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作物学报 ›› 2017, Vol. 43 ›› Issue (08): 1128-1138.doi: 10.3724/SP.J.1006.2017.01128

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米SNAC基因的遗传变异及耐旱性调控

李国君1,3,**,马艺文2,3,**,徐丹阳3,吴永波3,宋洁3,王楠3,郝转芳3,*,赵娟1,*   

  1. 1山西农业大学农学院,山西太谷030801;2吉林省通化市农业科学研究院,吉林梅河口135007;3中国农业科学院作物科学研究所,北京100081
  • 收稿日期:2016-11-29 修回日期:2017-04-20 出版日期:2017-08-12 网络出版日期:2017-05-11
  • 通讯作者: 李国君,E-mail:liguojun911@163.com,Tel:18734422015;马艺文,E-mail:mayiwen3070@163.com,Tel:13943529373
  • 基金资助:

    本研究由国家自然科学基金重大国际合作项目(31661143010)和面上项目(31271735)资助。

Genetic Variations and Drought Tolerance of SNAC Genes in Common Maize Inbred Lines of China

LIGuo-Jun1,3,**,MAYi-Wen2,3,**,XUDan-Yang3,WUYong-Bo3,SONGJie3,WANGNan3,HAOZhuan-Fang3,*,ZHAOJuan1,*   

  1. 1 College of Agronomy, Shanxi Agricultural University, Taigu 030801, China; 2 Tonghua Academy of Agricultural Sciences, Tonghua 135007, China; 3 Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-11-29 Revised:2017-04-20 Published:2017-08-12 Published online:2017-05-11
  • Contact: LI Guojun,E-mail:liguojun911@163.com,Tel:18734422015;Ma Wenyi,E-mail:mayiwen3070@163.com,Tel:13943529373
  • Supported by:

    ThisstudywassupportedbytheNationalNaturalScienceFoundationofChina(31661143010;31271735).

摘要:

以我国玉米育种中常用的16份自交系为材料,通过对SNAC(Stress-responsive NAM, ATAF1/2, CUC2)基因编码区及上游启动子区800bp核苷酸序列进行测序,检测SNAC基因在不同杂种优势类群材料中的遗传变异。在12个SNAC基因中,其中有4个基因在上游800bp区检测到遗传变异,有4个基因变异位点超过30个,多态性较高。虽然大多数SNAC基因变异以SNP(Single nucleotide polymorphism)为主,但在ZmNAC031467基因中检测到较多的插入缺失变异(In/Del),达到基因总遗传变异的63.3%。通过PLACE软件对上游启动子有变异的4个基因进行3种耐逆结合元件的预测,结果显示4个基因均含有3种耐逆结合元件,但是基因突变对启动子结合元件的影响较小。再对检测到的遗传变异进行核苷酸多态性分析和中性检验,有7个SNAC基因核苷酸多态性较高,其中ZmNAC080308基因的多态性达到0.00962,推测这些基因在遗传漂移过程中受自然选择影响较大。利用T检验初步发现ZmNAC070395ZmNAC080398基因的2个变异位点与耐旱相关性状关联,为进一步分析SNAC基因核苷酸变异与耐旱性状的关系提供一定的借鉴。

关键词: 玉米, SNAC基因, 遗传变异, 耐旱性

Abstract:

The coding regions and their upstream 800 bp promoter regions of SNAC genes (Sress-responsive NAM, ATAF1/2, CUC2) were sequenced in 16 maize inbred lines commonly used in China. Among 12 SNAC genes, genetic variations in promoter region were only identified in four SNAC genes, and more than 30 variations were identified in four SNAC genes, showing higher polymorphism in the four genes than other in SNAC genes. Although most of the SNAC genes were mainly SNP (Single nucleotide polymorphism) mutations, more insertion/deletion mutations were detected in ZmNAC031467 gene, reaching 63.3% of the total genetic variations. The PLACE software was used to predict three kinds of stress-tolerant binding elements in SNAC gene, but little effect was found to be related with the variations. Additionally,high nucleotide polymorphisms were identified in seven SNAC genes, especially with the highest π value of 0.00962 in ZmNAC030308, which suggested that they were greatly influenced by natural selection in the genetic drift. With the T-test, two mutations of ZmNAC070395 and ZmNAC080398 genes were associated with drought-tolerant traits,whichprovides references for further analysing the relationship between nucleotide variation in SNAC and drought tolerance traits.

