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作物学报 ›› 2012, Vol. 38 ›› Issue (07): 1196-1204.doi: 10.3724/SP.J.1006.2012.01196

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

大豆种质资源叶型和荚粒性状的关系及与SSR标记的关联分析

伍宝朵，陈海峰**，郭丹丹，沙爱华，单志慧，张晓娟，杨中路，邱德珍，陈水莲，朱晓玲，张婵娟，周蓉*，周新安*   

  1. 中国农业科学院油料作物研究所 / 农业部油料作物生物学重点开放实验室, 湖北武汉 430062
  • 收稿日期:2011-10-17 修回日期:2012-02-22 出版日期:2012-07-12 网络出版日期:2012-05-11
  • 通讯作者: 周蓉, E-mail: zhourong@oilcrops.cn, Tel: 027-86735887; 周新安, E-mail: xazhou@public.wh.hb.cn, Tel: 027-86711563
  • 基金资助:

    本研究由国家自然科学基金项目(30871554, 30900906), 中国农业科学院公益性院所科研基金(1610172011006), 作物种质资源保护项目(NB08-2130315-06)和国家转基因生物新品种培育重大专项(2008ZX08004-005, 2009ZX08009-133B, 2009ZX08009-120B)资助。

Relationship of Leaf Shape, Pod Traits and Association with SSR Markers in Soybean Germplasm

WU Bao-Duo**,CHEN Hai-Feng**,GUO Dan-Dan,SHA Ai-Hua,SHAN Zhi-Hui,ZHANG Xiao-Juan,YANG Zhong-Lu,QIU De-Zhen,CHEN Shui-Lian,ZHU Xiao-Ling,ZHANG Chan-Juan,ZHOU Rong*,ZHOU Xin-An*   

  1. Oil Crops Research Institute of Chinese Academy of Agricultural Sciences / Key Laboratory of Oil Crop Biology, Ministry of Agriculture, Wuhan 430062, China
  • Received:2011-10-17 Revised:2012-02-22 Published:2012-07-12 Published online:2012-05-11
  • Contact: 周蓉, E-mail: zhourong@oilcrops.cn, Tel: 027-86735887; 周新安, E-mail: xazhou@public.wh.hb.cn, Tel: 027-86711563

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被引次数

15

摘要： 选用167份国内外大豆品种资源材料，依据叶长/宽比值将其划分为宽叶型和窄叶型, 分析叶型相关性状与荚粒性状的相关性，结果表明叶宽、叶长/宽比均与每荚粒数显著相关(r = –0.69和0.64，P<0.001)。选用均匀分布于大豆20条连锁群上的65个SSR标记分析资源材料的群体结构，并用目标区间内13个SSR标记与叶型相关性状和荚粒性状进行关联分析, 结果显示，标记20-285与每荚粒数显著关联，推测控制每荚粒数的基因可能位于标记20-285附近；标记20-26和20-45间的标记几乎都与叶长/宽比存在显著的关联性，推测控制叶型的基因位于标记20-26和20-45之间。相关分析和关联分析证实大豆叶型与荚粒性状存在密切关系，并缩小了每荚粒数和叶型候选基因的区间。

关键词: 大豆, 叶型, 荚粒性状, 相关性分析, 关联分析

Abstract: Pod traits are important factors affecting soybean yield, and they have been identified to tightly associate with leaf shape. In present study, leaf shape/pod traits were analyzed using 167 soybean accessions, which were classified into two types (broad and narrow leaflets) based on the ratio of leaf length/width. Correlation analysis was carried out between leaf shape related traits and pod traits; ANOVA analysis was also performed using 65 SSR markers, evenly distributed in 20 linkage groups; association analysis was carried out using 13 SSR markers on the target regions. Marker 20-285, locating on chromosome I, highly associated with number of seeds per pod, indicating that the gene controlling number of seeds per pod may co-locate with marker 20-285. Almost all markers between 20-26 and 20-45 were associated with ratio of leaf length/width. We speculated that the genes controlling leaf shape were located in the region from marker 20-26 to 20-45. Hence, it was proved the target region harboring candidate genes of leaf shape and pod number was narrowed. Our study may provide a potential approach in molecular marker-assisted breeding of seeds per pod, and a theoretical guide for cloning the gene of seeds per pod.

Key words: Soybean [Glycine max (L.) Merri], Leaf shape, Pod traits, Correlation analysis, Association analysis

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