作物学报 ›› 2023, Vol. 49 ›› Issue (7): 1769-1784.doi: 10.3724/SP.J.1006.2023.24180
丁洪艳1,2(), 冯晓溪3, 汪柏宇2, 张积森1,2,*()
DING Hong-Yan1,2(), FENG Xiao-Xi3, WANG Bai-Yu2, ZHANG Ji-Sen1,2,*()
摘要:
LRRII-RLK是一种类受体激酶(receptor-like kinase, RLK), 该基因家族在植物中广泛存在, 在植物的生长发育和生物胁迫中发挥着重要的作用, 但目前在甘蔗中未见对该基因家族的系统鉴定和分析。本研究对甘蔗的LRRII-RLK基因进行分类, 并研究了其结构和功能。结合割手密种基因组和发育组织、叶片发育梯度、昼夜节律和生物胁迫反应转录组数据, 在甘蔗割手密种中共鉴定到27个LRRII-RLK基因, 分布在17条染色体上。系统进化分析发现, 它们分别属NIK、SERK和LRRII-C分支, 其中处于LRRII-C分支的SsLRRII-RLK基因出现了显著扩增。顺式元件分析显示, 它们的启动子共包含32类顺式元件, 其主要参与植物光反应、响应胁迫反应、调控植物激素和植物生长发育。这些基因共有35对共线性基因对, 具有多个保守基序, 主要通过片段复制的方式进行扩增, 且根据Ka/Ks比率发现, 它们在演化过程中经历了严格的纯化选择作用。此外, SsLRRII-RLK基因表达分析显示, 它们在甘蔗不同组织中表达量不同, 其中部分基因在昼夜不同时期表达量呈现差异, 这表明它们参与了植物生长和光合作用的调节。值得注意的是, SsLRRII-RLK基因的表达模式在不同病害(甘蔗梢腐病和花叶病)胁迫下呈现差异, 因此, 我们认为这些基因在病毒复制和发病机制过程中发挥作用。本研究结果有助于深入了解甘蔗LRRII-RLK基因的演化过程, 且为LRRII-RLK基因在甘蔗生长发育、光合作用以及在病害胁迫中的功能研究提供初步的理论基础。
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