Key words: 玉米, SNAC基因, 遗传变异, 耐旱性

[1]李月.棉花逆境胁迫应答Trihelix转录因子的鉴定及功能分析.石河子大学博士学位论文,新疆石河子,2013
LiY.TheIdentificationofTrihelixTranscriptionFactorandFunctionalAnalysisinResponsetoStressinCotton.Ph.D.DissertationofShiheziUniversity,Shihezi,China,2013(inChinesewithEnglishabstract)
[2]陈儒钢,巩振辉,逯明辉,李大伟,黄炜.植物抗逆反应中的转录因子网络研究进展.农业生物技术学报,2010,18(1):126–134
ChenRG,GongZH,LuMH,LiDW,HuangW.Researchadvancedofthetranscriptionfactorsnetworksrelatedtoplantadverseenvironmentalstress.JAgricBiotechnol,2010,18(1):126–134(inChinesewithEnglishabstract)
[3]康桂娟,曾日中,聂智毅,黎瑜,代龙军,段翠芳.植物NAC转录因子的研究进展.生物技术通报,2012,(11):21–26
KangGJ,ZengRZ,NieZY,LiY,DaiLJ,DuanCF.ResearchprogressofplantNACtranscriptionfactors.BiotechnolBull,2012,11:21–26(inChinesewithEnglishabstract)
[4]SouerE,HouwelingenVA,KloosD,MolJ,KoesR.Thenoapicalmeristemgeneofpetuniaisrequiredforpatternformationinembryosandflowersandisexpressedatmeristemandprimordialboundaries.Cell,1996,85:159–170
[5]AidaM,IshidaT,FukakiH,FujisawaH,TasakaM.GenesinvolvedinorganseparationinArabidopsis:ananalysisofthecup-shapedcotyledonmutant.PlantCell,1997,9:841–857
[6]TranLS,NakashimaK,SakumaY,SimpsonSD,FujitaY,MaruyamaK,FujitaM,SekiM,ShinozakiK,Yamaguchi-ShinozakiK.IsolationandfunctionalanalysisofArabidopsisstress-inducibleNACtranscriptionfactorsthatbindtoadrought-responsivecis-elementintheearlyresponsivetodehydrationstress1promoter.PlantCell,2004,16:2481–2498
[7]MitsudaN,HisaboriT,TakeyasuK,SatoMH.VOZ;isolationandcharacterizationofnovelvascularplanttranscriptionfactorswithaone-zincfingerfromArabidopsisthaliana.PlantCellPhysiol,2004,45:845–854
[8]FangYJ,YouJ,XieKB,XieWB,XiongLZ.Systematicsequenceanalysisandidentificationoftissue-specificorstress-responsivegenesofNACtranscriptionfactorfamilyinrice.MolGenetGenomics,2008,280:535–546
[9]ShenH,YinYB,ChenF,XuY,DixonRA.AbioinformaticanalysisofNACgenesforplantcellwalldevelopmentinrelationtolignocellulosicbioenergyproduction.BioEnergyRes,2009,2:217–232
[10]NuruzzamanM,ManimekalaiR,SharoniAM,SatohK,KondohH,OokaH,KikuchiS.Genome-wideanalysisofNACtranscriptionfactorfamilyinrice.Gene,2010,465:30–44
[11]NakashimaK,TakasakiH,MizoiJ,ShinozakiK,Yamaguchi-ShinozakiK.NACtranscriptionfactorsinplantabioticstressresponses.BiochimBiophysActa,2012,1819:97–103
[12]RiechmannJL,HeardJ,MartinG,ReuberL,JiangC,KeddieJ,AdamL,PinedaO,RatcliffeOJ,SamahaRR,CreelmanR,PilgrimM,BrounP,ZhangJZ,GhandehariD,ShermanBK,YuG.Arabidopsistranscriptionfactors:genome-widecomparativeanalysisamongeukaryotes.Science,2000,290:2105–2110
[13]LuM,YingS,ZhangDF,ShiYS,SongYC,WangTY,LiY.Amaizestress-responsiveNACtranscriptionfactor,ZmSNAC1,confersenhancedtolerancetodehydrationintransgenicArabidopsis.PlantCellRep,2012,31:1701–1711
[14]MaoHD,WangHW,LiuSX,LiZG,YangXH,YanJB,LiJS,PhanTranLS,QinF.AtransposableelementinaNACgeneisassociatedwithdroughttoleranceinmaizeseedlings.NatCommun,2015,6:8326
