthat in the wild type, the sfo1floret showed a delay of lodicules dehydration after floret opening, resulting in its failing to close the lemma and palea. Significant differenceswere not found at1h before opening, showing closed hulls, flat lodicules and well-stackedlodicules surface cells.At 10 min after opening, there were also no obvious differences between wild type and mutants,showing openinghulls, inflated lodicules, and smooth and well-stacked surface cells. At1.5 h after opening, sfo1 hulls were not closed so big as those in the wild type. And sfo1lodicules still inflated, but not dehydrated and atrophied as those in thewild type. At 48 h after opening, sfo1hullswere not closed yet, its lodiculesbegan to dehydrate and atrophy, but were still very full compared with those in the wild type. The F1and F2 of across with sterile lines 56S as female parent and the sfo1 as male parent wereused in genetic analysis and gene mapping, indicating that the sfo1 trait was controlled by a nuclear recessive gene. Using bulked segregation analysis (BSA) method, the SFO1 was located between the SSR marker RM1054 and Insert/Delete marker ZTQ51 on the chromosome 5, with a physical distance of 113kb and including 15 candidate genes in this region. LOC_Os05g50890 and LOC_Os05g50900 were preliminary selected as candidate genes for SFO1. These results laid a foundation for the SFO1 gene cloning and functional research.

Key words: Rice (oryza sativa), floret opening, Lodicule, Gene mapping

[1]曾晓春, 周燮, 吴晓玉. 水稻颖花开放机理研究进展. 中国农业科学, 2004, 37: 188–195
Zeng X C, Zhou X, Wu X Y. Advances in study of opening mechanism in rice florets. Sci Agric Sin, 2004, 37:188–195 (in Chinese with English abstract)
[2]许祥明, 王忠, 顾蕴洁, 高珠. 某些酸类物质对水稻开颖的效应. 植物生理学报, 1998, 24: 124–130
Xu X M, Wang Z, Gu W J, Gao Z. Effects of some acids on rice glume opening. Plant Physiol J, 1998, 24: 124–130 (in Chinese with English abstract)
[3]Yadav S R, Prasad K, Vijayraghavan U. Divergent regulatory OsMADS2 functions control size, shape and differentiation ofthe highly derived rice floret second-whorl organ. Genetics, 2007,176: 283–294
[4]Yao S G, Ohmori S, Kimizu M, Yoshida H. Unequal genetic redundancy of rice PISTILLATA orthologs, OsMADS2 andOsMADS4, in lodicule and stamen development. Plant Cell Physiol, 2008, 49: 853–857
[5]Xiao H, Wang Y, Liu D F, Wang W M, Li X B, Zhao X F, Xu J C, Zhai W X, Zhu L H. Functional analysis of the rice AP3homologue OsMADS16 by RNA interference. Plant Mol Biol, 2003, 52: 957–966
[6]Li H F, Liang W Q, Jia R D, Yin C S, Zong J, Kong H Z, Zhang D B. The AGL6-like gene OsMADS6 regulates floral organand meristem identities in rice. Cell Res, 2010, 20: 299–313
[7]Nam J, Kim J, Lee S, An G, Ma H, Nie M. Type I MADS-box genes have experienced faster birth-and-death evolution than type II MADS-box genes in angiosperms. Proc Natl Acad SciUSA, 2004, 101: 1910–1915
[8]Xiao H, Tang J F, Li Y F, Wang W M, Li X B, Jin L, Xie R, Luo H F, Zhao X F, Meng Z. STAMENLESS1, encoding asingle C2H2 zinc finger protein, regulates floral organ identity in rice. Plant J, 2009, 5: 789–801
[9]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations.Proc Natl Acad SciUSA, 1991, 88: 9828–9832
[10]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res, 1980, 8: 4321–4325
[11]Luo Z K, Yang Z L, Zhong B Q, Li Y F, Xie R, Zhao F M, Ling Y H, He G H. Genetic analysis and fine mapping of a dynamic rolled leaf gene RL10(t) in rice (Oryza sativa L.). Genome, 2007, 50: 811–817
[12]Coen E S, Meyerowitz E M. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353: 31–37
[13]Weigel D, Meyerowitz E M. The ABCs of floral homeotic genes. Cell, 1994, 78: 203–209
[14]Theissen G, Saedler H. Plant biology: floral quartets. Nature, 2001, 409: 469–471
[15]Nagasawa N, Miyoshi M, Sano Y, Satoh H, Hirano H, Sakai H, NagatoY. SUPERWOMAN1andDROOPING LEAF genescontrol floral organ identity in rice. Development, 2003, 130: 705−718
[16]Yoshida H, Itoh J I, Ohmori S, Miyoshi K, Horigome A, Uchida E, Kimizu M, Matsumura Y, Kusaba M, Satoh H, Nagato Y. Superwoman1-cleistogamy, a hopeful allele for gene containment in GM rice. Plant Biotechnol J, 2007, 5: 835–846
[17]黄俊宝, 何永明, 曾晓春, 向妙莲, 付永琦. 水稻颖花开放前花器官茉莉酸水平变化及浆片茉莉酸信号基因表达分析. 中国农业科学, 2015, 48: 1219–1227
Huang J B, He Y M, Zeng X C, Xiang M L, Fu Y Q. Changes of JA levels in floral organs and expression analysis of JA signaling genes in lodicules before floret opening in rice. Sci Agric Sin, 2015, 48: 1219–1227 (in Chinese with English abstract)
[18]Ohashi-Ito K, Bergmann D C. Arabidopsis FAMA controls the final proliferation/differentiation switch during stomatal development. Plant Cell, 2006, 18: 2493–2505
[1] 田甜, 陈丽娟, 何华勤. 基于Meta-QTL和RNA-seq的整合分析挖掘水稻抗稻瘟病候选基因[J]. 作物学报, 2022, 48(6): 1372-1388.
