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作物学报 ›› 2017, Vol. 43 ›› Issue (11): 1632-1642.doi: 10.3724/SP.J.1006.2017.01632

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

假禾谷镰孢侵染小麦后3种植物激素相关基因的差异表达分析

  

  • 收稿日期:2017-01-20 修回日期:2017-07-23 出版日期:2017-11-12 网络出版日期:2017-08-01
  • 通讯作者: 丁胜利, E-mail: shengli-ding@163.com; 李洪连, E-mail: honglianli@sina.com
  • 基金资助:

    本研究国家公益性行业(农业)科技专项经费(201503112)资助。

Differential Expression of Three Plant Hormone Related Genes in Wheat Infected by Fusarium pseudograminearum

  1. Henan Agricultural University / National Key Laboratory of Wheat and Maize Crop Science/Collaborative Innovation Center of Henan Grain Crops，Zhengzhou 450002, China?
  • Received:2017-01-20 Revised:2017-07-23 Published:2017-11-12 Published online:2017-08-01
  • Contact: 丁胜利, E-mail: shengli-ding@163.com; 李洪连, E-mail: honglianli@sina.com
  • Supported by:

    This study was supported by China Special Fund for Agro-scientific Research in the Public Interest (201503112).

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摘要：

假禾谷镰孢(Fusarium pseudograminearum)是我国近年新发现的小麦病原真菌，本研究目的是揭示植物激素信号传导途径中相关基因表达对假禾谷镰孢侵染的响应。利用假禾谷镰孢野生菌株WZ2-8A侵染小麦品种周麦24，对接种后5 d和15 d样品进行转录组测序，分析差异表达基因，并对候选基因进行qRT-PCR验证。假禾谷镰孢侵染后，小麦幼苗生长受到明显抑制，根长、株高、根重和地上部分鲜重均显著降低。转录组结果分析表明，植物激素信号传导途径中有29个基因差异表达，涉及到生长素、细胞分裂素和脱落酸3种植物激素。在接种后5 d有11个差异表达基因，其中2个上调，9个下调；在接种后15 d共有25个差异表达基因，其中8个上调，17个下调。在生长素信号传导途径中，生长素输入载体AUX1差异表达，影响生长素的极性运输，从而影响小麦根部的细胞伸长。在细胞分裂素信号传导途径中，起正调控作用的B-ARR上调表达，推测其促进细胞分裂素的信号传导，从而抑制细胞分裂，与生长素协同作用，造成小麦的长势减弱。在脱落酸途径中，脱落酸受体PYR/PYL下调表达；起到负调控作用的PP2C相关基因均上调表达。脱落酸使植物对真菌和细菌的抗性起到负调控作用，其信号传导途径与茉莉酸/乙烯途径相互拮抗，脱落酸信号传导途径的阻遏可能会使茉莉酸/乙烯途径信号通路打开。qRT-PCR结果基本能够和转录组测序结果相拟合，说明在假禾谷镰孢侵染胁迫下，脱落酸的信号传导被抑制可能是中抗品种周麦24对假禾谷镰孢产生一定的抗性的生理基础。

关键词: 小麦, 假禾谷镰孢, 生长素, 细胞分裂素, 脱落酸, 基因差异表达

Abstract:

 

Fusarium pseudograminearum is a pathogenic fungus in wheat newly found in China. The purpose of this study was to disclose the responses of genes involved in the hormone signals pathway to F.pseudograminearum infection. Wheat cultivar Zhoumai 24 wasinfected by the wild-type strain WZ2-8A, and the seedling samples at fiveand fifteendays after inoculation (DAI)were subject to transcriptome analysis by RNA-seqmethod. Theselected differentially expressed genes were subsequently validated by qRT-PCR assay. The infection ofF. pseudograminearum had a negative impact on wheat growth with significantly decreased root length, seedling height, and fresh weights of root and shoot. Atotal of 29 genes expressed differentially in the signal transduction pathways of auxin, cytokinin (CTK) and abscisic acid (ABA). At 5 DAI, 11 genes were expressed differentially compared with the control group (without WZ2-8A inoculation), in whichtwo and nine genes were up- and down-regulated, respectively. At 15 DAI, 25 genes were expressed differentially,including eightup-regulated and seventeendown-regulated genes. In the auxin signaling pathway, auxin input transporter AUX1 was differentially expressed, influencing polar transport of auxin and disturbing cell elongation of wheat roots.In the CTK signaling pathway, the up-regulating role of B-ARR suggests that it couldfacilitate CTK signal transduction to inhibit cellular division,resulting in weaker wheat growth along with the interaction of the auxin signaling pathway. In the ABA signaling pathway, ABA receptor PYR/PYL was down-regulated, while the negative regulator PP2C was up-regulated. ABA plays a negative role in regulating host resistance to fungi and bacteria and its transduction has the antagonistic action against the jasmine acid (JA) / ethylene (ET) pathway, i.e., the repression of ABA signaling pathway might result in the opening ofJA/ET pathway. These results of transcriptome analysis were generally validated bythe qRT-PCR evidence, indicating that inhibition of ABA signal transduction after F. pseudograminearum infection might be the physiological basis of moderate resistance to the fungus in Zhoumai 24.

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