[15]LiuSS,HaoZF,WengJF,LiMS,ZhangDG,PanTG,ZhangSH,LiXH.Identificationoftwofunctionalmarkersassociatedwithdroughtresistanceinmaize.MolBreed,2015,35:1–10
[16]HaoZF,LiXH,SuZJ,XieCX,LiMS,LiangXL,WengJF,ZhangDG,LiL,ZhangSH.Aproposedselectioncriterionfordroughtresistanceacrossmultipleenvironmentsinmaize.BreedSci,2011,61:101–108
[17]张世煌.商业育种只需要两个杂种优势群.种子科技,2014,(7):7–8
ZhangSH.Onlyneedtwoheteroticgroupsincommercialbreeding.SeedScience&Technology,2014,(7):7–8(inChinese)
[18]MurrayMG,ThompsonWF.RapidisolationofhighmolecularweightplantDNA.NuclAcidsRes,1980,8:4321–4326
[19]LiL,MaYW,ZhangSH,HaoZF,LiXH.ZeamaysNACtranscriptionfactorfamilymembers:theirgenomiccharacteristicsandrelationshipwithdroughtstress.ResJBiotechnol,2015,1:63–77
[20]ZhaoY,ZhouYQ,JiangHY,LiXY,GanDF,PengXJ,ZhuSW,ChengBJ.Systematicanalysisofsequencesandexpressionpatternsofdrought-responsivemembersoftheHD-Zipgenefamilyinmaize.PLoSOne,2011,6:e28488
[21]NeiM,LiWH.Mathematicalmodelforstudyinggeneticvariationintermsofrestrictionendonucleases.ProcNatlAcadSciUSA,1979,10:5269–5273
[22]周琦,王文.DNA水平自然选择作用的检测.动物学研究,2004,25(1):73–80
ZhouQ,WangW.DetectingnaturalselectionattheDNAlevel.ZoolRes,2004,25(1):73–80(inChinesewithEnglishabstract)
[23]李伟,韩蕾,钱永强,孙振元.植物NAC转录因子的种类、特征及功能.应用与环境生物学报,2011,17:596–606
LiW,HanL,QianYQ,SunZY.CharacteristicsandfunctionsofNACtranscriptionfactorsinplants.ChinJApplEnvironBiol,2011,17:596–606(inChinesewithEnglishabstract)
[24]HuHH,DaiMQ,YaoJL,XiaoBZ,LiXH,ZhangQF,XiongLZ.OverexpressingaNAM,ATAF,andCUC(NAC)transcriptionfactorenhancesdroughtresistanceandsalttoleranceinrice.ProcNatlAcadSciUSA,2006,103:12987–12992
[25]KanedaT,TagaY,TakaiR,IwanoM,MatsuiH,TakayamaS,IsogaiA,CheF.ThetranscriptionfactorOsNAC4isakeypositiveregulatorofplanthypersensitivecelldeath.EMBOJ,2009,28:926–936
[26]NakayamaA,FukushimaS,GotoS,MatsushitaA,ShimonoM,SuganoS,JiangCJ,AkagiA,YamazakiM,InoueM,TakatsujiH.Genome-wideidentificationofWRKY45-regulatedgenesthatmediatebenzothiadiazole-induceddefenseresponsesinrice.BMCPlantBiol,2013,13:1–11
[27]SongSY,ChenY,ChenJ,DaiXY,ZhangWH.PhysiologicalmechanismsunderlyingOsNAC5-dependenttoleranceofriceplantstoabioticstress.Planta,2011,234:331–345
[28]OhnishiT,SugaharaS,YamadaT,KikuchiK,YoshibaY,HiranoHY,TsutsumiN.OsNAC6,amemberoftheNACgenefamily,isinducedbyvariousstressesinrice.GenesGenetSyst,2005,80:135–139
[29]HuHH,YouJ,FangYJ,ZhuXY,QiZY,XiongLZ.CharacterizationoftranscriotionfactorgeneSNAC2conferringcoldandsalttoleranceinrice.PlantMolBiol,2008,67:169–18
[30]FujitaM,FujitaY,MaruyamaK,SekiM,HiratsuK,Ohme-TakagiM,TranLS,Yamaguchi-ShinozakiK,ShinozakiK.Adehydration-inducedNACprotein,RD26,isinvolvedinanovelABA-dependentstress-signalingpathway.PlantJ,2004,39,863–876
