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作物学报 ›› 2016, Vol. 42 ›› Issue (04): 525-531 .doi: 10.3724/SP.J.1006.2016.00525

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

紫芽茶树类黄酮生物合成关键酶基因表达与总儿茶素、花青素含量相关性分析

周天山1，王新超2，余有本1，肖瑶1，钱文俊1，肖斌1,*，杨亚军2,*   

  1. 1.西北农林科技大学园艺学院，陕西杨凌712100；2.中国农业科学院茶叶研究所 / 农业部茶树生物学与资源利用重点实验室，浙江杭州310008
  • 收稿日期:2015-09-22 修回日期:2016-01-11 出版日期:2016-04-12 网络出版日期:2016-01-19
  • 通讯作者: 杨亚军, E-mail: yjyang@mail.tricaas.com; 肖斌, E-mail:xiaobin2093@sohu.com
  • 作者简介:杨亚军, E-mail: yjyang@mail.tricaas.com; 肖斌, E-mail:xiaobin2093@sohu.com
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项资助(CARS-23)。

Correlation Analysis between Total Catechins (or Anthocyanins) and Expression Levels of Genes Involved in Flavonoids Biosynthesis in Tea Plant with Purple Leaf

ZHOU Tian-Shan1,WANG Xin-Chao2,YU You-Ben1,XIAO Yao1,QIAN Wen-Jun1,XIAO Bin1*,YANG Ya-Jun2,*   

  1. 1.College of Horticulture, Northwest A&F University, Yangling 712100, China; 2.Key Laboratory of Tea Plant Biology and Resource Utilization, Ministry of Agriculture / Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2015-09-22 Revised:2016-01-11 Published:2016-04-12 Published online:2016-01-19
  • Contact: 杨亚军, E-mail: yjyang@mail.tricaas.com; 肖斌, E-mail:xiaobin2093@sohu.com
  • About author:杨亚军, E-mail: yjyang@mail.tricaas.com; 肖斌, E-mail:xiaobin2093@sohu.com
  • Supported by:

    This study was supported by the China Agriculture Research System (CARS-23).

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被引次数

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摘要：

儿茶素类化合物与花青素均由类黄酮代谢途径合成，紫芽茶中富含花青素。为探明紫芽茶树中类黄酮生物合成代谢流的情况，本试验以来源于湄潭苔茶后代的1株紫色芽叶茶树和1株绿色芽叶茶树为材料，测定芽下第一叶、第二叶和第三叶的叶色、儿茶素类组分和花青素总量，分析了类黄酮生物合成相关的基因表达情况及基因表达量同总儿茶素、花青素累积量之间的相关性。结果表明，紫芽茶树中各叶位中花青素含量均显著高于对照绿芽茶树，而儿茶素类总量却低于对照；类黄酮生物合成关键酶(PAL、CHS、CHI、F3H、DFR、ANS、ANR1、ANR2、F3¢H和F3’5’H)基因均呈现上调趋势。紫色芽叶中的总儿茶素与花青素，同各相关基因(LAR除外)表达水平的相关性都较高，且二者相关系数差异不大。绿色芽叶中的总儿茶素与各基因(LAR、F3’H除外)表达的相关系数，明显高于花青素同各基因表达的相关系数。

关键词: 紫芽茶树, 基因表达分析, 总儿茶素, 花青素, 相关性分析

Abstract:

Flavan-3-ols (aka catechins) and Anthocyanins found in large amounts in tea plant with purple leaf are synthesized through flavonoids metobolic pathway. To investigate the metobolic flux of flavanoids biosynthetic pathway in tea plant with purple leaf, we employed a tea plant with purple leaf and a tea plant with green leaf both from Mei-Tang-Tai-Cha, to examine the expression profiles of related genes involved in flavonoids biosynthesis intensively and determine the concentrations of catechins and anthocynin. Then correlation between total catechin (or anthocyanin) and expression levels of related genes was analyzed. The results indicated that the expression levels of related genes (PAL, CHS, CHI, F3H, DFR, ANS, ANR1, ANR2, F3’H,and F3’5’H) were up-regulated in purple leaves as compared with those in the green leaves. The purple leaf also had higher concentration of anthocyanin than the green leaf, while the green leaf was richer in total catechines. In purple leaves, the expression levels of related genes (except LAR) were highly correlated with both concentrations of total catechines (r = 0.84–0.99) and anthocyanin(r = 0.72–1.00). By contrast, there was only a high correlation between the expression levels of related genes (except LAR and F3¢H) and the concentration of total catechines (r = 0.64–0.77) in green leaves.

Key words: Tea plant with purple leaf, Gene expression analysis, Total catechins, Anthocyanins, Correlation analysis

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