[2] 郑崇珂, 周冠华, 牛淑琳, 和亚男, 孙伟, 谢先芝. 水稻早衰突变体esl-H5的表型鉴定与基因定位[J]. 作物学报, 2022, 48(6): 1389-1400.
[3] 周文期, 强晓霞, 王森, 江静雯, 卫万荣. 水稻OsLPL2/PIR基因抗旱耐盐机制研究[J]. 作物学报, 2022, 48(6): 1401-1415.
[4] 郑小龙, 周菁清, 白杨, 邵雅芳, 章林平, 胡培松, 魏祥进. 粳稻不同穗部籽粒的淀粉与垩白品质差异及分子机制[J]. 作物学报, 2022, 48(6): 1425-1436.
[5] 颜佳倩, 顾逸彪, 薛张逸, 周天阳, 葛芊芊, 张耗, 刘立军, 王志琴, 顾骏飞, 杨建昌, 周振玲, 徐大勇. 耐盐性不同水稻品种对盐胁迫的响应差异及其机制[J]. 作物学报, 2022, 48(6): 1463-1475.
[6] 杨建昌, 李超卿, 江贻. 稻米氨基酸含量和组分及其调控[J]. 作物学报, 2022, 48(5): 1037-1050.
[7] 杨德卫, 王勋, 郑星星, 项信权, 崔海涛, 李生平, 唐定中. OsSAMS1在水稻稻瘟病抗性中的功能研究[J]. 作物学报, 2022, 48(5): 1119-1128.
[8] 朱峥, 王田幸子, 陈悦, 刘玉晴, 燕高伟, 徐珊, 马金姣, 窦世娟, 李莉云, 刘国振. 水稻转录因子WRKY68在Xa21介导的抗白叶枯病反应中发挥正调控作用[J]. 作物学报, 2022, 48(5): 1129-1140.
[9] 王小雷, 李炜星, 欧阳林娟, 徐杰, 陈小荣, 边建民, 胡丽芳, 彭小松, 贺晓鹏, 傅军如, 周大虎, 贺浩华, 孙晓棠, 朱昌兰. 基于染色体片段置换系群体检测水稻株型性状QTL[J]. 作物学报, 2022, 48(5): 1141-1151.
[10] 王泽, 周钦阳, 刘聪, 穆悦, 郭威, 丁艳锋, 二宫正士. 基于无人机和地面图像的田间水稻冠层参数估测与评价[J]. 作物学报, 2022, 48(5): 1248-1261.
[11] 陈悦, 孙明哲, 贾博为, 冷月, 孙晓丽. 水稻AP2/ERF转录因子参与逆境胁迫应答的分子机制研究进展[J]. 作物学报, 2022, 48(4): 781-790.
[12] 刘磊, 詹为民, 丁武思, 刘通, 崔连花, 姜良良, 张艳培, 杨建平. 玉米矮化突变体gad39的遗传分析与分子鉴定[J]. 作物学报, 2022, 48(4): 886-895.
[13] 王吕, 崔月贞, 吴玉红, 郝兴顺, 张春辉, 王俊义, 刘怡欣, 李小刚, 秦宇航. 绿肥稻秆协同还田下氮肥减量的增产和培肥短期效应[J]. 作物学报, 2022, 48(4): 952-961.
[14] 巫燕飞, 胡琴, 周棋, 杜雪竹, 盛锋. 水稻延伸因子复合体家族基因鉴定及非生物胁迫诱导表达模式分析[J]. 作物学报, 2022, 48(3): 644-655.
[15] 陈云, 李思宇, 朱安, 刘昆, 张亚军, 张耗, 顾骏飞, 张伟杨, 刘立军, 杨建昌. 播种量和穗肥施氮量对优质食味直播水稻产量和品质的影响[J]. 作物学报, 2022, 48(3): 656-666.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2