[31]LuPL,ChenNZ,AnR,SuZ,QiBS,RenF,ChenJ,WangXC.Anoveldrought-induciblegene,ATAF1,encodesaNACfamilyproteinthatnegativelyregulatestheexpressionofstress-responsivegenesinArabidopsis.PlantMolBiol,2007,63:289–305
[32]WuJ,WangLF,WangSM.Comprehensiveanalysisanddiscoveryofdrought-relatedNACtranscriptionfactorsincommonbean.BMCPlantBiol,2016,16:193
[33]SekiM,NarusakaM,AbeH,KasugaM,Yamaguchi-ShinozakiK,CarninciP,HayashizakiY,ShinozakiK.Monitoringtheexpressionpatternof1300Arabidopsisgenesunderdroughtandcoldstressesbyusingafull-lengthcDNAmicroarray.PlantCell,2001,13:61–72
[34]FowlerS,ThomashowMF.ArabidopsistranscriptomeprofilingindicatesthatmultipleregulatorypathwaysareactivatedduringcoldacclimationinadditiontotheCBFcoldresponsepathway.PlantCell,2002,14:1675–1690
[35]MaruyamaK,SakumaY,KasugaM,ItoY,SekiM,GodaH,ShimadaY,YoshidaS,ShinozakiK,Yamaguchi-ShinozakiK.Identificationofcold-inducibledownstreamgenesoftheArabidopsisDREB1A/CBF3transcriptionalfactorusingtwomicroarraysystems.PlantJ,2004,38:982–993
[36]ShinozakiK,Yamaguchi-ShinozakiK.Genenetworksinvolvedindroughtstressresponseandtolerance.JExpBot,2007,58:221–227
[37]ChoiH,HongJ,HaJ,KangJ,KimSY.ABFs,afamilyofABA-responsiveelementbindingfactors.JBiolChem,2000,275:1723–1730
[38]UnoY,FurihataT,AbeH,YoshidaR,ShinozakiK,Yamaguchi-ShinozakiK.Arabidopsisbasicleucinezippertranscriptionfactorsinvolvedinanabscisicacid-dependentsignaltransductionpathwayunderdroughtandhigh-salinityconditions.ProcNatlAcadSciUSA,2000,97:11632–11637
[39]NakashimaK,TranLS,VanNguyenD,FujitaM,MaruyamaK,TodakaD,ItoY,HayashiN,ShinozakiK,Yamaguchi-ShinozakiK.FunctionalanalysisofaNAC-typetranscriptionfactorOsNAC6involvedinabioticandbioticstress-responsivegeneexpressioninrice.PlantJ,2007,51:617–630
[40]SakumaY,LiuQ,DubouzetJG,AbeH,ShinozakiK,Yamaguchi-ShinozakiK.DNA-bindingspecificityoftheERF/AP2domainofArabidopsisDREBs,transcriptionfactorsinvolvedindehydration-andcold-induciblegeneexpression.BiochemBiophysResCommun,2002,290:998–1009
[41]赵洪阳.水稻抗旱基因分子进化研究.华中农业大学硕士学位论文,湖北武汉,2012
ZhaoHY.MolecularEvolutionofDrought-ResistantGenesinRice.MSThesisofHuazhongAgriculturalUniversity,Wuhan,China,2012(inChinesewithEnglishabstract)
[42]HickmanR,HillC,PenfoldCA,BreezeE,BowdenL,MooreJD,ZhangP,JacksonA,CookeE,Bewicke-CopleyF,MeadA,BeynonJ,WildDL,DenbyKJ,OttS,Buchanan-WollastonV.AlocalregulatorynetworkaroundthreeNACtranscriptionfactorsinstressresponsesandsenescenceinArabidopsisleaves.PlantJ,2013,75:26–39
[43]BhattramakkiD,DolanM,HanafeyM,WinelandR,VaskeD,RegisterJC3rd,TingeySV,RafalskiA.Insertiondeletionpolymorphismsin3'regionsofmaizegenesoccurfrequentlyandcanbeusedashighlyinformativegeneticmarkers.PlantMolBiol,2002,48:539–547
[44]YangD,YangX,LiuJ,WangBH,LiuBL,WangYZ.PodshatteringresistanceassociatedwithdomesticationismediatedbyaNACgeneinsoybean.NatCommun,2014,5:3352

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